Duraglutin artificial antigen and preparation method thereof
A technology of artificial antigen and doloretine, which is applied in chemical instruments and methods, animal/human peptides, animal/human proteins, etc., can solve the problems of high cost, restricting the actual development and market demand of rapid detection of meperidines, etc. To achieve the effect of simple preparation process, good specificity and strong antibody specificity
A technology of artificial antigen and doloretine, which is applied in chemical instruments and methods, animal/human peptides, animal/human proteins, etc., can solve the problems of high cost, restricting the actual development and market demand of rapid detection of meperidines, etc. To achieve the effect of simple preparation process, good specificity and strong antibody specificity
CN111423358AInactive Publication Date: 2020-07-17HANGZHOU BIOTEST BIOTECH CO LTD
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[0031] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings. It should be noted that the specific implementation is only a detailed description of the present invention and should not be regarded as a limitation of the present invention.
[0032] (1) Preparation of pemeridine artificial hapten:
[0033] (a) Weigh 500mg (ie 2mmol) 4-phenylpiperidine-4-carbonitrile, add it to a 150ml single-necked round bottom flask, then add 25ml acetone, 1g potassium carbonate, 431mg (ie 3mmol) methyl iodide, add and stir After the reaction, the solvent was evaporated to dryness under reduced pressure, filtered, and the filtrate was extracted with 3×10ml ethanol, filtered and spin-dried again to obtain 678 mg of white solid product (2); purified by thin-layer chromatography, TLC : Chromatography solution is ethyl acetate, product R f =0.5~0.7;
[0034] (b) In a 50ml single-necked round bottom flask, add 300mg ...
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Abstract
The invention provides a duramine artificial antigen and a preparation method thereof. The nitrile group of the 4-phenylpiperidine-4-carbonitrile is ingeniously reduced into the amino group; carboxylgroups capable of being used for crosslinking are introduced, so the characteristic original appearance of a duraglutin main ring is maintained; butanedioic anhydride is introduced, so acetyl functional groups on a duraglutin side chain is maintained on the said chain; the hapten synthesized by the process can maintain the characteristic group original appearance of duraglutin, and the synthesizedcorresponding artificial antigen has good specificity. The preparation process is simple, and the raw material source is not limited; the prepared artificial antigen can be used for animal immunization to obtain a corresponding duraglutin antibody, has strong antibody specificity, can be used in immunoassay, is suitable for research of various duraglutin immunoassay methods, and can be used as akey raw material for production of a duraglutin immunochromatographic kit.
Description
technical field [0001] The invention belongs to the technical field of biochemical engineering and immune detection, and in particular relates to a peperidine artificial antigen and a preparation method thereof. Background technique [0002] Pethidine, also known as meperidine, demerol; meperidine; meperidine, is a synthetic opioid receptor agonist, belonging to phenylpiperidine derivatives, and is a synthetic analgesic for clinical application. Molecular formula: C 15 h 21 NO 2 , molecular weight: 247, structural formula: [0003] [0004] Demerol is an artificial substitute of morphine, its action and mechanism are similar to morphine, and it has similar properties to morphine. The pharmacological effect is the same as that of morphine, and the clinical application is also the same as that of morphine. Repeated effects of pethidine can be addictive, and its adverse reactions are similar to morphine, and it is listed as a strictly controlled narcotic drug. [0005]...
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Patent Timeline
17 Jul 2020
Publication
CN111423358A
- IPC
- C07D211/26; C07K14/765; C07K14/795; C07K14/77; C07K14/47; C07K16/44
- CPC
- C07D211/26; C07K14/47; C07K14/765; C07K14/77; C07K14/795; C07K16/44; C07K19/00
- Inventors
- 吴淑江; 王百龙