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Application of wild jujube leaf total flavonoid extract in preparation of medicine for preventing and treating alteration of intestinal flora

A technology of intestinal flora imbalance and sour jujube leaves, which is applied in the field of medicine and can solve the problems of single regulation

Active Publication Date: 2020-08-07
汉方萃取生物科技(海南)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the drugs used to regulate the intestinal flora are probiotic drugs such as Bifidobacterium triple live bacteria powder, compound Lactobacillus acidophilus tablets, Bacillus subtilis dual live bacteria enteric-coated capsules, and lactobacillus tablets. The regulatory function of the flora is single, all of which directly supplement normal physiological bacteria, regulate the intestinal flora, and inhibit the fungi and pathogenic bacteria that are potentially harmful to the human body in the intestinal tract

Method used

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  • Application of wild jujube leaf total flavonoid extract in preparation of medicine for preventing and treating alteration of intestinal flora
  • Application of wild jujube leaf total flavonoid extract in preparation of medicine for preventing and treating alteration of intestinal flora
  • Application of wild jujube leaf total flavonoid extract in preparation of medicine for preventing and treating alteration of intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Preparation of total flavonoid extract of wild jujube leaves

[0042] Weigh 1000g wild jujube leaves, add 50% (volume concentration) ethanol 12L, soak for 3h, reflux for 2 times, 0.5h each time, filter, combine the filtrate, and evaporate the solvent to obtain the wild jujube leaf extract solid, top D101 macroporous resin, Loading concentration 0.5g·mL -1 , Pass through the resin column at a flow rate of 0.5 BV / h, wash away impurities with distilled water, and then eluted with 50% (volume concentration) ethanol at a flow rate of 1 BV / h. Collect the eluent and evaporate the solvent to obtain 92g of Jujube leaf flavonoid extract powder . The purity of the total flavonoids of Jujube leaves in the obtained total flavonoids of Jujube leaves was 40.95% determined by ultraviolet spectrophotometry.

Embodiment 2

[0043] Example 2: Determination of the total flavonoids extract of wild jujube leaves

[0044] Experimental equipment and materials:

[0045] Experimental instrument: Waters 2695A lliance system, including quaternary pump, vacuum online degasser, autosampler, Waters 2489 ultraviolet detector, solid phase extraction cartridge (Shengshi Sage Technology Co., Ltd.).

[0046] Reagents: methanol (Tianjin Damao Chemical Reagent) is analytically pure, methanol and acetonitrile (TEDIA USA) are chromatographic alcohols.

[0047] Drugs: Rutin (batch number: 100080-200707) purchased from China Institute for Food and Drug Control, eriocitrin (batch number: 20161216), hyperoside (batch number: 20161022), quercetin 3-O-locoside ( Batch number: 20161227), isoquercetin (batch number: 20160830), kaempferol 3-O-rutin (batch number: 20170417) were purchased from Baoji Chenguang Biotechnology Company.

[0048] Weigh about 10 mg of the total flavonoid extract powder of wild jujube leaves prepared in Example...

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Abstract

The invention provides application of a wild jujube leaf total flavonoid extract in preparation of a medicine for preventing and treating alteration of intestinal flora. According to the invention, rats with alteration of intestinal flora caused by D-galactose are taken as research objects, and the intestinal flora regulation effect of the wild jujube leaf total flavone extract is studied. Rat experiment results show that supplementing of the wild jujube leaf total flavonoid extract can prevent alteration of intestinal flora in the D-galactose induced aging process, for example, increase of intestinal flora diversity, rise of bacteroides, decrease of phylum palustre, and decrease of lactobacillus are realized, and the proportion and composition of phylum palustre and bacteroides tend to anormal control group.

Description

Technical field [0001] The invention belongs to the field of medicine, and specifically relates to the application of the total flavonoids extract of jujube leaves in preparing medicines for preventing and treating intestinal flora imbalance. Background technique [0002] Intestinal microbes are an important "microbial organ" of the human body and are closely related to many physiological functions such as digestion, immunity, nutrition, metabolism, and cognition. The types and quantities of intestinal microbes undergo subtle changes, which in turn affect the physiological functions of the body. [0003] At present, the drugs used to regulate the intestinal flora are mostly probiotic drugs such as Bifidobacterium triple live bacteria powder, compound Lactobacillus acidophilus tablets, Bacillus subtilis double live bacteria enteric-coated capsules, and lactic acid bacteria tablets. The regulating effect of the flora is single, which directly supplements normal physiological bacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P1/00A23L33/105A61K127/00
CPCA61K36/725A61P1/00A23L33/105A61K2236/333A61K2236/39A61K2236/55A23V2002/00A23V2250/2116A23V2200/3204
Inventor 杜晨晖闫艳付彩崔小芳裴香萍
Owner 汉方萃取生物科技(海南)股份有限公司
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