Super-resolution microscopic imaging method and microscope

Abstract

The embodiment of the invention discloses a super-resolution microscopic imaging method and a microscope. The super-resolution microscopic imaging method comprises the following steps: carrying out expansion treatment on a biological sample; slicing the expanded biological sample to form a biological sample slice; forming a structured light illumination light sheet by using the diffraction-free light beam, and obtaining a super-resolution single image of the structured light illumination light sheet; and fusing all the super-resolution single images to obtain a super-resolution image of the biological sample. According to the technical scheme provided by the embodiment of the invention, the problems that the existing microscopic imaging speed is too low and the existing microscopic imagingspeed is not suitable for carrying out fluorescence in-situ sequencing on a large-volume and high-flux biological sample can be solved, and rapid and high-precision microscopic imaging of the biological sample is realized.

Description

technical field [0001] Embodiments of the present invention relate to microscopic imaging technology, and in particular to a super-resolution microscopic imaging method and a microscope. Background technique [0002] Limited by the diffraction effect of light, the resolution of optical microscopy is limited to about half of the detection wavelength, about 200nm. In order to explore more life activities that occur between subcellular and molecular complexes, super-resolution microscopy imaging technology with higher resolution has been developed rapidly in recent years. [0003] In the prior art, super-resolution microscopy based on single-molecule localization is a commonly used microscopy technique, including Stochastic Optical Reconstruction Microscopy (STORM for short) and Photoactivated Localization Microscopy (Photoactivated Localization Microscopy). Microscopy, referred to as PALM), its imaging resolution can reach 20nm×20nm×50nm. However, its imaging speed is slow, ...

AI Technical Summary

Problems solved by technology

However, its imaging speed is slow, and the super-resolution image of a single frame may require hundreds or even thousands of original images to be synthesized.

Moreover, due to the need for repeated activation and quenching processes, the phototoxicity is high, and it is not suitable for fluorescent in situ sequencing (merfish) of large-volume, high-throughput biological samples.

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