Method for constructing human pancreatic islet organ model based on organ chip

Abstract

The invention discloses a method for constructing a human pancreatic islet organ model based on an organ chip, particularly a pancreatic islet organ containing multi-cell components, wherein embryoidbodies generated by human induced pluripotent stem cells in situ are induced to be developed into the pancreatic islet organ. The organ chip is a three-dimensional tissue culture chip capable of beingperfused with multiple layers of capsules, wherein differentiation of embryoid bodies into pancreatic islet tissues is promoted by perfusing a differentiation culture medium containing different small molecule inducers while a differentiation induction culture medium is simultaneously poured into upper and lower channels of the chip at a perfusion rate of 100 [mu]L h<-1>. According to the invention, the human-derived pancreatic islet tissue formed by induced differentiation based on the multilayer chip fluid perfusion culture system contains pancreatic island beta cells and pancreatic islandalpha cells, the pancreatic tissue-like cells have good cell viability, can be cultured in vitro for 60 days for a long time and have a good insulin secretion function, pancreas islet tissue development research and pancreas islet pathophysiological mechanism research for in-vitro construction of bionic human pancreas islet tissues are achieved, and a good platform for evaluation and screening ofrelated drugs.

Description

technical field [0001] The invention relates to the technical field of applying microfluidic chip technology to the construction of human-derived organ models in vitro, in particular to a method for constructing human pancreatic islet organoid models based on organ chips. Background technique [0002] In recent years, the incidence of diabetes has risen sharply, and it has become the third largest chronic disease in the world, seriously threatening social health. Diabetes can be divided into type 1 diabetes caused by insulin deficiency and type 2 diabetes caused by insulin resistance according to its pathogenesis. Type 1 diabetes is caused by the autoimmune system destroying the body's pancreatic tissue β cells, resulting in insufficient insulin secretion, which leads to elevated blood sugar in the body. At present, pancreas or islet transplantation is the main method for the fundamental treatment of type 1 diabetes, but the large-scale application of this method in clinica...

AI Technical Summary

Problems solved by technology

Although there are some reports using microfluidic technology to study IPS-induced islets, this method improves the efficiency of islet induction to a certain extent, but it does not solve the fundamental problem of islet transplantation.

Images