Restriction-site associated DNA sequencing library construction method, restriction-site associated DNA sequencing data analysis method, detection equipment and storage medium

Abstract

The invention discloses a construction method of a restriction-site associated DNA sequencing library, an analysis method of restriction-site associated DNA sequencing data, detection equipment and astorage medium. The construction method comprises the following steps: carrying out enzyme digestion treatment on genome DNA to form a plurality of DNA fragments; respectively connecting the two endsof each DNA fragment with linkers to form a DNA fragment sample; selecting a DNA fragment sample with preset design length from the DNA fragment samples; carrying out a second round of enzyme digestion treatment on the selected DNA fragment sample, and extracting effective cell nucleus genome sequence DNA fragments; carrying out PCR amplification on the effective cell nucleus genome sequence DNA fragments by using a primer; and selecting a target genome sequence DNA fragment from the effective genome sequence DNA fragments having undergone PCR amplification to obtain an original DNA fragment of restriction-site associated DNA sequencing. Through embodiments of the invention, a genome simplification degree is realized, the number of detectable polymorphic sites is pre-judged, the density and uniformity of the distribution of a detected target region in the genome is evaluated and analyzed, and the construction of the restriction-site associated DNA sequencing library is flexibly and accurately carried out.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a method for constructing a simplified genome sequencing library, a method for analyzing simplified genome sequencing data, detection equipment and a storage medium. Background technique [0002] At present, the detection of genetic characteristics at the genome-wide level among individuals of a certain species is one of the current international research hotspots in animal and plant genomics. This is of great significance to the study of the evolutionary history of species, environmental adaptability, natural selection, genetic map construction, linkage analysis of target traits, and precise positioning of trait QTLs. In order to improve the detection intensity, detection accuracy and detection accuracy of the above research, it is usually necessary to find high-density single nucleotide genetic polymorphic sites (SNPs, Single Nucleotide Polymorphisms) or insertion-deletion sites ...

AI Technical Summary

Problems solved by technology

This results in small fragment DNA sequence contamination, inaccurate fragment selection range, and poor distribution uniformity, which ultimately leads to poor consistency of target sequencing regions between samples.

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