A kind of composite bacterial agent for strengthening the anaerobic degradation of butyric acid and its construction method

Abstract

The invention discloses a composite bacterial agent for strengthening the anaerobic degradation of butyric acid and a construction method thereof. The composite bacterial agent includes fatty acid oxidizing bacteria Syntrophomonas wolfei and electrogenic bacteria Geobactorsulfurreducens. The present invention activates the fatty acid oxidizing bacteria Syntrophomonas wolfei and the electrogenic bacteria Geobactorsulfureducens, uses the anaerobic bottle to insert the electrode as the experimental mode, and adds the activated bacterial liquid into the anode chamber to construct a mutual symbiosis system that strengthens the degradation of butyric acid, The composite bacterial agent can be used in the process of sewage treatment; this method has the advantages of simple structure, flexible operation, quick start, stable operation, no need for aeration and low operating cost, and can be controlled by pH value, ammonia nitrogen and initial butyric acid concentration. Optimal conditions for regulation of butyric acid degradation.

Description

Technical field [0001] The present invention belongs to the field of anaerobic fermentation technology, and more relates to a microbial cell, and more particularly to a compound fungicide with anaerobutic degradation of hematipate and its construction method thereof. Background technique [0002] Anaerobic biotechnology, due to its characteristics of the reduction and capacity of organic matter in wastewater, it can be used to deal with high concentration organic wastewater and achieve better practical results. The basic principle is to achieve step-by-step degradation and methane of organic matter by hydrolyzing fermentation bacteria, acetate and methane bacillus. One of the main challenges of conventional anaerobic processes is highly stable and stable, which is the problem of acid inhibition caused by volatile fatty acids, VFAS. During the operation of the traditional anaerobic device, the aforementioned theoretical speed of the soil-hydrolysis of the organic acid produces an ...

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Problems solved by technology

The above research results show that the abundance of mutualistic symbionts of short chain fatty acid-oxidation syntrophs (SFAS) and methanogens in the anaerobic system can be enhanced by adding SFAS and acetic acid-type methanogens. However, there are mainly the following challenges: the target functional bacteria through external dosing can not achieve the enrichment target in the process of competition with the indigenous flora, especially in the case of continuous feeding and discharging. The effect of flora; the method of placing functional microorganisms in the anaerobic system through continuous dosing or cell immobilization technology has achieved certain results, but this method increases the operational difficulty and operating cost of actual operation and has a great impact on engineering technology and The quality requirements of management personnel are high, and the feasibility of actual operation is poor; in addition, from the perspective of microbial isolation and metabolic pathways, there are currently few acid-resistant methanogens that can be isolated and cultured, and acid-resistant methanogens can be obtained even after low-pH domestication. Methanogens can strengthen the recovery of the acidification system, but they cannot fundamentally change the inherent physiological characteristics of methanogens, such as the growth generation time and slow metabolic rate, and the best carbon source for methanogens under acidic conditions Possibly transformed into methanol and thus weakened the ability to degrade VFAs

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