Automatic IVF culture and time difference analysis device and matched culture dish

Abstract

The invention discloses an automatic IVF culture and time difference analysis device and a matched culture dish. The device is composed of a main device, a matched gas-liquid flow device and an integrated controller; and the main device comprises a heat insulation sealing cover, a sample adding device, a culture dish bearing device, and a CO2 concentration, O2 concentration, humidity and temperature monitoring and adjusting device. The culture dish is provided with an oil seal cavity groove, a small culture chamber, a culture dish culture solution feeding hole and a culture dish waste discharge hole, and a culture solution buffer cavity communicated with the culture dish culture solution feeding hole and the injection port of the small culture chamber and a waste liquid buffer cavity communicated with the culture dish waste discharge hole and the waste discharge hole of the small culture chamber are arranged below the oil seal cavity groove. Relative constancy of a culture environmentcan be maintained, a spiral flow liquid changing technology can guarantee the removing effect of sperm cells, cumulus cells and metabolic waste, concentration gradient changing of all the culture solutions is achieved, external stimulation to fertilized eggs is reduced to the maximum extent, and the fertilization rate and the blastocyst cultivation rate are effectively increased.

Description

technical field [0001] The invention belongs to the field of biomedical equipment, and in particular relates to an automated IVF (in vitro fertilization, also known as in vitro fertilization, also known as in vitro fertilization, also known as test-tube baby) culturing and time difference analysis device and a matching petri dish controlled by a computer or PLC, which are used for automatically culturing human or animal fertilized eggs and displaying them in real time. Micro-observation evaluates the culture process. Background technique [0002] In recent years, due to factors such as the liberalization of the national two-child policy and environmental impact, the number of couples who need to undergo IVF has skyrocketed, and most of them (about 60%) need to undergo a first-generation IVF test. During the IVF (in vitro fertilization, also known as in vitro fertilization) experiment, each woman usually takes about ten eggs for stripping cumulus cells, making droplets, oil s...

AI Technical Summary

Problems solved by technology

[0003] At present, the tedious production of droplets and the replacement of culture medium still need to be done manually, and the external stimulation is relatively large during the culture process. According to statistics, the first-generation test-tube baby experiment has only a 70% fertilization rate and a 40% blastocyst culture rate. Greatly limit the development of IVF industry

Moreover, the strict verification system of the IVF laboratory also limits the number of technicians in the IVF laboratory to a certain extent, which indirectly leads to the low efficiency and high cost of IVF experiments.

[0004] In addition, in the process of culturing fertilized eggs, it is necessary to combine microscopic imaging and morphological analysis techniques for time difference analysis. During this process, it is also necessary to ensure that the fertilized eggs are in a relatively constant CO 2 Concentration, O 2 Concentration, temperature, humidity and a relatively light-proof environment to reduce the stimulation of external factors to fertilized eggs, while the existing experimental equipment is difficult to ensure the environmental requirements for the fertilized egg culture and analysis process

Images