Preparation method and application of purely natural product for preventing and treating insomnia
A pure natural, production method technology, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve problems such as limited resources of Cordyceps sinensis, failure to meet market demand, and harsh growth environment requirements for Cordyceps sinensis
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Embodiment 1
[0028] Example 1: Preparation of Cordyceps Adenosine and Cordyceps Polysaccharide Extract
[0029] (a) Pretreatment: take the dried fruiting body of Cordyceps militaris as raw material, and carry out ultra-low temperature pulverization treatment to obtain superfine powder of Cordyceps militaris.
[0030] (b) Water extraction: add a certain amount of extraction solvent to the superfine powder, and perform the extraction operation under a certain temperature condition; combine the extracts and concentrate. The extraction solvent in step (b) is purified water;
[0031] (c) Ethanol extraction: the concentrate obtained in step (b) is dispersed with an appropriate amount of water, an extraction solvent is added, and the extraction operation is performed at an extraction temperature of 0-4°C, and the impregnated extract is centrifuged and collected The supernatant after centrifugation was processed, and the collected supernatant was concentrated and dried by rotary evaporation. The...
Embodiment 2
[0033] Embodiment 2: product making
[0034] This product is made of Cordyceps adenosine and Cordyceps polysaccharide as raw materials, the mass proportion of Cordyceps adenosine is 55%, and the mass proportion of Cordyceps polysaccharide is 45%. According to the requirements of different dosage forms in the later stage, Cordyceps adenosine and Cordyceps polysaccharide can be further processed into tablets, capsules, granules, granules and other series of products in any proportion together with other dosage form excipients.
Embodiment 3
[0035] Embodiment 3: The acute toxicity experiment of this product (pentadenosine) to mice
[0036] Examine the acute toxicity of five types of adenosine samples.
[0037] 【Drugs and Reagents】
[0038] Tested sample: five types of adenosine samples (batch number: 20131121, ingredients: 55% Cordyceps adenosine, 45% Cordyceps polysaccharide, storage method: 4°C refrigeration, preparation method: mix 55% Cordyceps adenosine and 45% Cordyceps polysaccharide after soluble in pure water)
[0039] 【Experimental animals】
[0040]KM mice (18-25g, half male and half female) (animal license number: SCXK (Beijing) 2012-0001, certificate number: 11400700020779), animal sources (Experimental Animal Center, Academy of Military Medical Sciences of the Chinese People's Liberation Army, SCXK (army) 2012-0004), feed source (Experimental Animal Center, Academy of Military Medical Sciences of the Chinese People's Liberation Army, SCXK (Army) 2007-001), feeding environment: normal grade, room te...
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