Bionic biomineralized artificial bone repair material as well as preparation method and application thereof

A technology of biomineralization and repair materials, applied in tissue regeneration, drug delivery, pharmaceutical formulations, etc., can solve the problems of excessive degradation speed, poor hydrophilicity, and poor binding degree, achieve a good three-dimensional space structure, improve mechanical Performance, good biocompatibility effect

Active Publication Date: 2018-04-24
BEIJING PAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The research on bone scaffolds at home and abroad mainly focuses on the composite scaffolds of hydroxyapatite and collagen. Although CN1562387A, CN101229392A, CN101417145A, etc. have produced various types of collagen / hydroxyapatite composite bone scaffolds, due to the existence of such materials Loose structure, relatively poor strength, very weak toughness, too fast degradation rate, etc., can not match the bone growth rate well, making it difficult to become a good scaffold for bone tissue engineering
Existing studies have shown that the spatial structure of three-dimensional scaffolds is the basis of bone repair materials and bone conduction properties. performance of composite materials, but there are still problems such as high brittleness, scattered orientation of hydroxyapatite microcrystals, and poor bonding with collagen. Moreover, these scaffolds are formed by compaction, and no porous structure is formed. Morphology, which is not conducive to cell adhesion and proliferation
CN102205150A, CN103785059A, and CN102240415A use polylactic acid or its copolymers as the matrix to prepare bone materials with a three-dimensional void structure. Although they have good mechanical properties, the hydrophilic properties of the materials are poor, and the degradation products will continue to produce acidic substances, causing poor biocompatibility
CN105457097A, CN101554493A use the anti-collapse agent, dispersant and / or modifier etc. to fully mix to obtain nano-hydroxyapatite / collagen scaffold, but the anti-collapse agent, dispersant and / or modifier etc. in the scaffold are non-natural Components of bone structure, affecting material structure and osteogenesis
[0006] In summary, the field needs a three-dimensional bone repair scaffold material with good bionic effects, but it has not been effectively satisfied for a long time. Therefore, it is urgent to develop a new bone repair scaffold material to meet the actual needs

Method used

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  • Bionic biomineralized artificial bone repair material as well as preparation method and application thereof
  • Bionic biomineralized artificial bone repair material as well as preparation method and application thereof
  • Bionic biomineralized artificial bone repair material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] In the preparation of the biomimetic biomineralized artificial bone in this example, hydrochloric acid was used as the acid solution, and CaCl was used as the calcium ion solution. 2 Aqueous solution, alkaline solution using NaOH aqueous solution, using ultraviolet radiation physical cross-linking method. figure 1 Shown is the flow chart of the preparation method of the biomimetic biomineralized artificial bone of the present invention.

[0078] according to figure 1 The steps shown, the preparation method of biomimetic biomineralization artificial bone is:

[0079] Step S1 (preparation of biomineralized collagen / hydroxyapatite composite powder) specifically includes:

[0080] Step S1-1 (dissolving collagen): dissolving 2 g of collagen in 1000 mL of hydrochloric acid solution with a concentration of 0.001 mol / L to prepare an acid solution of collagen, wherein the concentration of collagen is 0.2%, and the pH of the solution is 3;

[0081] Step S1-2 (adding calcium ...

Embodiment 2

[0097] In the preparation of the biomimetic biomineralized artificial bone in this example, hydrochloric acid was used as the acid solution, and CaCl was used as the calcium ion solution. 2 Aqueous solution, alkaline solution using NaOH aqueous solution, using glutaraldehyde for chemical crosslinking.

[0098] The mineralized collagen composite porous material was prepared according to steps S1-1 to S3-2 in Example 1, and then the mineralized collagen composite porous material was chemically crosslinked using glutaraldehyde, specifically, the mineralized collagen composite porous material obtained in step S3-2 was The mineralized collagen composite porous material was soaked in 0.05wt% glutaraldehyde in anhydrous ethanol solution for 48 hours for cross-linking; then the cross-linked mineralized collagen composite porous material was taken out, placed in a chromatography column, and purified by flowing pure Washing with water for 48 hours to remove the residual cross-linking ag...

Embodiment 3

[0100] In the preparation of the biomimetic biomineralized artificial bone in this embodiment, the acid solution adopts nitric acid aqueous solution, and the calcium ion solution adopts Ca(NO 3 ) 2 Aqueous solution, alkaline solution using KOH aqueous solution, using thermal dehydrogenation method for physical cross-linking.

[0101] The biomimetic biomineralized artificial bone was prepared according to the same steps as in Example 1, except that the acid solution in step S1-1 was replaced by 1000 mL of nitric acid aqueous solution with a concentration of 0.001 mol / L for hydrochloric acid solution, and the calcium ion solution in step S1-2 was replaced by 20 mL Concentration of 0.1mol / L Ca(NO 3 ) 2 Aqueous solution instead of CaCl 2 Aqueous solution, the alkaline aqueous solution in step S1-4 and step S2-3 replaces NaOH aqueous solution with the KOH aqueous solution of concentration 0.01mol / L, after step S3-2, use thermal dehydrogenation method to mineralized collagen comp...

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Abstract

The invention provides a bionic biomineralized artificial bone repair material as well as a preparation method and an application thereof. The material consists of a cross-linked collagen matrix and biomineralized collagen/hydroxyapatite composite powder dispersed in the matrix, wherein the mass ratio of the collagen matrix to the biomineralized collagen/hydroxyapatite composite powder is (9-1) to(1-9). The bionic biomineralized artificial bone repair material provided by the invention is a collage-based bionic biomineralized artificial bone, which can meet a demand of artificial bone and tissue suturing in a clinical GTR or GBR operation; the artificial bone repair material consists of hydroxyapatite and pure collagen, and the two ingredients are major ingredients of human natural tissues, so that the artificial bone repair material is free from toxicity; and influence to a material structure and an osteogenesis effect is avoided, and good biocompatibility is guaranteed. Due to the existence of the cross-linked collagen matrix, problems of a material which can get dispersed easily, and is too high in degradation speed, poor in mechanical performance and the like can be solved; and the bionic construction of nano-hydroxyapatite and collagen is achieved.

Description

technical field [0001] The invention relates to a bionic biomineralized artificial bone and a preparation method and application thereof, belonging to the field of biomedical materials. Background technique [0002] Clinically, bone defects are mainly caused by trauma, infection, and tumors. In response to this situation, people have prepared a variety of substitute materials for bone repair through various methods or approaches, and achieved certain results. [0003] Bone implant materials can be roughly divided into autologous bone, allograft bone, xenograft bone, and synthetic materials. Autologous bone transplantation is rarely used due to the limitation of its own donors; although the bone formation effect of allograft bone is better, it also has the risk of rejection and virus infection, and because of limited sources, it is difficult to achieve a large number of patients in clinical practice. Use; Xenograft bone is rarely used due to the large rejection reaction. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/24A61L27/12
CPCA61L27/12A61L27/24A61L27/56A61L2400/12A61L2430/02C08L89/06
Inventor 张自强张以河陈飞旭
Owner BEIJING PAISHENG BIOTECH CO LTD
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