Preparation method and application of a zirconium-based metal-organic framework colorimetric array sensor

An array sensor and organic framework technology, applied in the field of analytical chemistry, can solve the problem that the detection system cannot perform multiple analyzes at the same time, and achieve the effects of low production cost, stable reaction system, and easy operation

Active Publication Date: 2022-01-11
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many assay systems cannot be multiplexed simultaneously due to the limitations of specific binding interactions between proteins and receptors

Method used

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  • Preparation method and application of a zirconium-based metal-organic framework colorimetric array sensor
  • Preparation method and application of a zirconium-based metal-organic framework colorimetric array sensor
  • Preparation method and application of a zirconium-based metal-organic framework colorimetric array sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Detection of various proteins using a colorimetric array sensor that interacts with zirconium-based metal-organic frameworks and single-stranded DNA-modified gold nanoparticles

[0039] Step 1: Preparation of zirconium-based metal-organic framework: ZrCl 4 (120 mg), aminoterephthalic acid (110 mg) and benzoic acid (1.9 g) were dissolved in 10 mL of DMF and transferred to a Teflon liner. After ultrasonic treatment for 3 minutes, put it in 120°C and let it stand for 24 hours to react. The resulting product was obtained by centrifugation and washed repeatedly three times with fresh DMF and methanol (8000 rpm, 5 minutes). Finally, to remove the residual organic solvent, the nanoparticles were collected after drying overnight at 65 °C.

[0040]Step 1: Preparation of gold nanoparticles: When the chloroauric acid solution (50 mL, 1 mM) was heated to boiling, trisodium citrate solution (5 mL, 38.8 mM) was quickly added under vigorous stirring. Continue to heat the...

Embodiment 2

[0043] Example 2: Detection of a variety of semen samples using a colorimetric array sensor interacting with zirconium-based metal-organic frameworks and single-stranded DNA-modified gold nanoparticles

[0044] Step 1: Preparation of zirconium-based metal-organic framework: ZrCl 4 (120 mg), aminoterephthalic acid (110 mg) and benzoic acid (1.9 g) were dissolved in 10 mL of DMF and transferred to a Teflon liner. After ultrasonic treatment for 3 minutes, put it in 120°C and let it stand for 24 hours to react. The resulting product was obtained by centrifugation and washed repeatedly three times with fresh DMF and methanol (8000 rpm, 5 minutes). Finally, to remove the residual organic solvent, the nanoparticles were collected after drying overnight at 65 °C.

[0045] Step 1: Preparation of gold nanoparticles: When the chloroauric acid solution (50 mL, 1 mM) was heated to boiling, trisodium citrate solution (5 mL, 38.8 mM) was quickly added under vigorous stirring. Continue to ...

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Abstract

The invention relates to a preparation method and application of a colorimetric array sensor of a zirconium-based metal-organic framework and single-stranded DNA modified gold nanoparticles. Six different single-stranded DNA sequences were designed to modify the surface of gold nanoparticles to form unique sensor recognition elements. The new material zirconium-based metal-organic framework can bind and precipitate single-stranded DNA sequence modification on gold nanoparticles through different intermolecular forces. After adding certain proteins or semen samples, their binding force can be accelerated or slowed down, affecting It aggregates to produce a detectable signal. Therefore, we can consider that these recognition elements have different interactions with different targets, and we can detect different targets obviously by analyzing the colorimetric response of each recognition element in combination. The invention can be used for the colorimetric array analysis of various proteins and male semen simply and efficiently, and provides detection basis for the analysis of male infertility.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a preparation method and application of a colorimetric array sensor of zirconium-based metal-organic frameworks and single-stranded DNA-modified gold nanoparticles [0002] Background of the invention [0003] Male infertility is a social disease that plagues male compatriots. Rapid and accurate identification of sperm motility is the key to diagnosis and formulation of targeted treatment strategies. In addition to known factors, there are still 30-45% of patients whose semen parameters are abnormal and the cause has not been found out. These abnormal cases are collectively referred to as idiopathic male infertility. Thresholds for normal ranges have not been clearly defined due to the lack of available data on the most direct samples of semen variables. Moreover, because of the complexity and diversity of samples, it brings many limitations to the detection. Detection of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31C08G83/00
CPCG01N21/31C08G83/008
Inventor 李根喜孙召伟吴帅马洁桦潘艳红韩祎巍
Owner NANJING UNIV
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