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Method for separating and purifying total flavonoids and total saponins from fibrous roots of ophiopogon japonicus and simultaneously removing paclobutrazol

A technology for separation and purification of total flavonoids, applied in the field of separation and purification of total saponins and total flavonoids, can solve the problems of complex operation process, low recovery rate, large loss, etc., and achieve the effect of simple process flow, less loss and quick operation

Pending Publication Date: 2020-08-21
四川莱琪尔生物科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The object of the present invention is to: provide a kind of method that separates and purifies total flavonoids and total saponins from Radix Ophiopogon japonicus fibrous root and simultaneously removes paclobutrazol, solves the problem of complex operating process, low purity of purified total saponins and total flavonoids in the prior art, and recovery The problem of low yield and large loss, the method of separating and purifying total flavonoids and total saponins from Ophiopogon japonicus fibrous roots and removing paclobutrazol at the same time in this application, compared with extracting extract, the purity of saponins in refined products is increased from 16% to 47% , the purity of flavonoids increased from 3.8% to 12%; the recovery rate of saponins was 86.49%, the recovery rate of flavonoids was 87.89%, and the loss was less; in addition, compared with the original medicinal materials, the removal rate of paclobutrazol was 85%. The process flow of this method is simple and easy to operate. fast

Method used

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  • Method for separating and purifying total flavonoids and total saponins from fibrous roots of ophiopogon japonicus and simultaneously removing paclobutrazol
  • Method for separating and purifying total flavonoids and total saponins from fibrous roots of ophiopogon japonicus and simultaneously removing paclobutrazol
  • Method for separating and purifying total flavonoids and total saponins from fibrous roots of ophiopogon japonicus and simultaneously removing paclobutrazol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Resin screening: Take an appropriate amount of the extract, dissolve it with water, weigh each resin (equivalent to 0.1g dry weight) into an Erlenmeyer flask, add 30mL sample solution, and shake it in a constant temperature shaker at 25°C and 150r / min for 24h. Suction filtration, determine the content of saponins and flavonoids in the remaining adsorption solution, and calculate the adsorption rate; then add 30mL of 95% ethanol to each conical flask, shake in a constant temperature shaker at 25°C, 150r / min for 12h, and filter with suction to determine the saponin in the desorption solution , Flavonoid content, calculate the desorption rate; the calculation result is as figure 1 Shown, according to figure 1 The displayed results show that: D101 macroporous resin is the most preferred resin.

Embodiment 2

[0052] Determine the most preferred adsorption temperature: Take 5 portions of the best resin that has been processed, equivalent to 2g of dry weight) Place them in a 500mL Erlenmeyer flask, add 200mL of sample solution, and perform static adsorption at 25℃, 35℃, and 45℃ , Calculate its adsorption rate and judge the influence of temperature on static adsorption, such as Figure 2-4 And shown in Table 1.

[0053] among them figure 2 It is the adsorption isotherm of D101 macroporous resin to the total saponins and total flavonoids in Ophiopogon japonicus fibrous roots under different temperature conditions. According to the results shown in the curve, the adsorption capacity is highest when the adsorption temperature is 25℃, and the lowest at 45℃, indicating that low temperature is beneficial to adsorption .

[0054] among them image 3 Fitting curve diagram for Langmuir isotherm adsorption equation, Figure 4 Fitting curve diagram for Freundlich isotherm adsorption equation, Langmu...

Embodiment 3

[0060] Study the effect of solution concentration, sample flow rate, sample pH on dynamic adsorption, such as Figure 5-7 As shown, take 5 parts each of the best type of resin that has been screened (equivalent to 8g dry weight), and pack them into the column; prepare sample solutions of Ophiopogon japonicus fibrous roots with different concentrations, and the column flow rate requirement is 2mL / min. Add total flavonoids and The sample solution of Ophiopogon japonicus fibrous root with a total saponin concentration of (0.198, 1.48 mg / mL; 0.396, 2.96 mg / mL; 0.594, 4.44 mg / mL; 0.792, 5.92 mg / mL; 0.99, 7.44 mg / mL) was used for dynamic adsorption experiments , Collect, measure the absorbance, calculate the adsorption rate, such as Figure 5 As shown; take 5 pretreated best type of resin (equivalent to 8g dry weight), packed into a column, each add the total flavonoids and total saponin concentration (0.198, 1.48mg / mL) Ophiopogon japonicus root sample solution; Adsorb at the speed of...

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Abstract

The invention relates to the technical field of separation and purification of total flavonoids and total saponins and provides a method for separating and purifying total flavonoids and total saponins from fibrous roots of ophiopogon japonicus and simultaneously removing paclobutrazol. The method specifically comprises the steps of: radix ophiopogonis fibrous root extraction, resin pretreatment,and macroporous resin adsorption and elution; wherein the macroporous resin adsorption and elution comprise the steps of column packing, sample loading, cleaning, elution and purification, wherein theliquid feeding concentration, liquid feeding speed, liquid feeding PH and the like are controlled. According to the method, the problems that in the prior art, the operation process is complex, the purity of purified total saponins and total flavonoids is low, the recovery rate of the saponins and the flavonoids is low, and losses are large are solved, and compared with extract extraction, the method has the advantages that the purity of the saponins in refined products is increased to 47% from 16%, and the purity of the flavonoids is increased to 12% from 3.8%; the recovery rate of saponin is 86.49%, the recovery rate of flavone is 87.89%, and the loss is low; compared with raw medicinal materials, the paclobutrazol removal rate is 85%, and the method is simple in technological process and rapid to operate.

Description

Technical field [0001] The invention relates to the technical field of separation and purification of total flavonoids and total saponins. It is used for separating and purifying total flavonoids and total saponins from Ophiopogon japonicus and removing paclobutrazol at the same time, and specifically relates to a separation and purification of total flavonoids and total saponins from Ophiopogon japonicus And the method of removing paclobutrazol at the same time. Background technique [0002] Ophiopogon is the dried root tuber of Liliaceae plant Ophiopohonjaponicus (Lf) Ker-GawL., originally published in "Shen Nong's Materia Medica". It has the effects of nourishing yin, nourishing body fluid, moisturizing the lungs and clearing the heart. Ophiopogon japonicus, sore throat and dry intestinal constipation; Ophiopogon japonicus contains chemical components such as steroidal saponins, high isoflavones, sterols and polysaccharides, of which the first two are the main active ingredien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8968A61K125/00
CPCA61K36/8968A61K2236/33A61K2236/39A61K2236/55
Inventor 黄毅金虹李沛霞刘晓梅何礼
Owner 四川莱琪尔生物科技有限责任公司
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