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Embryo-tissue-culture rapid propagation method for epimedium sagittatum

A technology of tissue culture rapid propagation and epimedium, applied in the field of plant tissue culture, to achieve the effect of perfecting the disinfection process, increasing the rate, and increasing the induction rate

Active Publication Date: 2020-08-04
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few reports on the tissue culture technology of Epimedium sagittarius, and there are few reports on the use of epimedium sagittarius embryos to induce dedifferentiation of callus and then differentiate into sterile seedlings for plant tissue culture. Huohu breeding technology laid a solid foundation

Method used

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  • Embryo-tissue-culture rapid propagation method for epimedium sagittatum

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Embodiment 1

[0050] Embodiment 1: a kind of Epimedium sagittatum embryo tissue culture rapid propagation method, its steps are as follows:

[0051] (1) Disinfection of dried seeds: Select healthy and plump seeds from the seed collection bag after removing grass leaves and other impurities, tear off the seed film on the seeds, wash with detergent solution at a ratio of 1:500, and place Shake in a shaking box at 120rpm for 1 hour to remove the dust attached to the surface, rinse with clean water 3 or 4 times to remove residual detergent; move it into an ultra-clean workbench, then shake and disinfect with 75% alcohol solution for 30 seconds, rinse with sterile water 2 times, put the seeds in 0.1% HgCl 2 In the solution, shake and disinfect at 120rpm for 10min, take it out and rinse it with sterile water for 3 or 4 times to remove residual HgCl 2 solution; then place it on sterilized filter paper to remove residual water for later use.

[0052] (2) Seed low-temperature cultivation: inoculat...

Embodiment 2

[0058] Embodiment 2: a kind of Epimedium sagittatum embryo tissue culture rapid propagation method, its steps are as follows:

[0059] (1) Disinfection of dried seeds: Select healthy and plump seeds from the seed collection bag after removing grass leaves and other impurities, tear off the seed film on the seeds, wash with detergent solution at a ratio of 1:500, and place Shake in a shaking box at 120rpm for 1 hour to remove the dust attached to the surface, rinse with clean water 3 or 4 times to remove residual detergent; move it into an ultra-clean workbench, then shake and disinfect with 75% alcohol solution for 30 seconds, rinse with sterile water 2 times, put the seeds in 0.1% HgCl 2 In the solution, shake and disinfect at 120rpm for 10min, take it out and rinse it with sterile water for 3 or 4 times to remove residual HgCl 2 solution; then place it on sterilized filter paper to remove residual water for later use.

[0060] (2) Seed low-temperature cultivation: inoculat...

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Abstract

The invention relates to the technical field of plant tissue culture and particularly discloses an embryo-tissue-culture rapid propagation method for epimedium sagittatum. The method comprises the steps of taking an embryo of the epimedium sagittatum as an explant, carrying out procedures such as complete-dried seed disinfection, seed low-temperature culture, explant callus generation inducing, callus adventitious-bud or cluster-bud differentiation inducing, bud fibril formation inducing, seedling hardening and seedling raising so as to obtain regenerated seedlings of epimedium sagittatum, andestablishing a rapid propagation system for plant tissue culture of the epimedium sagittatum. The method has the advantages that the operation is simple, the pollution rate is low, the cycle of propagation is short, the rate of propagation is high, the cost is low, and the field survival rate of young seedlings is high, so that a technical support is provided for factory production of the epimedium sagittatum, and the method has a certain promoting action on protecting wildlife resources of the epimedium sagittatum.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically relates to a method for tissue culture and rapid propagation of epimedium sagittarius embryos. Background technique [0002] Epimedium, a traditional Chinese medicine, is the dried leaf of Epimedium in the Berberidaceae family (Berberidaceae), and has been used as a medicinal material in my country for more than 2,000 years. At present, the acquisition of Epimedium medicinal materials mainly relies on wild picking. Extinct mining has drastically reduced the wild resources of Epimedium medicinal materials, and the contradiction between supply and demand has become increasingly serious. Epimedium sagittatum (Sieb.et Zucc.) Maxim. is one of the basic plants of Epimedium sagittatum in the "Chinese Pharmacopoeia", and it is widely used. At present, the artificial planting of this species has been carried out in many provinces stand up. Seedling breeding technology ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁琼张燕君于淑霞黄天悦于东悦王兰香
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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