Intestinal tract simulation chip and application thereof

Abstract

The invention discloses an intestinal tract simulation chip and an application thereof. The intestinal tract simulation chip comprises a culturing layer, an elastic membrane layer and a pneumatic layer, wherein the culturing layer comprises a culturing chamber, and is used for culturing cells to be cultured, and a fine hair structure is arranged on the inner wall on one side of the culturing chamber; the elastic membrane layer comprises an elastic membrane having air permeability; the pneumatic layer comprises a gas chamber and a pneumatic control pipeline which communicates with the gas chamber; the elastic membrane is covered on the gas chamber; and the pneumatic layer is at least used for driving a local region of the elastic membrane to produce deformation and / or displacement which tends to the culturing chamber, and then the inner wall on which the fine hair structure is arranged, of the culturing chamber, produces corresponding deformation and / or displacement. According to the intestinal tract simulation chip disclosed by the invention, simulation of the fine hair structure of the intestinal tract, simulation of intestinal peristalsis and simulation of intestinal tract dissolved oxygen environment can be realized on the same device.

Description

technical field [0001] The invention relates to an intestinal simulation chip, in particular to an intestinal simulation chip capable of simulating intestinal villi, intestinal peristalsis and intestinal dissolved oxygen environment and its application, which belongs to the application field of microfluidic technology in organ chips. Background technique [0002] In recent years, the research on organ chips has made great progress, and has important application prospects in the fields of new drug research and development, stem cell research, tissue and organ development, and toxicology prediction. It was listed as one of the "Top Ten Emerging Technologies" by the Davos Forum in 2016 one. Researchers have realized the construction of many human organs on microfluidic chips, such as liver-on-a-chip, lung-on-a-chip, intestine-on-a-chip, kidney-on-a-chip, blood vessel on a chip, heart on a chip, and multiple organ-on-a-chip. The Dutch biotechnology company Mimetas has developed...

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Problems solved by technology

[0004] From 2012 to 2015, Donald E.Ingber et.al designed a biomimetic "human gut chip" microdevice consisting of two microfluidic channels separated by a porous flexible membrane coated with extracellular matrix (ECM) Opened and lined by human intestinal epithelial (Caco-2) cells, simulating the complex structure of living intestines, and generating low shear stress on the microchannels with low-velocity fluid flow, by applying cyclic strains that simulate physiological peristaltic motion (10%, 0.15Hz) to rebuild the intestinal microenvironment. Under these conditions, the columnar epithelium develops rapid polarization, spontaneously grows into folds, reproduces the structure of intestinal villi, and forms a high-integrity barrier small molecule. The chip is an integrated chip , the culture layer and the pneumatic layer are integrated and cannot be disassembled. The assembly process is relatively complicated, the sample volume is small, and the convincing biological phenomena of the reaction are not convincing enough. Although the chip can simulate physiological peristalsis, it cannot be produced under this condition. The villi are very different from actual intestinal villi not only in height but also in radius

Although the chip simulates the fluid mechanics of the intestinal tract and the structure of the villi, it cannot simulate the physiological peristalsis of the small intestine, and the assembly is relatively troublesome, the manufacturing process is relatively more complicated, and the cultivation must be in this bioreactor, which is relatively difficult to operate

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