Blood genome DNA extraction method based on 3D printed special-shaped functional body and application kit thereof

A 3D printing and extraction method technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of complex genomic DNA process, time-consuming, low throughput, etc., and achieve simple operation process and stable separation effect , the effect of fast separation speed

Pending Publication Date: 2020-09-01
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the complicated, time-consuming and low-throughput process of extracting genomic DNA from blood, the present invention provides a method for extracting genomic DNA from blood based on 3D printed special-shaped functional body

Method used

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  • Blood genome DNA extraction method based on 3D printed special-shaped functional body and application kit thereof
  • Blood genome DNA extraction method based on 3D printed special-shaped functional body and application kit thereof
  • Blood genome DNA extraction method based on 3D printed special-shaped functional body and application kit thereof

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Experimental program
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Effect test

Embodiment 1

[0057] Using photosensitive resin (PAA) as raw material, DLP digital image projection 3D printing technology is used to undergo photocuring reaction under 400-800nm ​​light, so as to obtain 3D printed special-shaped functional body with regular structure: 3D printed special-shaped functional body is similar to Umbrella structure, single 3D printing special-shaped functional body is composed of nucleic acid binding area 1 and handle area 2, nucleic acid binding area 1 is a cone, handle area 2 is a cylinder, the center of the cone bottom of nucleic acid binding area 1 and the handle area 2 A bottom connection. Specifically, it includes one monomer functional body with a single nucleic acid binding region 1, the nucleic acid binding region 1 has a width of 5mm and a height of 10mm, the outer surface of the nucleic acid binding region 1 has a thread and groove structure, and the handle region 2 has a height of 35mm. Single 3D printing special-shaped functional body such as figure...

Embodiment 2

[0061] (1) Preparation of special functional body

[0062] Using photosensitive resin (PAA) as raw material, DLP digital image projection 3D printing technology is used to undergo photocuring reaction under 400-800nm ​​light, so as to obtain 3D printed special-shaped functional body with regular structure: 3D printed special-shaped functional body is similar to Umbrella-shaped structure, the eight-unit 3D printing special-shaped functional body is composed of a nucleic acid binding area 1, a handle area 2 and a connecting area 3. The nucleic acid binding area 1 is a cone, the handle area 2 is a cylinder, and the connecting area 3 is a long strip. The central position of the cone bottom of the nucleic acid binding area 1 is connected to one bottom surface of the handle area 2, the other bottom surface of the handle area 2 is connected to the connection area 3, and eight single 3D printing special-shaped functional bodies are connected side by side on the connection area 3 to for...

Embodiment 3

[0072] Using photosensitive resin (PAA) as raw material, DLP digital image projection 3D printing technology is used to undergo photocuring reaction under 400-800nm ​​light, so as to obtain 3D printed special-shaped functional body with regular structure: 3D printed special-shaped functional body is similar to Umbrella-shaped structure, the eight-unit 3D printing special-shaped functional body is composed of a nucleic acid binding area 1, a handle area 2 and a connecting area 3. The nucleic acid binding area 1 is a cone, the handle area 2 is a cylinder, and the connecting area 3 is a long strip. The central position of the cone bottom of the nucleic acid binding area 1 is connected to one bottom surface of the handle area 2, the other bottom surface of the handle area 2 is connected to the connection area 3, and eight single 3D printing special-shaped functional bodies are connected side by side on the connection area 3 to form eight Connected 3D printing special-shaped functio...

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Abstract

The invention discloses a blood genome DNA extraction method based on a 3D printed special-shaped functional body and an application kit thereof, and belongs to the field of nucleic acid extraction and separation. According to the method, a sample is subjected to a cracking treatment by using a cracking solution, and nucleic acid combination, separation and purification are carried out by using a3D printed special-shaped functional body, the 3D printed functional body is quickly transferred in a cracking solution, a cleaning solution and an eluent in a handheld or mechanical holding mode, time-consuming and tedious steps such as liquid transferring and centrifuging are not needed, the manual labor density is low, the cost is low, the flux is high, the quality of obtained nucleic acid is high, the requirement for equipment and operation space is small, and the method has automation and high-flux downstream application prospects. Furthermore, the invention also provides an application kit of the blood genome DNA extraction method. The invention solves the problems of complex preparation of target nucleic acid, time consumption, and low flux in an existing PCR in-vitro amplificationtechnology and the method has wide application prospects in rapid detection of blood pathogens.

Description

technical field [0001] The invention belongs to the field of nucleic acid extraction and separation, and in particular relates to a blood genome DNA extraction method based on 3D printed special-shaped functional bodies and an application kit thereof. Background technique [0002] Diseases transmitted through blood often cause major infectious diseases and pose global public health security problems. The prevention of such infectious diseases is mainly based on three principles: control the source of infection, cut off the route of transmission, and protect susceptible people. The most effective means of prevention is to find the source of infection as early as possible through the detection of pathogens and cut off the transmission. High-throughput and high-sensitivity pathogen detection methods are the key issues to improve detection efficiency and accuracy. Currently commonly used pathogen detection methods include: pathogen detection, immunology detection and molecular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2523/308C12Q2521/327C12Q2521/537
Inventor 李佩佩姜晓滨李蒙航袁志杰范琦
Owner DALIAN UNIV OF TECH
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