Method for calculating metabolic levels of different brain regions after spinal cord injury by <13>C labeled glucose

Abstract

The invention discloses a method for calculating metabolic levels of different brain regions after spinal cord injury by <13>C labeled glucose. The method comprises the following steps: (a), manufacturing a spinal cord injury model by adopting different methods and instruments according to experimental requirements; (b), catheterizing the caudal vein of the rat, injecting [1-<13>C] glucose, killing the animal by a microwave radiation method, and separating a target brain region; (c), preparing a target brain area NMR sample, and collecting NMR original data; (d), performing normalization processing on the original data; (e), performing metabolite concentration calculation; and (f), calculating metabolic abundance. Therefore, the technical problems that isotope markers are accumulated andcannot continue to participate in the metabolic process, related information of metabolic pathways and metabolic intervals can not be provided and the sensitivity is low in the prior art are solved, and the method for calculating the metabolic concentration and metabolic abundance of the cerebral cortex substances after spinal cord injury is simple and high in result obtaining accuracy.

Description

technical field [0001] The invention belongs to the technical application field of stable isotope labeling glucose, in particular to a 13 C-labeled glucose method for calculating metabolic levels in different brain regions after spinal cord injury. Background technique [0002] The traditional method to study brain metabolism after spinal cord injury is a biochemical method using 14C or 18F labeled 2-deoxyglucose (2-DG) as a tracer. In recent years, 13 C NMR methods can give more detailed information about metabolic pathways and metabolic intervals in the study of brain metabolism. Labeled 2-deoxyglucose (glucose analogue) can be transported from capillaries to cells like glucose, and phosphorylated in the cytoplasm to generate the intermediate product 2-deoxyglucose-6-phosphate (2-DG-P) . Labeled 2-DG-P cannot be further metabolized, accumulates in cells, and can be detected by corresponding autoradiography (detection of 14C labeling) and positron electron tomography (P...

AI Technical Summary

Problems solved by technology

However, MRS cannot provide relevant information on metabolic pathways and metabolic intervals, and is not suitable for samples with low concentrations, and its sensitivity is not high

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