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Application of UL16 protein in preparation of related drugs for promoting mitochondrial functions of cells

A mitochondrial and protein technology, applied in the field of protein screening and application mechanism, can solve problems affecting stem cell proliferation, differentiation potential and survival, premature aging of somatic cells and mice, etc.

Pending Publication Date: 2020-08-28
广州溯原生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the downregulation of mitochondrial oxidative phosphorylation can cause premature aging in somatic cells and mice; more interestingly, many researchers have recently found that mitochondria and energy metabolism in adult / embryonic stem cells (ESCs) The function of normal stem cells may affect the proliferation, differentiation potential and survival period of normal stem cells, etc.

Method used

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  • Application of UL16 protein in preparation of related drugs for promoting mitochondrial functions of cells
  • Application of UL16 protein in preparation of related drugs for promoting mitochondrial functions of cells
  • Application of UL16 protein in preparation of related drugs for promoting mitochondrial functions of cells

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Experimental program
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Effect test

Embodiment 1

[0019] 1. Experimental Methods and Materials

[0020] The UL16 expression plasmid pET-31b(+)-UL16 of HSV-1, the expression vector pET-31b(+) was purchased from Invitrogen Company, and the plasmid pFLAG-CMV-2 was purchased from Sigma Company. Herpes simplex virus F strain (HSV-1 F), strain DH5α receptor bacteria and cells are preserved in our laboratory.

[0021] Primer design and synthesis

[0022] According to the sequence of HSV-1 UL16 gene queried on NCBI, primers UL16-for and UL16-rev were designed by Primers5.0 software for the amplification of HSV-1 UL16, and the primers were synthesized by Shanghai Sangong Company.

[0023] Table 1-1 Construction of primers used

[0024]

[0025] 1.2 Virus amplification and template preparation

[0026] A single layer of HEK293T cells was taken for HSV-1 virus culture. When about 75% of the cells were pathological, the virus liquid was collected and processed, and the HSV-1 genome was finally obtained and used as a template for ta...

Embodiment 2

[0083] Example 2 pET-31b(+)-UL16 Transfected Cells and Study on Intracellular Function

[0084] 1. Experimental materials

[0085] HEK293T cells were also cryopreserved by our laboratory.

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Abstract

The invention discloses application of UL16 protein in preparation of a small molecular medicine for promoting in-vitro differentiation of embryonic stem cells, and application of stem cell transplantation in treatment of nervous system degenerative diseases. According to the invention, by construction of UL16 plasmids to transfect host cells, the fact that UL16 can promote aerobic oxidation and oxidative phosphorylation of glucose and activate the cell productivity pathway so as to increase the intracellular ATP content and improve mitochondrial functions is found. Finally, by construction ofUL16 expression plasmids to transfect mouse embryonic stem cells, mitochondrial function state enhancement and energy metabolism enhancement are found, and stem cell differentiation can be promoted.Clinical treatment of cells in a differentiation defect state and cells with a low differentiation rate is realized; and the UL16 protein is used for treating related diseases or pathological and physiological processes of mitochondrial dysfunction or hypofunction of the cells.

Description

technical field [0001] The field of protein screening and application mechanism of the present invention, more specifically, the present invention relates to a protein UL16 that has the function of enhancing the functional state of mitochondria and increasing the differentiation efficiency of stem cells and its application. Background technique [0002] Embryonic stem cells (ESCs) provide seed cell sources for the cell replacement therapy of various metabolic liver diseases due to their ability of self-renewal, high proliferation and multi-directional differentiation, making them a research hotspot, but their induction The low efficiency of differentiation has been a key problem that is difficult to solve. In vitro, mouse cells can be directed to differentiate into almost all types of adult cells; human cells can also be successfully differentiated into neurons, liver cells, endothelial cells, cardiomyocytes, pancreatic cells and hematopoietic cells and other types of cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/03C12N15/38C12N15/70A61K35/545A61P39/06A61P3/00A61P35/00
CPCC07K14/005C12N15/70A61K35/545A61P39/06A61P3/00A61P35/00C12N2710/16722
Inventor 孙晗笑张倩利时雨
Owner 广州溯原生物科技股份有限公司
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