Recombinant Escherichia coli producing melatonin and its construction method and application

Abstract

The invention discloses a recombinant escherichia coli producing melatonin, a construction method and application thereof. The present invention firstly discloses recombinant Escherichia coli, compared with the recipient Escherichia coli, the expression level of the gene related to melatonin biosynthesis is increased and / or the content of the melatonin biosynthesis-related protein is increased and / or the The activity of the melatonin biosynthesis-related protein is increased; the melatonin is selected from at least one of the following: phenylalanine hydroxylase, dihydromonophosphate reductase, 4a-hydroxytetrahydrobiopterin dehydratase, Aspartate transaminase family proteins, N‑acetyltransferase and caffeic acid‑O‑methyltransferase. The construction method and application of the recombinant Escherichia coli are further disclosed. The melatonin-producing recombinant Escherichia coli strain of the invention has significantly higher melatonin synthesis efficiency than other existing strains, and is of great significance to the industrialized production and large-scale application of melatonin.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a melatonin-producing recombinant Escherichia coli and its construction method and application. Background technique [0002] Melatonin (N-acetyl-5-methoxytryptamine, melatonin, C 13 N 2 h 16 o 2 ) belongs to tryptophan derivatives and is an important antioxidant in organisms. Melatonin was first discovered in the pineal gland of cattle and is considered to be an important neurohormone. Later research found that melatonin is distributed in various organs in the human body and plays different important functions. Now it has been widely used in medicine In the field of health care, it is used to treat symptoms such as insomnia and depression. In 2019, the global market demand for melatonin reached 1 billion US dollars. Melatonin synthesis pathways widely exist in natural organisms, and the classic synthesis pathway in cells is: tryptophan → 5-hydroxytryptophan → 5-...

AI Technical Summary

Problems solved by technology

Although the biosynthetic pathway of melatonin is very clear, the proteins involved in it have low specific activities, and most of them are difficult to express in the existing mature expression system, basically forming inclusion bodies or the expression level is extremely low, so so far Are there any engineered bacteria that produce high melatonin?

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