Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Duraglutin artificial antigen and preparation method thereof

A technology of artificial antigen and doloretine, which is applied in chemical instruments and methods, animal/human peptides, animal/human proteins, etc., can solve the problems of high cost, restricting the actual development and market demand of rapid detection of meperidines, etc. To achieve the effect of simple preparation process, good specificity and strong antibody specificity

Inactive Publication Date: 2020-07-17
HANGZHOU BIOTEST BIOTECH CO LTD
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, few biotechnology companies in China have successfully developed immunoassay detection reagents for peperidine drugs of abuse and key raw materials for the production of such kits (demeridine antigens, antibodies), because pemeridine artificial antigens still rely on high-cost and high-risk The synthesis process of the original drug of pethidine, the raw material of pethidine is controlled by various countries, which restricts the actual development and market demand of pethidine rapid detection, so it is very important to develop and develop a new and effective synthetic method of pethidine artificial antigen necessary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Duraglutin artificial antigen and preparation method thereof
  • Duraglutin artificial antigen and preparation method thereof
  • Duraglutin artificial antigen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings. It should be noted that the specific implementation is only a detailed description of the present invention and should not be regarded as a limitation of the present invention.

[0032] (1) Preparation of pemeridine artificial hapten:

[0033] (a) Weigh 500mg (ie 2mmol) 4-phenylpiperidine-4-carbonitrile, add it to a 150ml single-necked round bottom flask, then add 25ml acetone, 1g potassium carbonate, 431mg (ie 3mmol) methyl iodide, add and stir After the reaction, the solvent was evaporated to dryness under reduced pressure, filtered, and the filtrate was extracted with 3×10ml ethanol, filtered and spin-dried again to obtain 678 mg of white solid product (2); purified by thin-layer chromatography, TLC : Chromatography solution is ethyl acetate, product R f =0.5~0.7;

[0034] (b) In a 50ml single-necked round bottom flask, add 300mg ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention provides a duramine artificial antigen and a preparation method thereof. The nitrile group of the 4-phenylpiperidine-4-carbonitrile is ingeniously reduced into the amino group; carboxylgroups capable of being used for crosslinking are introduced, so the characteristic original appearance of a duraglutin main ring is maintained; butanedioic anhydride is introduced, so acetyl functional groups on a duraglutin side chain is maintained on the said chain; the hapten synthesized by the process can maintain the characteristic group original appearance of duraglutin, and the synthesizedcorresponding artificial antigen has good specificity. The preparation process is simple, and the raw material source is not limited; the prepared artificial antigen can be used for animal immunization to obtain a corresponding duraglutin antibody, has strong antibody specificity, can be used in immunoassay, is suitable for research of various duraglutin immunoassay methods, and can be used as akey raw material for production of a duraglutin immunochromatographic kit.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering and immune detection, and in particular relates to a peperidine artificial antigen and a preparation method thereof. Background technique [0002] Pethidine, also known as meperidine, demerol; meperidine; meperidine, is a synthetic opioid receptor agonist, belonging to phenylpiperidine derivatives, and is a synthetic analgesic for clinical application. Molecular formula: C 15 h 21 NO 2 , molecular weight: 247, structural formula: [0003] [0004] Demerol is an artificial substitute of morphine, its action and mechanism are similar to morphine, and it has similar properties to morphine. The pharmacological effect is the same as that of morphine, and the clinical application is also the same as that of morphine. Repeated effects of pethidine can be addictive, and its adverse reactions are similar to morphine, and it is listed as a strictly controlled narcotic drug. [0005]...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D211/26C07K14/765C07K14/795C07K14/77C07K14/47C07K16/44
CPCC07D211/26C07K14/47C07K14/765C07K14/77C07K14/795C07K16/44C07K19/00
Inventor 吴淑江王百龙
Owner HANGZHOU BIOTEST BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com