Gene editing method for plasmid vector group, dna group and foreign gene introduction

A plasmid vector and foreign gene technology, applied in recombinant DNA technology, other methods of inserting foreign genetic materials, and stably introducing foreign DNA into chromosomes, etc., can solve the introduction position of poultry variation, complex operation, and introduction of foreign genes. Uncertainty and other problems, to achieve the effect of easy construction and easy mass production
CN111269936BActive Publication Date: 2022-07-12ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Publication Date
2022-07-12

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Abstract

The invention belongs to the technical field of genetic engineering and molecular precision improvement and breeding of poultry breeds, and discloses a gene editing method for introducing a plasmid vector group, a DNA group and an exogenous gene, including: P1 plasmid, P2 plasmid and P3 plasmid; DNA components include: The RNA-mediated Cas9 endogenous endonuclease gene, the specific site-mediated sgRNA gene and the site of introduction of the gene fragment and the insertion site-specific homologous DNA fragment. The method of the present invention can integrate the target DNA fragment into the appropriate position of the genome of the poultry organism, and it is helpful to insert the target DNA fragment into the appropriate position of the animal cell genome, thereby obtaining transgenic or chimeric animal individuals. The carrier group of the present invention has the characteristics of easy construction, easy mass production, safety and high efficiency.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering and molecular precision improvement and breeding of poultry varieties, and in particular relates to a gene editing method for introducing plasmid vector groups, DNA groups and exogenous genes. Background technique

[0002] The egg industry is a major characteristic of my country's poultry industry, especially the egg duck industry. Therefore, there are many related breeding studies at home and abroad. However, in the production process of the egg industry, the proportion of male birds used is extremely low (the ratio of male to female in the case of artificial insemination). Only 1:20~30), so the hatching production of male birds during the hatching process is the biggest waste of resources in the development of the egg and poultry industry. Therefore, the question is how to use the latest gene editing technology to introduce foreign genes for the labeling of fertilized eggs of male bird...

Claims

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