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Serum protein marker for indicating healthy aging key pathway and application thereof

A protein and marker technology, applied in the field of biomedicine, can solve problems such as inability to industrialize, no technical means to evaluate healthy aging, and complex technical means.

Inactive Publication Date: 2020-07-03
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the current technical means, the technical means to evaluate the changes of each pathway are very complicated, involving a large number of precise experimental means, and it is impossible to carry out industrialization and process operation
However, there is currently no technical means to assess one or more key pathways involved in healthy aging

Method used

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  • Serum protein marker for indicating healthy aging key pathway and application thereof
  • Serum protein marker for indicating healthy aging key pathway and application thereof
  • Serum protein marker for indicating healthy aging key pathway and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Key pathways of healthy aging based on serum protein markers

[0015] Through mass spectrometry quantitative data analysis of serum proteome of 119 long-lived elderly (95-111 years old, median 100 years old) and 151 elderly control populations (32-80 years old, median 61 years old) in Hainan Province, The present invention provides a key pathway of healthy aging based on serum protein and a serum protein marker indicating the key pathway of healthy aging, wherein the key pathway of healthy aging based on serum protein includes 12 up-regulated pathways and 4 down-regulated pathways:

[0016] a), Up-regulated pathways are:

[0017]Wound healing; Hemostasis, blood coagulation, fibrin clot formation; Humoral immune response; Defense response to bacteria; Acute inflammatory response (acute inflammatory response); Binding and Uptake of Ligands by Scavenger Receptors; axon development; bone growth; ossification; vascular development (blood vessel development); axon...

Embodiment 2

[0024] Example 2 Serum Protein Markers Indicating Key Pathways of Healthy Aging in Example 1

[0025] The present invention also provides a new class of serum protein markers that indicate key pathways of healthy aging. These markers can be used alone or in combination of two or more to assess changes in key pathways of healthy aging, including 193, Among them, the 158 serum protein markers up-regulated include:

[0026]α-2 macroglobulin (A2, M Alpha-2-macroglobulin), plasma protease C1 inhibitor (SERPING1, Plasma protease C1 inhibitor), collagen α-1 (I) chain (COL1A1, Collagen alpha-1 (I) chain), collagen α-2 (I) chain (COL1A2, Collagen alpha-2 (I) chain), collagen α-1 (V) chain (COL5A1, Collagen alpha-1 (V) chain), cartilage oligomerization Matrix protein (COMP, Cartilage oligomericmatrix protein), dystrophin (DAG1, Dystroglycan), type A ephrin receptor 4 (EPHA4, Ephrintype-A receptor 4), coagulation factor VIII (F8, Coagulation factor VIII), granulin Precursor (GRN, Granu...

Embodiment 3

[0046] Example 3 Carry out assessment of healthy aging with bone growth (bone growth) this pathway:

[0047] The specific implementation method is as follows:

[0048] 1. Professional medical staff draw blood from at least 3 subjects (at least 3 samples are required to calculate the average protein abundance), obtain serum samples by centrifugation, and store them at -80°C;

[0049] 2. Measure the expression abundance of apolipoprotein L1 in serum samples: For example, through the relative quantitative method based on the mass spectrometry platform (Q-Exactive) - TMT, to obtain the 4 proteins contained in the functional enrichment of bone growth: COL6A1 , COL6A3, COL12A1, COMP expression abundance, the detailed steps are as follows:

[0050] a). Protein extraction: Serum samples were taken out from -80°C, centrifuged at 12,000g for 10 minutes at 4°C to remove cell debris, and the supernatant was transferred to a new centrifuge tube. The kit produced by Thermo was referred to ...

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Abstract

The invention discloses a serum protein marker for indicating healthy aging key pathways and application. The number of the serum protein markers is 193, and the key pathways comprise 12 up-regulationpathways and 4 down-regulation pathways. The invention provides a novel serum protein marker for indicating a blood health aging key pathway for the first time. By measuring changes of the serum protein markers, changes of channels related to healthy aging in a human body can be comprehensively evaluated, the risk of non-healthy aging of elderly individuals is further evaluated, and theoretical support is provided for subsequent intervention and response.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a serum protein marker indicating a key pathway of healthy aging and its application. Background technique [0002] The aging rate of my country's population is more than twice that of western countries, and the number of people over 65 years old will increase from 119 million in 2010 to 360 million in 2050 (accounting for 25.6% of the total population). The intensification of the aging process and the high incidence of senile diseases have seriously affected the quality of life of Chinese people and increased the burden on families and society. However, if the health of the elderly improves by 1% relative to the previous year, the total cost of family care in my country in 2050 will save 2.21 trillion yuan. The potential savings from healthy aging are even more staggering when you add in medical costs. Considering that geriatric diseases are often difficult to ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2800/60
Inventor 孔庆鹏尹藩乾李功华
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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