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A microfluidic chip for quantitative detection of bacteria in biological samples and using method

A microfluidic chip and biological sample technology, applied in the direction of microorganism-based methods, methods of supporting/immobilizing microorganisms, sterilization methods, etc. Long detection time, cumbersome steps and other problems, to achieve the effect of fast analysis speed, low reagent consumption and simple operation process

Active Publication Date: 2021-09-17
上海邦先医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional method of detecting bacteria in biological samples has problems such as cumbersome steps, long detection time, and the need for professional operation, which is difficult to meet the needs of on-site, rapid, micro-quantity, and portable detection of additives

Method used

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  • A microfluidic chip for quantitative detection of bacteria in biological samples and using method
  • A microfluidic chip for quantitative detection of bacteria in biological samples and using method
  • A microfluidic chip for quantitative detection of bacteria in biological samples and using method

Examples

Experimental program
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Embodiment 1

[0076] This embodiment takes figure 1 The orientation is defined in which the opening of the sample loading area faces upwards and the opening of the waste liquid area faces downwards.

[0077] Such as figure 1 and 2 As shown, a microfluidic chip for quantitative detection of bacteria in biological samples, which includes a sample loading area 111 for adding biological samples, communicates with the sample loading area 111 through a first flow channel 112 and is provided with a first filter The first filter zone 113 of the membrane 114, the first filter zone 117 that communicates with the first filter zone 113 through the second flow channel 115 and is provided with the first filter membrane 116, and the first filter zone 117 through the third flow channel 118 The second filter area 124 that is connected and provided with the second filter membrane 123, the detection area 130 that communicates with the second filter area 124 through the fourth flow channel 129, and the sec...

Embodiment 2

[0085] This embodiment takes Figure 7 The orientation is defined, where Figure 7 The front side is up.

[0086] Such as Figure 7 The structure of the shown microfluidic chip for quantitative detection of bacteria in biological samples is basically the same as that of Example 1, and the differences will be described in detail below.

[0087] The sample loading area includes a sample chamber 212 and a sample feeding port 211 connected to the sample chamber 212 , through such arrangement, it is possible to prevent the sample from overflowing due to too fast sample feeding.

[0088] There is only one first filter area 214, and two first filter membranes distributed along the vertical direction and arranged in parallel are arranged in the first filter area 214, wherein the pore size of the first filter membrane located upstream of the biological sample flow is larger than that of the downstream filter membrane. The pore size of the second filter membrane. Since there is only...

Embodiment 3

[0093] This embodiment takes Figure 8 The orientation is defined with the opening of the sample application area facing upwards.

[0094] The structure of this embodiment is basically the same as that of Embodiment 1, and the differences will be described in detail below.

[0095] Such as Figure 8 to Figure 14As shown, a microfluidic chip for the quantitative detection of bacteria in biological samples, which includes a sample loading area for adding biological samples, a first filter membrane that communicates with the sample loading area through a flow channel and is provided with The filter area, the second filter area communicated with the first filter area through the flow channel and provided with the second filter membrane, the detection area communicated with the second filter area, communicated with the second filter area through the flow channel and used for The first reagent area for adding the reagent for eluting bacteria from the second filter membrane, the se...

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Abstract

The invention relates to a microfluidic chip for the quantitative detection of bacteria in biological samples, comprising a sample loading area for adding the biological sample, a device connected to the sample loading area and provided with a first filter membrane The first filter area, the second filter area communicated with the first filter area and provided with the second filter membrane, the detection area communicated with the second filter area, and the second filter area The first reagent area communicates with the first reagent area for adding the reagent used to elute the bacteria from the second filter membrane, and the second reagent area communicates with the detection area. The microfluidic chip of the present invention has low reagent consumption and fast analysis speed, and also has the advantages of simple operation process and easy integration. The flow of biological samples on the chip is controllable, and the closed environment ensures that the inside of the chip is not polluted. , especially suitable for rapid detection of pathogenic microorganisms.

Description

technical field [0001] The invention specifically relates to a microfluidic chip for quantitative detection of bacteria in biological samples and a method for using it. Background technique [0002] The traditional method of detecting bacteria in biological samples has problems such as cumbersome steps, long detection time, and the need for professional operation, which is difficult to meet the needs of on-site, rapid, micro-quantity, and portable detection of additives. The microfluidic chip is mainly characterized by the manipulation of fluid in the micron-scale space. The chip system can integrate basic operating units such as sample biochemical reaction, separation, and detection. The microchannel forms a network, and the controllable fluid runs through the entire system, thereby realizing Various functions for routine laboratories. [0003] Combining microfluidic chip technology with pathogenic microorganism detection technology, the development of microfluidic chip th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00C12M1/34C12M1/12C12M1/00C12Q1/70C12Q1/06C12R1/92
CPCB01L3/5027B01L2300/0809B01L2300/12C12Q1/06
Inventor 颜菁申炳阳刘文佳王磊
Owner 上海邦先医疗科技有限公司