Medicine for preventing and/or treating alcoholic intestinal injury
A technology for alcoholic liver injury and intestinal injury, applied in the field of microbiology and medicine, can solve the problems of slow effect and low safety, and achieve the effect of improving pathological damage and high safety
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preparation example Construction
[0052] The preparation method of the lactobacillus suspension involved in the following examples is as follows:
[0053] Streak the Lactobacillus on the MRS solid medium and culture it at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, culture it at 37°C for 18 hours for activation, and activate two generations continuously to obtain Activation solution; the activation solution was inoculated in MRS liquid medium at an inoculum size of 2% (v / v), and cultured at 37°C for 18 hours to obtain a bacterial solution; the bacterial solution was centrifuged at 4°C and 6000g for 10 minutes, The Lactobacillus cells were obtained; the Lactobacillus cells were washed 3 times with normal saline and then resuspended in a skimmed milk powder solution with a concentration of 120g / L to a bacterial concentration of 1×10 9 CFU / mL to obtain a bacterial suspension, and store the bacterial suspension at -80°C until use.
Embodiment 1
[0054] Example 1: Screening and Identification of Lactobacillus helveticus CCFM1121
[0055] 1. Screening
[0056] Take fresh feces from healthy people in Kunshan City, Jiangsu Province as the sample. After pretreatment, the sample is stored in a -80°C refrigerator in about 30% glycerin. .9% normal saline for gradient dilution, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick single colonies and transfer to the liquid The MRS liquid medium was enriched and preserved in 30% glycerol to obtain the strain CCFM1121 (the original serial number of the strain was M2-09-R02-S146).
[0057] 2. Identification
[0058] The genome of CCFM1121 was extracted, and the 16SrDNA of CCFM1121 was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the bacterial strain was shown in SEQ ID NO.1. ...
Embodiment 2
[0059] Embodiment 2: the cultivation of Lactobacillus helveticus CCFM1121
[0060] After inserting Lactobacillus helveticus CCFM1121 into the MRS solid medium and culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope. It was found that the colonies were milky white semicircular protrusions with smooth and moist surfaces and edges tidy.
[0061] Lactobacillus helveticus CCFM1121 was inserted into MRS liquid medium and cultured at 37°C for 48 hours. During the cultivation process, the pH of the culture solution was measured by a pH meter at intervals. It was found that Lactobacillus helveticus CCFM1121 produced acid during the cultivation process.
[0062] Insert Lactobacillus helveticus CCFM1121 into the MRS liquid medium and culture at 10-50°C for 48 hours. During the cultivation process, measure the OD of the culture solution with a microplate reader at intervals 600 , it was found that Lactobacillus helveticus CCFM1121 grew best at 30-37...
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