A method for producing canine parvovirus monoclonal antibody by high-density culture

Abstract

The invention belongs to the technical field of canine parvovirus monoclonal antibody, and specifically relates to a method for producing canine parvovirus monoclonal antibody by means of high-density culture; the bioreactor includes a storage chamber and a reaction chamber; the upper surface of the reaction chamber A storage bin is fixedly connected through a slant plate; the storage bin includes a liquid storage tank and a liquid collection tank; a support plate is provided on the inner wall of the storage bin, and the support plate is an arc design; a liquid storage cover is sleeved above the liquid storage tank The outer surface of the liquid storage tank is fixedly connected with a liquid collection tank; the inner wall of the liquid storage tank is provided with uniformly arranged first through holes; the inner wall of each of the first through holes is fixedly connected with a control valve; The invention is mainly used to solve the problem of the existing bioreactor culture hybridoma cell production technology of canine parvovirus monoclonal antibody, the cultured cell density is low, the cultured antibody titer is low, the product needs to be concentrated and serum needs to be added during the culture, It complicates the downstream process and increases the risk of side reactions.

Description

technical field [0001] The invention belongs to the technical field of canine parvovirus monoclonal antibodies, in particular to a method for producing canine parvovirus monoclonal antibodies by means of high-density culture. Background technique [0002] Canine parvovirus disease (CPVD) is a viral infectious disease caused by canine parvovirus (CPV) infection. Vomiting, bloody stool, and a large decrease in white blood cells; one is non-suppurative myocarditis type, and the sick dog will die suddenly. This disease transmission speed is fast, and morbidity rate is high, and the mortality rate of puppies is as high as 70%, and the adult dog mortality rate is relatively low but the phenomenon of recessive infection is ubiquitous. In 1978, Kelly et al. first isolated CPV from dogs suffering from hemorrhagic enteritis. Subsequently, many countries and regions in the world reported the prevalence of this disease one after another. In 1982, Liang Shizhe and others first reported...

AI Technical Summary

Problems solved by technology

[0007] In order to make up for the deficiencies in the prior art, the present invention proposes a method for producing canine parvovirus monoclonal antibody using a high-density culture method. The technology of cloning antibody cultured cells has a low density, and the titer of cultured antibodies is low. The product needs to be concentrated and serum needs to be added during the culture, which complicates the downstream process and increases the risk of side effects

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