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Medicine for preventing and/or treating alcoholic intestinal injury

A technology for alcoholic liver injury and intestinal injury, applied in the field of microbiology and medicine, can solve the problems of slow effect and low safety, and achieve the effect of improving pathological damage and high safety

Active Publication Date: 2020-09-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs all have defects such as slow effect and low safety, and have certain limitations in clinical efficacy.

Method used

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  • Medicine for preventing and/or treating alcoholic intestinal injury
  • Medicine for preventing and/or treating alcoholic intestinal injury
  • Medicine for preventing and/or treating alcoholic intestinal injury

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0052] The preparation method of the lactobacillus suspension involved in the following examples is as follows:

[0053] Streak the Lactobacillus on the MRS solid medium and culture it at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, culture it at 37°C for 18 hours for activation, and activate two generations continuously to obtain Activation solution; the activation solution was inoculated in MRS liquid medium at an inoculum size of 2% (v / v), and cultured at 37°C for 18 hours to obtain a bacterial solution; the bacterial solution was centrifuged at 4°C and 6000g for 10 minutes, The Lactobacillus cells were obtained; the Lactobacillus cells were washed 3 times with normal saline and then resuspended in a skimmed milk powder solution with a concentration of 120g / L to a bacterial concentration of 1×10 9 CFU / mL to obtain a bacterial suspension, and store the bacterial suspension at -80°C until use.

Embodiment 1

[0054] Example 1: Screening and Identification of Lactobacillus helveticus CCFM1121

[0055] 1. Screening

[0056] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After pretreatment, the sample is stored in a -80°C refrigerator in about 30% glycerin. .9% normal saline for gradient dilution, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick single colonies and transfer to the liquid The MRS liquid medium was enriched and preserved in 30% glycerol to obtain the strain CCFM1121 (the original serial number of the strain was M2-09-R02-S146).

[0057] 2. Identification

[0058] The genome of CCFM1121 was extracted, and the 16SrDNA of CCFM1121 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ ID NO.1. ...

Embodiment 2

[0059] Embodiment 2: the cultivation of Lactobacillus helveticus CCFM1121

[0060] After inserting Lactobacillus helveticus CCFM1121 into the MRS solid medium and culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope. It was found that the colonies were milky white semicircular protrusions with smooth and moist surfaces and edges tidy.

[0061] Lactobacillus helveticus CCFM1121 was inserted into MRS liquid medium and cultured at 37°C for 48 hours. During the cultivation process, the pH of the culture solution was measured by a pH meter at intervals. It was found that Lactobacillus helveticus CCFM1121 produced acid during the cultivation process.

[0062] Insert Lactobacillus helveticus CCFM1121 into the MRS liquid medium and culture at 10-50°C for 48 hours. During the cultivation process, measure the OD of the culture solution with a microplate reader at intervals 600 , it was found that Lactobacillus helveticus CCFM1121 grew best at 30-37...

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Abstract

The invention discloses a medicine for preventing and / or treating alcoholic intestinal injury, and belongs to the technical field of microorganisms and medicines. The invention provides a medicine forpreventing and / or treating alcoholic intestinal injury or alcoholic liver injury. The effective component of the medicine is lactobacillus helveticus CCFM1121, and the effective component of the medicine is lactobacillus helveticus CCFM1121. The lactobacillus helveticus CCFM1121 can effectively relieve alcoholic intestinal injury and alcoholic liver injury, and is specifically embodied in that (1) the mRNA expression quantity of intestinal tight junction protein of an alcohol modeling mouse is remarkably increased; (2) the content of endotoxin (LPS) in serum of alcohol modeling mice is remarkably reduced; (3) the activity of glutamic-pyruvic transaminase (ALT) and glutamic oxalacetic transaminase (AST) in serum of alcohol modeling mice is remarkably reduced; and (4) the pathological injury of the liver tissue of the alcohol modeling mouse is obviously improved.

Description

technical field [0001] The invention relates to a medicine for preventing and / or treating alcoholic intestinal injury, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the per capita consumption of alcohol in my country has increased significantly, and the incidence of alcohol-related organ damage diseases has also increased year by year. [0003] Intestinal tract is one of the important target organs of alcohol injury. Alcoholic intestinal injury will cause the dysfunction of human intestinal mucosal epithelial cells, and then change the permeability of human small intestine, which will eventually lead to high resistance to pathogenic factors such as endotoxin and bacteria in human small intestine. permeability. The high permeability of the small intestine to pathogenic factors such as endotoxin and bacteria can lead to fever, ...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P1/00A61P1/16
CPCA61K35/747A61P1/00A61P1/16
Inventor 翟齐啸陈卫朱诗雅于雷雷田丰伟赵建新张灏
Owner JIANGNAN UNIV
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