A kind of method for separation and purification of cannabidiol

A cannabidiol, separation and purification technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve the problems of separation and purification of cannabidiol, and achieves easy recovery and reuse, high separation efficiency, and simple system Effect

Active Publication Date: 2021-09-24
NANJING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Invention idea: The technical idea of ​​the present invention is mainly based on the principle of reverse high performance liquid chromatography, the purpose is to solve the practical problem of separation and purification of cannabidiol in cannabis plant extract or cannabis oil

Method used

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  • A kind of method for separation and purification of cannabidiol
  • A kind of method for separation and purification of cannabidiol
  • A kind of method for separation and purification of cannabidiol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1, take the PS10-100 adsorption material (the particle size is 10-20 μm, the specific surface area is 800-900 m) 2 / g, the aperture is 400-600 A, the hole is 0.7-0.9 cm 3 / g) is a fixed dressing column, the column volume is (column: 7.8 * 300 mm column volume of about 14.32 mL); control the flow rate of 0.3 BV / min, the sample is completed, the column pressure is 1.5 MPa Where the sample sample is a cannabis leaching liquid (chromatogram, figure 1 As shown), the mass fraction of hemp phenol is 0.3%, the impurities are pigments, THC, and oils.

[0042] 2, mixed solution with methanol and water, eluting with methanol / water = 70 / 30, the flow rate is 0.3bV / min, collecting 16 to 23 min; wherein the column pressure is 1.3 during elution. ~ 1.5 mPa, during elution, the chromatogram of the fraction is like image 3 Indicated.

[0043] 3. Evaporation of the fraction in step (2) to obtain a cannipate group, such as a chromatogram Figure 4 As shown, the specific parameters are sh...

Embodiment 2

[0047] 1, take the PS10-100 adsorption material (the particle size is 10-20 μm, the specific surface area is 800-900 m) 2 / g, the aperture is 400-600 A, the hole is 0.7-0.9 cm 3 / g) is a fixed dressing column, the column volume is (column: 7.8 * 300 mm column volume of about 14.32 mL); control the flow rate of 0.3 BV / min, the sample is completed, the column pressure is 1.5 MPa Where the sample is a hemp leaching liquid, wherein the mass fraction of the hemp diphen is 0.3%, the impurities are pigments, THC, and oils.

[0048] 2, mixed solution with methanol and water as a mobile phase, methanol / water = 80 / 20 et al., The flow rate is 0.3bV / min, and the flow rate is 0.3 kin fraction; wherein the column pressure is 1.3 during elution. -1.5 mPa.

[0049] 3. The fraction in step (2) is evaporated to evaporate, which can obtain a hemp phenol product, a purity of 99.2%, and 92% of the extraction rate.

Embodiment 3

[0051] 1. Take the PS10-100 adsorbent material (20-50 μm in the particle size, specific surface area 800-1000 m) 2 / g, the aperture is 400-800 A, and the hole is 0.7-1 cm 3 / g) is a fixed dressing column, the column volume is (column: 7.8 * 300 mm column volume of about 14.32 mL); control the flow rate of 0.3 bv / min, the sample is completed, the column pressure is 1.5 MPa Where the sample is a hemp leaching liquid, wherein the mass fraction of the hemp diphen is 0.3%, the impurities are pigments, THC, and oils.

[0052] 2, using methanol and water mixed solution as mobile phase, methanol / water = 70 / 30 et al., The flow rate is 0.3bV / min, and 16-23 min fraction is collected; wherein the column pressure is 1.2 during elution. -1.4 mPa.

[0053] 3. The fraction in step (2) is evaporated to evaporate, which can obtain hemp phenolic products, 98.9% purity, and 93.2% of the extraction rate.

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Abstract

The invention discloses a method for the separation and purification of cannabidiol. The mother liquor containing cannabidiol is loaded into a chromatographic separation column containing a solid adsorption material, eluted with an eluent, and the eluate is collected. Evaporation and crystallization, that is. The particle size of the solid adsorption material is 5-50μm, and the specific surface area is 400-1000m 2 / g, the pore diameter is 80~1000A, the pore volume is 0.6~1.2cm 3 / g. Advantages: (1) The present invention has low requirements for equipment and is controlled within 3Mpa, which provides the possibility for industrialization; and the separation efficiency is high, and the purity of cannabidiol is higher than 99.5%; (2) One-step purification is adopted, and no need to pass through activated carbon and macropores The adsorption resin removes impurities, which is more convenient. (3) Polymer material is used, which is more resistant to acid and alkali. (4) The amount of eluent is less, when the flow rate of eluent is 0.3BV / min, 6 column volumes can completely elute cannabidiol.

Description

Technical field [0001] The present invention relates to a method of separating purification of cannabis phenol, belonging to the field of separation and purification of bio-based raw materials. Background technique [0002] Cannabis belongs to marijuana marihas a year of herbal plants, widely distributed around the world (cultivated or wild). my country has a long history of marijuana cultivation. At present, the cultivation of marijuana cultivation in my country is mainly concentrated in Yunnan and Northeast, and has rich resources. [0003] Current studies have shown that cannabis rods and cannabis leaves contain more than 70 chemicals known by cannabinoids, of which cannabis phenol (CBD) and tetrahydroacetol (THC) content are the highest. Tetrahydroaja (THC) is an active material component that can enable human inframed, which makes marijuana become the main cause of drugs. However, cannabis phenol (CBD) is a non-toxic and non-addictive active ingredient, or can block the effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C37/82C07C37/68C07C39/23
CPCC07C37/004C07C2601/16C07C39/23
Inventor 江伟魏士明任连兵李俊孙平
Owner NANJING UNIV
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