In-vitro fermentation system and evaluation system applying same

Abstract

The invention discloses an in-vitro fermentation system. The in-vitro fermentation system comprises a frame, and an incubator and a control cabinet which are arranged on the frame; the control cabinetis provided with a control unit; fermentation tanks are arranged in the incubator; the temperatures in the fermentation tanks are controlled through water bath heating in culture grooves; the controlunit acts on a magnetic stirring device to stir the fermentation tanks; the control unit controls work of peristaltic pumps through microcomputer intelligent PID to automatically feed acid or alkali;the incubator is connected with a vacuum pump, an oxygen tank and a nitrogen tank; and an electromagnetic valve, a carbon dioxide sensor and an oxygen sensor are electrically connected with the control unit so as to regulate the carbon dioxide content on line and realize culture in a micro-oxygen and anaerobic environment. The whole detailed evaluation process from sample collection to selectiveculture of genes, metabolites and viable bacteria is established, and the results of metagenome are analyzed by a customized software system, so that the whole evaluation process is greatly shortened.The invention better solves the problems in the prior fermentation process and can adapt to various requirements for fermentation culture.

Description

technical field [0001] The invention relates to the technical field of flora culture, in particular to an in vitro fermentation system and an evaluation system using the system. Background technique [0002] Intestinal flora plays an important role in human health. Intestinal flora imbalance is closely related to a variety of diseases. Current studies have found that various factors such as diet, drugs, natural products, and small molecules all affect the structure and metabolism of intestinal flora. have an impact, and play a role in the health of the body through these changes. [0003] One of the methods used in existing studies is based on the analysis of groups of intervention factors. The shortcoming is that the human body is a complex organism, which can neither completely rule out the interference of other influencing factors nor distinguish whether the intervention factors directly regulate the flora. Or indirectly regulate the flora by affecting the host. [0004...

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Problems solved by technology

[0003] One of the methods used in existing studies is based on the analysis of groups of intervention factors. The shortcoming is that the human body is a complex organism, which can neither completely rule out the interference of other influencing factors nor distinguish whether the intervention factors directly regulate the flora. Or indirectly regulate the flora by affecting the host

[0006] The existing in vitro fermentation equipment and methods have the following deficiencies: 1. Most of the existing in vitro fermentation equipment are only used for fermentation and are bulky, which cannot realize multiple simultaneous evaluations; 2. Most of them are liquid supplements, regardless of drugs, food, small The evaluation of molecular or bacterial powder on bacterial flora intervention requires a solid feeding port; 3. Lack of anaerobic working environment and special equipment that integrates fermentation and anaerobic work; 4. There is no perfect system from sampling, fermentation evaluation to flora gene A complete evaluation system for community, metabolites and live bacteria culture; 5. The evaluation method of flora takes too long

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