Composition of three kinds of lactobacillus and application of composition

A composition, the technology of Lactobacillus gasseri, applied in the field of medicine, can solve the problems of high number of viable bacteria, difficult effect of a single strain, poor colonization ability, etc., and achieve obvious colonization effect, active and stable biological characteristics, and colonization ability strong effect

Pending Publication Date: 2020-07-31
广东龙创基药业有限公司 +2
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The strains of live bacterial preparations currently on the market are not the dominant vaginal flora of women in my country. They have poor colonization ability, poor stability, and cannot maintain a high number of viable bacteria, so they cannot fully meet the clinical needs.
There are many kinds of Lactobacillus in the vagina of healthy women. At present, there is only a single strain of the drug in the drug, but it is difficult for a single strain to play a good role in regulating the vaginal flora. Even if a single strain has good acid production ability, Produce H 2 o 2 ability, and the ability to adhere to vaginal epithelial cells, but a single strain also has difficulty in recolonizing into the vaginal flora, and it is difficult to exert long-term effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition of three kinds of lactobacillus and application of composition
  • Composition of three kinds of lactobacillus and application of composition
  • Composition of three kinds of lactobacillus and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the preparation of Lactobacillus rhamnosus RD-0060 freeze-dried bacterial powder

[0051] Lactobacillus rhamnosus RD-060 was grown in broth liquid medium (MRS) at pH 6.5, fermented in a 100-liter scale fermenter, and cultured anaerobically. The bacteria were collected in the early plateau stage, and the number of viable bacteria reached about 1.0×10 9 More than CFU / ml. Collect the thalli by centrifugation, wash with phosphate buffer, and mix with lyoprotectant (including skimmed milk powder and sucrose, etc.). Then, the mixture was freeze-dried in a freeze dryer. Samples were frozen at -40°C for approximately 2 hours, then vacuum dried at -20°C for 20-30 hours, and then dried at 30°C for 3-5 hours. After the freeze-dried powder is packaged, and refrigerated storage. The lyophilized powder is counted for live bacteria, and the number of live bacteria should be 1.0×10 10 CFU / g or more.

Embodiment 2

[0052] Embodiment 2, the preparation of Lactobacillus gasseri RD-0046 freeze-dried bacterial powder

[0053] Lactobacillus gasseri RD-0046 was grown in broth (MRS) at pH 6.5, fermented in a 100 liter scale fermenter, and anaerobically cultured. The bacteria were collected in the early plateau stage, and the number of viable bacteria reached about 1.0×10 9 More than CFU / ml. Collect the thalli by centrifugation, wash with phosphate buffer, and mix with lyoprotectant (including skimmed milk powder and sucrose, etc.). Then, the mixture was freeze-dried in a freeze dryer. Samples were frozen at -40°C for approximately 2 hours, then vacuum dried at -20°C for 20-30 hours, and then dried at 30°C for 3-5 hours. After the freeze-dried powder is packaged, and refrigerated storage. The lyophilized powder is counted for live bacteria, and the number of live bacteria should be 1.0×10 10 CFU / g or more.

Embodiment 3

[0054] Embodiment 3, the preparation of Lactobacillus jansnii RD-0135 freeze-dried bacterial powder

[0055] Lactobacillus jansnii RD-0135 was grown in broth liquid medium (MRS) at pH 6.5, fermented using a 100 liter scale fermenter, and anaerobically cultured. The bacteria were collected in the early plateau stage, and the number of viable bacteria reached about 1.0×10 9 More than CFU / ml. Collect the thalli by centrifugation, wash with phosphate buffer, and mix with lyoprotectant (including skimmed milk powder and sucrose, etc.). Then, the mixture was freeze-dried in a freeze dryer. Samples were frozen at -40°C for approximately 2 hours, then vacuum dried at -20°C for 20-30 hours, and then dried at 30°C for 3-5 hours. After the freeze-dried powder is packaged, and refrigerated storage. The lyophilized powder is counted for live bacteria, and the number of live bacteria should be 1.0×10 10 CFU / g or more.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Viable countaaaaaaaaaa
Viable countaaaaaaaaaa
Live bacteriaaaaaaaaaaa
Login to view more

Abstract

The invention discloses a composition composed of three kinds of lactobacillus. The composition contains a lactobacillus rhamnosus strain, a lactobacillus gasseri strain and a lactobacillus jensenii strain, and can resist bacterial vaginosis and related vaginal infection. The composition has a remarkable effect on the bacterial vaginosis and related vaginal diseases, is safe and non-toxic, has good stability, and can be stored for a long time. Compared with other commercially available preparations at present, the strains in the composition can be effectively planted, and the three kinds of lactobacillus strains can play a synergistic role; and compared with a single strain preparation, the composition composed of the three kinds of lactobacillus has the advantage that vaginal microecologycan be formed more obviously.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a composition composed of three kinds of lactobacillus and its application, especially for preparing medicine for treating gynecological diseases. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecological system that restricts, coordinates and dynamically balances with the host and the environment. The vaginal flora of healthy women is mainly composed of Lactobacillus, including Lactobacillus rhamnosus, Lactobacillus jansneri, Lactobacillus gasseri, Lactobacillus crispatus, and Lactobacillus vaginalis. Under normal circumstances, Lactobacillus can protect the vagina. When the Lactobacillus in the vaginal microecology is disturbed, it may lead to the invasion of pathogenic bacteria and various diseases caused by the imbalance of vaginal flora. [0003] The occurrence of bacterial vagin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A61K35/747A61P15/00A61P15/02A61P31/04A61P31/10A61P31/12C12R1/225
CPCC12N1/20A61K35/747A61P15/00A61P15/02A61P31/04A61P31/10A61P31/12A61K2300/00Y02A50/30
Inventor 龚莉蒋彬陈桂歆张佳冬许少燕许雪芸李煜龙
Owner 广东龙创基药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap