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Chemical mutagenic agent for corn genetic breeding

A chemical mutagen, genetic breeding technology, applied in the direction of chemicals, applications, and biocides for biological control, can solve the problem of single mutagen, low chromosome aberration rate, and low physiological damage of corn M1 Obvious problems, to achieve the effect of easy operation and simple treatment method

Active Publication Date: 2020-08-25
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Application Information

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Problems solved by technology

There are few research reports on the treatment of corn seeds with chemical agents, and all of them use EMS-paraffin oil to treat inbred line pollen, and the method and mutagen are relatively simple.
[0004] Pingyangmycin PYM is an antibiotic, and PYM is a new mutagen discovered by Chinese researchers, which is similar to the mutagenic characteristics of EMS; however, previous studies have found that the physiological damage of maize M1 is not obvious when treated with PYM alone. Chromosomal aberration rate is not too high

Method used

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  • Chemical mutagenic agent for corn genetic breeding
  • Chemical mutagenic agent for corn genetic breeding
  • Chemical mutagenic agent for corn genetic breeding

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Embodiment Construction

[0013] Test materials: Jingkenuo 2000; purchased from Corn Research Center, Beijing Academy of Agriculture and Forestry Sciences

[0014] PYM (pingyangmycin), EMS (ethyl methanesulfonate), MNU (N-methyl-N-nitrosourea); commercially available.

[0015] Test method: chemical mutagens seed treatment (refer to An Xueli, etc.). Select healthy corn seeds, rinse them with water, and sterilize them in 5% sodium hypochlorite solution for 0.5h. After disinfection, they are pre-soaked in distilled water at room temperature for 24h. Absorbent paper absorbs the surface moisture and uses the following chemical mutagens in different concentrations. Treatment, the ratio of the medicinal solution to the seeds is 1 seed / ml, treated for 8 hours, washed with tap water for 4 hours, dried the seeds, and sown in a sand basin. Observe the germination potential, germination rate, and growth height of seedlings at the three-leaf stage of M1 , Root length, root number. Note: All mutagens are diluted with 0...

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Abstract

The invention provides a chemical mutagenic agent for corn genetic breeding. The chemical mutagenic agent is a combination of pingyangmycin and N-methyl-N-nitrosourea; the concentration of the pingyangmycin is 20 ppm; and the concentration of the N-methyl-N-nitrosourea is 0.2%. The chemical mutagenic agent can be used for corn genetic breeding.

Description

Technical field [0001] The invention belongs to the field of agricultural genetic breeding, and specifically relates to a chemical mutagen for maize genetic breeding. Background technique [0002] Corn is the third largest food crop in my country, second only to wheat and rice, and has important economic value. With the further in-depth research and utilization of corn heterosis, the requirements for breeding materials are getting higher and higher. Creating breeding materials with a rich genetic basis is an urgent problem for breeders to solve. Maize chemical mutation breeding is a modern biological technology that uses chemical mutagens to process different parts of maize to cause mutations, and to study, screen and identify mutants. In artificial mutation breeding, it has the advantages of low cost, convenient use, simple operation and specific mutagenesis. Its application in breeding can increase the mutation frequency, create rich types of mutant materials, and effectively ...

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Application Information

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IPC IPC(8): A01N41/04A01N47/28A01N43/16A01P21/00A01H1/06
CPCA01N41/04A01N43/16A01N47/28A01H1/06
Inventor 陈曙金辉赵秋芳
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI