Test kit for detecting African swine fever virus and method for detecting African swine fever virus
A technology of African swine fever virus and kit, applied in the field of biological detection, can solve the problems of reducing the accuracy of detection results, false positives, false negatives, etc., achieve broad market prospects and commercial value, easy operation, and short time-consuming effects
Inactive Publication Date: 2020-06-19
ZHEJIANG UNIV
8 Cites 3 Cited by
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Abstract
The invention provides a test kit for detecting African swine fever virus. The kit comprises a plasmid containing an ASFV structural protein gene VP73 fragment; an upstream primer of the VP73, a downstream primer of the VP73, an upstream primer of the beta-Actin and a downstream primer of the beta-Actin; a probe of VP73, a probe of beta-Actin. The test kit is easy and convenient to operate, low inprice and short in consumed time, has high sensitivity and specificity and has wide market prospects and commercial value. The invention also provides a method for detecting the African swine fever virus, which comprises the following steps: taking a plasmid containing an ASFV structural protein gene VP73 fragment as a positive control, and taking a porcine actin gene beta-Actin as an internal reference control gene; monitoring from the initial stage of detection, so that the whole virus detection process can be monitored, the effect of internal reference quality control is perfectly embodied, and the accuracy of the detection result is improved.
Application Domain
Microbiological testing/measurementDNA/RNA fragmentation
Technology Topic
Classical swine feverVirus detection +7
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