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Application of platycodon polysaccharide in antagonizing apoptosis induced by fumonisin b1 through autophagy

A technology of platycodon polysaccharides and alveolar macrophages, applied to medical preparations containing active ingredients, antifungal agents, drug combinations, etc., can solve the problems of unpredictable effects of cell damage, unpredictability, cell apoptosis, etc.

Active Publication Date: 2021-11-02
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, the molecular weight, chemical composition, glycosidic bond, conformation, branching degree, etc. of polysaccharides will affect the activity of polysaccharides. Therefore, the activity of polysaccharides is different and unpredictable; on the other hand, different types Different inducers have different mechanisms of cell damage, therefore, even the same substance has unpredictable effects on cell damage caused by different inducers
So far, there is no report about platycodon polysaccharides antagonizing fumonisin B1-induced apoptosis through autophagy

Method used

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  • Application of platycodon polysaccharide in antagonizing apoptosis induced by fumonisin b1 through autophagy
  • Application of platycodon polysaccharide in antagonizing apoptosis induced by fumonisin b1 through autophagy
  • Application of platycodon polysaccharide in antagonizing apoptosis induced by fumonisin b1 through autophagy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the preparation of bellflower polysaccharide PGSt

[0053] Cut platycodon root into slices, add 10 times the weight of water to soak overnight, then extract at 90°C for 3 hours, filter, concentrate the filtrate to a relative density of 1.05-1.15 (measured at 50°C), and centrifuge to remove impurities to obtain the extract liquid;

[0054] Add ethanol with a volume concentration of 95% to the extract, stir while adding, so that the final concentration of ethanol is 70%, let stand, centrifuge, separate the precipitate, and dry the precipitate at 60°C to prepare PGPS 70 Add ethanol with a volume concentration of 95% to the supernatant after separation and precipitation, and stir while adding, so that the final concentration of ethanol is 50%, let stand, centrifuge, separate and precipitate, and dry the precipitate at 60°C to prepare PGPS 50 ; the PGPS 70 and PGPS 50 Mix evenly to prepare platycodon polysaccharide PGSt.

Embodiment 2

[0055] Example 2: Platycodon grandiflorum antagonizes fumonisin B1-induced apoptosis of 3D4 / 21 cells through autophagy

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Abstract

The invention discloses the use of platycodon polysaccharide in antagonizing cell apoptosis induced by fumonisin B1 through autophagy. The study of the present invention finds that the toxic effect of FB1 on alveolar macrophages is mainly reflected in the induction of cell apoptosis. Platycodon polysaccharide induces autophagy through the two signaling pathways of PI3K / Akt / mTOR and MEK / ERK, and then antagonizes the apoptosis of alveolar macrophages induced by FB1 through autophagy, thereby achieving the protective effect on alveolar macrophages. The invention clarifies the regulatory mechanism of platycodon polysaccharides in the crosstalk between alveolar macrophage apoptosis and autophagy, and provides a theoretical basis for the development of therapeutic drugs for corresponding diseases.

Description

technical field [0001] The invention belongs to the field of medical applications of platycodon polysaccharides, in particular to the use of platycodon polysaccharides in antagonizing cell apoptosis induced by fumonisin B1 through autophagy. Background technique [0002] Fumonisin B1 (FB1) is a mycotoxin produced by the fungus Fusarium verticillioides, commonly associated with maize. It produces species-specific and organ-specific toxicities, including leukoencephalomalacia in horses, pulmonary edema in pigs, and liver or kidney damage in most animal species. [0003] Alveolar macrophages are an important part of the lung's resistance to pathogenic infection. When in contact with pathogenic factors, alveolar macrophages stretch out pseudopodia and surround them until they form closed bodies, and then the bodies detach from the cell membrane to form cytoplasm The phagosome finally fuses with the lysosome in the cell, which contains a variety of proteases that can digest path...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P11/00A61P31/10A61K36/346
CPCA61K31/715A61K36/346A61P11/00A61P31/10
Inventor 赵晓娜刘建柱程国栋邓干臻李克鑫李克钦刘国涛李雯
Owner SHANDONG AGRICULTURAL UNIVERSITY