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PARP15 protein and inhibitor 3-aminobenzamide compound crystal thereof and preparation method of PARP15 protein and inhibitor 3-aminobenzamide compound crystal

Inactive Publication Date: 2020-09-01
李健 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a method for successfully obtaining PARP15 protein and its inhibitor 3-aminobenzamide (3-AB) complex crystal, to solve the problem of failing to obtain PARP15 protein crystallization and lack of research on the protein in the prior art basic question

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  • PARP15 protein and inhibitor 3-aminobenzamide compound crystal thereof and preparation method of PARP15 protein and inhibitor 3-aminobenzamide compound crystal
  • PARP15 protein and inhibitor 3-aminobenzamide compound crystal thereof and preparation method of PARP15 protein and inhibitor 3-aminobenzamide compound crystal
  • PARP15 protein and inhibitor 3-aminobenzamide compound crystal thereof and preparation method of PARP15 protein and inhibitor 3-aminobenzamide compound crystal

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Embodiment Construction

[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, and the schematic embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0032] 1. Construction of PARP15 protein expression plasmid

[0033] The gene encoding human PARP15 protein was cloned into the pET-28a vector to construct an expression plasmid. This work was entrusted to Shanghai Jierui Bioengineering Co., Ltd. The size of PARP15 protein is 26kDa, and the gene sequence encoding human PARP15 protein is shown in SEQ ID NO .1 shown.

[0034] SEQ ID NO.1:

[0035] 5'--3'

[0036] GGATCCATG AAT CTG CCG GAA CAT TGG ACC GAT ATG AAT CAT CAG CTG TTTTGC ATG GTG CAG CTG GAA CCG GGC CAG AGC GAA TAC AAT ACC ATT AAA GAT AAG TTTACC CGT ACC TGC AGC AGC TAT GCG ATT GAG AAA ATT GAA CGT ATT CAG AAT GCG TTTCTG TGG CAG AGC TAT CAG GTG AAA AAA CGT CAG ATG GAT...

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Abstract

The invention discloses a PARP15 protein and an inhibitor 3-aminobenzamide compound crystal thereof and a preparation method of the PARP15 protein and the inhibitor 3-aminobenzamide compound crystal.The preparation method comprises the following steps of: mixing PARP15 protein and the inhibitor 3-aminobenzamide thereof in proportion; and crystallizing the obtained mixture by adopting a pendant-drop method in a gas phase diffusion method to obtain PARP15 protein and the inhibitor 3-aminobenzamide compound crystal thereof. The method has the advantages that the high-purity PARP15 protein is successfully obtained, the protein and the inhibitor 3-AB compound crystal thereof are obtained for the first time, research on the PARP15 protein can be promoted, and a molecular basis is provided for design and optimization of PARP15 protein-targeting drugs.

Description

technical field [0001] The invention relates to the technical field of protein crystallization, in particular to the PARP15 protein crystallization technology. Background technique [0002] PARP15 protein (polyadenosine diphosphate-ribose polymerase family member 15) is overexpressed in diffuse large B-cell lymphoma. Studies have reported that PARP15 protein has methylation activity. As tumor suppressor genes (TsGs) are often inactivated through somatic mutations and / or promoter methylation, PARP15 proteins have a suppressive effect on tumors. At present, there are few studies on PARP15 protein, and even less research on its biological function. However, the PARP family has a great influence on cancer, and some scholars believe that PARP15 protein is a candidate gene for cancer development and has the ability to regulate gene transcription. Furthermore, it has been reported to be involved in apoptosis. [0003] The three-dimensional structure of protein plays a very impo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/70C12R1/19
CPCC12N9/1077C12N15/70C12Y204/0203C07K2299/00
Inventor 李健张进周学兰万双燕
Owner 李健