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The application of adenosine in the prevention and treatment of honey bee virus infection

A bee and adenosine technology, applied in the field of bee breeding, can solve the problems of low production efficiency, high production cost, and restriction of mass production of antimicrobial peptides, and achieve the effects of improving survival rate, reducing copy number, and contributing to normal growth and development.

Active Publication Date: 2021-03-30
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chinese patent CN103524602A discloses that exogenous antimicrobial peptides are used to treat bee cystic larvae, but the administration of exogenous antimicrobial peptides still faces the potential safety risks of antimicrobial peptides, the mass production of antimicrobial peptides is limited, and the production efficiency is low. Problems such as low and high production costs (Lin Chengde, Peng Hongjuan, Wang Yanhai. The application and existing problems of antimicrobial peptides. Journal of Tropical Medicine. 2007,1(7):86-90.)

Method used

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  • The application of adenosine in the prevention and treatment of honey bee virus infection
  • The application of adenosine in the prevention and treatment of honey bee virus infection
  • The application of adenosine in the prevention and treatment of honey bee virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Utilizing adenosine to intervene healthy Chinese bee larvae and poisoned larvae

[0044] 1. Samples for testing

[0045] Apis cerana comes from the experimental bee colony of the Honey Bee Research Institute of the Chinese Academy of Agricultural Sciences. In order to obtain 2-day-old larvae, the queen bee was kept on the spleen to lay eggs for 96 hours, and the 2-day-old larvae were taken out from the colony and placed in a 24-well plate, placed in a temperature of 32±1°C and relative humidity. Perform pre-cultivation in a constant temperature and humidity incubator at 75±5% RH.

[0046] Reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the existence of CSBV and other viruses in the test larvae (including black queen bee virus BQCV, defective virus DWV, Israeli paralysis virus IAPV, Kashmir virus KBV, acute paralysis virus ABPV, For chronic paralysis virus (CBPV) infection, healthy larvae without virus infection are used as ...

experiment example 1

[0067] Experimental example 1 Analysis of the survival rate and mortality of healthy larvae and infected larvae after adenosine intervention

[0068] 1. Survival rate

[0069] Survival rate statistics are carried out to each group of honeybee larvae in embodiment 1, and the results are as follows: figure 1 As shown, the results showed that the survival rate of larvae in the CK group and the preparation group fed conventionally could reach more than 86%, indicating that adenosine intervention would not cause abnormal death of healthy larvae and would not affect the growth and development of healthy larvae.

[0070] A large number of larvae in the infection group died, and the survival rate was only 43%. The survival rate of the larvae in the intervention group was significantly increased to more than 76%. Among them, the low concentration group (0.75 μg / mL) intervened in the 6-day-old larvae and all survived. The results showed that using Adenosine intervention can save about ...

experiment example 2

[0078] Experimental example 2 Detection of virus copy number after adenosine intervention in healthy larvae and infected larvae

[0079] CSBV detection is carried out to the apparently healthy 4-6 day-old Chinese bee larvae (CK group) in Example 1, and it is found that the virus copy number of each day-old larva is 66-70, indicating that the apparently healthy larvae also have virus infection, but did not show obvious symptoms, indicating that CSBV is generally invisible to infection in bee colonies, and the larvae can survive with the virus within a certain concentration, which is consistent with Liu Shan (Shan, L., Liuhao, W., Jun, G., Yujie ,T.,Yanping,C.,Jie,W.,Jilian,L.,Chinese Sacbrood Virus Infection in Asian Honey Bees(Apis cerana cerana)and HostImmune Responses to the Virus Infection,Journal of Invertebrate Pathology.2017,doi:http: / / dx.doi.org / 10.1016 / j.jip.2017.09.006) are consistent with the research results. After adenosine intervention, the virus copy number in ...

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Abstract

The invention relates to the technical field of bee breeding, and in particular relates to an application of adenosine in prevention and treatment of bee virus infection. The inventors find that the adenosine can significantly improve the survival rate of apis cerana larvae infected with Chinese sacbrood virus (CSBV) and reduce the mortality rate. The adenosine can inhibit the proliferation of theCSBV in bees and reduce the copy number of the virus; and at the same time, the adenosine can also induce the expression of endogenous antibacterial peptides and improve the innate immune defense ofthe bees. The adenosine can be used to prepare drugs or feed for preventing and treating CSBV infection, has important significance for the prevention and treatment of Chinese bee sacbrood and has good market application value.

Description

technical field [0001] The invention relates to the technical field of bee breeding, in particular to the application of adenosine in the prevention and treatment of bee virus infection. Background technique [0002] Bees are important pollinators in nature. About one-third of the crops in the world need bees to pollinate to some extent, while some fruits and vegetables are completely dependent on bees for pollination. Bee pollination can significantly improve the yield and quality of crops, so it has important economic value in agricultural production. In addition, bees can also produce a wide variety of peak products, such as honey, royal jelly, propolis, bee pollen, etc. The quality of bee products is closely related to the health of bees. There are potential safety hazards in bee products produced by sick bees, and drugs for treating bee diseases will also remain in bee products, which will lead to safety problems in bee products. [0003] Chinese honey bee (Apis ceran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7076A61P37/04A61P31/14A23K50/90A23K20/153
CPCA61K31/7076A23K20/153A23K50/90A61P31/14A61P37/04
Inventor 孙丽萍李继莲张雪琦侯春生徐书法杨术鹏李熠
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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