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Method for inducing type 2 diabetes animal model through low-temperature and dietary rhythm regulation and control

A type 2 diabetes and animal model technology, applied in the field of biomedicine, can solve the problems of unrevealed effects, differences in type 2 diabetes, etc., and achieve the effect of easy operation and simple construction method

Active Publication Date: 2020-08-21
贵州中医药大学
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  • Claims
  • Application Information

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Problems solved by technology

However, the production methods of these animal models are significantly different from the pathogenesis of human type 2 diabetes, especially the role of lifestyle and behavioral changes in the process of type 2 diabetes cannot be revealed

Method used

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  • Method for inducing type 2 diabetes animal model through low-temperature and dietary rhythm regulation and control
  • Method for inducing type 2 diabetes animal model through low-temperature and dietary rhythm regulation and control
  • Method for inducing type 2 diabetes animal model through low-temperature and dietary rhythm regulation and control

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Effect test

Embodiment 1

[0023] Example 1: Effects of changes in ambient temperature and circadian dietary rhythm on body weight and components of glucose and lipid metabolism in rats.

[0024] 1. Induction of type 2 diabetes animal model

[0025] 4-week-old 150g-200g male SD rats were selected. 12 hours of natural light per day, adaptive feeding for 1 week. Different temperatures (high temperature 30°C, suitable temperature 25°C, low temperature 16°C), time-limited diet (daytime, night), high-fat diet affect the dietary rhythm and structure of the rats, and set up a high-temperature normal diet group with a daytime restricted diet and a high-temperature normal diet group. Food restriction group at night, food restriction group with normal temperature-appropriate feed during the day, food restriction group with normal temperature-appropriate feed at night, food restriction group with normal low temperature feed during the day, food restriction group with normal low temperature feed at night, high-fat...

Embodiment 2

[0029] Example 2: Effects of low temperature environment and circadian dietary rhythm on intestinal microbial diversity in rats.

[0030] Microbial diversity sequencing technology was used to analyze the effects of low temperature environment and circadian dietary rhythm on the intestinal microbial diversity of rats. The results showed that low temperature environment can significantly reduce the intestinal microbial abundance of rats, and the microbial abundance of rats in the low temperature nighttime food restriction group minimum (see image 3 , * 0.01 Figure 4 , the Y axis represents the species name at a certain taxonomic level, the X axis represents the average relative abundance of species in different groups, and the columns with different numbers represent different groups; the far right is the P value, * 0.01 < P ≤ 0.05, **0.001 < P ≤ 0.01), suggesting that the low temperature environment may promote the colonization of Firmicutes microorganisms.

[0031] Results:...

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Abstract

The invention discloses a method for inducing type 2 diabetes animal model through low-temperature and dietary rhythm regulation and control. By combining the regulation and control of a low-temperature environment with the change of the dietary rhythm of a feeding feed or independently through the change of a dietary rhythm of the feeding feed, or through only the change of the dietary rhythm ofthe feeding feed, the animal is induced to form type 2 diabetes animal model with weight gain, hyperlipidemia, hyperglycemia, hyperinsulinemia and insulin resistance as main markers. Experimental research results show that SD rats can be induced to form main markers of type 2 diabetes such as weight gain, hyperlipidemia, hyperglycemia, hyperinsulinemia and insulin resistance by low-temperature environment and night time-limited diet, the diversity of intestinal microorganisms can be remarkably reduced, the morbidity mode is close to that of human type 2 diabetes, and the constructed diabetes animal model can be used for studying a morbidity mechanism of type 2 diabetes, searching related targets of early diagnosis, and researching and developing treatment drugs and prevention and treatmentmethods.

Description

technical field [0001] The invention relates to a method for constructing an animal model of type 2 diabetes, in particular to a method for rapidly inducing an animal model of type 2 diabetes through regulation and control of a low temperature environment and a circadian dietary rhythm, and belongs to the technical field of biomedicine. Background technique [0002] Diabetes has become one of the most serious public health problems in the world in the 21st century, most of which are type 2 diabetes. Type 2 diabetes, also known as non-insulin-dependent diabetes mellitus, is an endocrine disorder characterized by hyperglycemia, which is caused by relatively insufficient insulin secretion or decreased sensitivity of target cells to insulin, causing sugar, protein, fat, and secondary water and electrolyte metabolism disorders. - Metabolic diseases. The main clinical manifestations are "three excesses and one deficiency". "Three excesses" refer to polyuria, polydipsia, and hyper...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008A01K2207/25A01K2207/35A01K2227/105A01K2227/106A01K2227/107A01K2267/0362
Inventor 李军蒲翔陈云志王庆学柴艺汇刘晓清
Owner 贵州中医药大学
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