Duraglutin artificial antigen and preparation method thereof

Abstract

The invention provides a duramine artificial antigen and a preparation method thereof. The nitrile group of the 4-phenylpiperidine-4-carbonitrile is ingeniously reduced into the amino group; carboxylgroups capable of being used for crosslinking are introduced, so the characteristic original appearance of a duraglutin main ring is maintained; butanedioic anhydride is introduced, so acetyl functional groups on a duraglutin side chain is maintained on the said chain; the hapten synthesized by the process can maintain the characteristic group original appearance of duraglutin, and the synthesizedcorresponding artificial antigen has good specificity. The preparation process is simple, and the raw material source is not limited; the prepared artificial antigen can be used for animal immunization to obtain a corresponding duraglutin antibody, has strong antibody specificity, can be used in immunoassay, is suitable for research of various duraglutin immunoassay methods, and can be used as akey raw material for production of a duraglutin immunochromatographic kit.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering and immune detection, and in particular relates to a peperidine artificial antigen and a preparation method thereof. Background technique [0002] Pethidine, also known as meperidine, demerol; meperidine; meperidine, is a synthetic opioid receptor agonist, belonging to phenylpiperidine derivatives, and is a synthetic analgesic for clinical application. Molecular formula: C 15 h 21 NO 2 , molecular weight: 247, structural formula: [0003] [0004] Demerol is an artificial substitute of morphine, its action and mechanism are similar to morphine, and it has similar properties to morphine. The pharmacological effect is the same as that of morphine, and the clinical application is also the same as that of morphine. Repeated effects of pethidine can be addictive, and its adverse reactions are similar to morphine, and it is listed as a strictly controlled narcotic drug. [0005]...

AI Technical Summary

Problems solved by technology

At present, few biotechnology companies in China have successfully developed immunoassay detection reagents for peperidine drugs of abuse and key raw materials for the production of such kits (demeridine antigens, antibodies), because pemeridine artificial antigens still rely on high-cost and high-risk The synthesis process of the original drug of pethidine, the raw material of pethidine is controlled by various countries, which restricts the actual development and market demand of pethidine rapid detection, so it is very important to develop and develop a new and effective synthetic method of pethidine artificial antigen necessary

Images