A strain of Saccharomyces cerevisiae and its application in the preparation of fermented food
A technology of Saccharomyces cerevisiae and fermented food, applied in the field of microorganisms, can solve the problems of long proofing time, short proofing time of bread, hidden dangers of baked food food safety, etc., and achieve the effect of good texture and storage performance, and strong proofing ability
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Embodiment 1
[0041] Example 1: Screening and Identification of Saccharomyces cerevisiae LBBE
[0042] 1. Screening
[0043] Taking the natural grape fermented liquid collected from Fujian Maidu Food Development Co., Ltd. as a sample, take 10 mL of the natural grape fermented liquid sample and add it to the YPD liquid medium. After cultivating at 30 °C and 220 r / min for 24 hours, the culture liquid is obtained; the culture liquid Diluted 100 times and spread on YPD solid medium, cultivated at 30°C for 48 hours to obtain a single colony; use the microbial screening system Qpix420 to transfer the single colony on the YPD solid medium to a 96 deep-well plate containing YPD liquid medium, After culturing at 30°C and 220r / min for 24h, transfer to a 96-deep-well plate equipped with bacterial screening medium, continue culturing at 30°C and 220r / min for 24h to obtain bacterial liquid; measure OD with a microplate reader 600 , select OD 600Bacterial liquid greater than 0.5 was streaked and purifi...
Embodiment 2
[0046] Example 2: Domestication and Identification of Saccharomyces cerevisiae LBBE-1
[0047] 1. High sugar acclimation
[0048] The seed solution of the Saccharomyces cerevisiae (Saccharomycescerevisiae) LBBE obtained in Example 1 was inoculated to a 24-deep well plate with high glucose medium (containing 100g / L glucose) with an inoculum size of 10% (v / v), at 30°C , 220r / min cultured for 24h to obtain bacterial liquid; measure OD by microplate reader 600 , select OD 600 For the larger 3 parts of bacterial liquid, take 3 parts of bacterial liquid OD 600 The average value is recorded as the acclimatization 1d bacterial concentration; repeat the above operation, and when the measured bacterial concentration reaches the same level as that cultivated in the YPD medium, the glucose concentration is raised to a higher level (in steps of 100g / L glucose), until the bacterial strain is in It can grow normally in high glucose medium with 300g / L glucose.
[0049] During the whole hi...
Embodiment 3
[0061] Example 3: Production of Sweet Bread
[0062] The seed liquid of Saccharomyces cerevisiae (Saccharomycescerevisiae) LBBE obtained in Example 1 and the bacterium liquid of Saccharomyces cerevisiae (Saccharomyces cerevisiae) LBBE-1 obtained in Example 2 were inoculated to YPD liquid medium with an inoculum size of 10% (v / v) respectively culture medium at 30°C and 220r / min for 12h to obtain a culture solution; the culture solution was centrifuged at 6000r / min for 7min to collect bacteria.
[0063] Taking Swallow Yeast and the starting strain Saccharomyces cerevisiae LBBE as controls, weigh 1000g of high-gluten flour, 300g of sugar, 20g of salt, 200g of butter, 60g of milk powder, 200g of egg liquid, 40g of cells of domesticated Saccharomyces cerevisiae LBBE-1 and 200g of water In the noodle mixer, make dough; divide the dough into 90g dough after relaxing for 10 minutes, proof the time to double the size, exhaust the air and proof again to double the size to get the proofe...
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