Enzymoimmune reagent kit and its production method

A kit and enzyme immunology technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as interference and high detection costs, and achieve the effects of simplified operation, high sensitivity, and stable colored products

Inactive Publication Date: 2003-07-09
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the diagnostic kits for detecting TORCH-IgM by indirect enzyme immunosorbent assay in the world include: the products of Maxim Biotechnology Company of the United States, HOPE Company, and Microbix Company of Canada. The above kits can only be used in different plate wells. The IgM of the four pathogens of TOX, RV, CMV and HSV are detected respectively in the above method. The above methods have three common shortcomings. One is the interference of IgM type rheumatoid factor (IgM-RF), which is essentially IgM against self IgG, antigen Form a complex with its specific IgG and combine with IgM-RF to cause a false positive reaction; second, specific IgG can competitively occupy the binding site between IgM and antigen to produce a false negative reaction; It costs about 160 yuan

Method used

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Embodiment Construction

[0050] specific implementation plan

[0051] The invention comprises a box body, a porous strip arranged in the box body and a liquid used in the box body, and each hole of the porous strip is coated with the TORCH antigen that can cause fetal congenital malformation to detect TORCH in blood of pregnant women -IgM antibody, prepare and mix the coating solution and other solutions for later use, first use the coating solution (0.05mol / L, pH9.6 carbonic acid buffer plus 0.003mol / L urea and 0.003mol / L guanidine hydrochloride) Dilute the TORCH antigen so that the final concentrations are: TOX antigen 10 μg / 100ml, RV antigen 170 μg / 100ml, CMV antigen 110 μg / 100ml, HSV-1 antigen 110 μg / 100ml. Place the TORCH antigen suspension at 37°C for 2 hours, then coat the strips, 100 μl / well, place in a refrigerator at 4°C for 6-12 hours, then shake off the coating solution, wash once with washing solution, and then seal with blocking solution , 110 μl / well, and put in a refrigerator at 4°C f...

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Abstract

An enzymonimmune reagent kit for testing the IgM antibodies of 4 prenatal pathogens (TOX, RV, CMV and HSV) for gravida is composed of box, perforated plate, oprating liquid including washing liquid, enclosing liquid, diluting liquid, enzyme substrate and enzyme-linked matter resisting human IgM, and the liquid sealed in the holes on plate, which contains dicarbonate, sodium carbonate, urea, guanidine hydrochloride and distilled water for test antigen of TORCH rivus and TORCH-IgM antibody. Its advantages are easy operation and high effect.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to the technical field of IgM antibody detection of pathogens in pregnant women. Background technique [0002] In order to do a good job in family planning and implement prenatal and postnatal care, it is generally necessary to detect TORCH (toxoplasma, rubella virus, cytomegalovirus, herpes simplex virus) specific IgM antibodies after women become pregnant. At present, the diagnostic kits for detecting TORCH-IgM by indirect enzyme immunosorbent assay in the world include: the products of Maxim Biotechnology Company of the United States, HOPE Company, and Microbix Company of Canada. The above kits can only be used in different plate wells. The IgM of the four pathogens of TOX, RV, CMV and HSV are detected respectively in the above method. The above methods have three common shortcomings. One is the interference of IgM type rheumatoid factor (IgM-RF), which is essentially I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/535
Inventor 严华
Owner YANGZHOU UNIV
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