Preservation and transportation liquid for decellularized lamellar corneas

A technology of lamellar cornea and transport fluid, which is applied in the field of preservation and transportation of medical materials, can solve the problems of unsuitable preservation of acellular lamellar cornea, achieve the effect of no cytotoxicity, small molecular weight, and increase colloid osmotic pressure

Pending Publication Date: 2020-09-22
SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing corneal preservation solution is only suitable for penetrating keratoplasty or endothelial transplantation, not suitable for the preservation of acellular lamellar cornea

Method used

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  • Preservation and transportation liquid for decellularized lamellar corneas
  • Preservation and transportation liquid for decellularized lamellar corneas
  • Preservation and transportation liquid for decellularized lamellar corneas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The storage and transportation solution of this embodiment includes the following components in weight percentage: 65% glycerin, 6% dextran with a molecular weight of 20,000 to 40,000, 0.2% N-acetylcysteine, and the balance is deionized water .

[0027] The preparation method of the acellular lamellar cornea storage transport solution in this example is: dissolving N-acetylcysteine ​​and dextran with a molecular weight of 20,000 to 40,000 in deionized water, and adjusting the pH value of the solution after mixing evenly to 7.2, then add glycerin and mix well.

[0028] The acellular lamellar cornea with a thickness of 0.49 mm is preserved by using the preservation and transportation solution of this embodiment. One month later, the thickness of the cornea was measured to be 0.48±0.08mm. The preserved cornea had good transparency, no yellowing, and maintained good thickness, toughness and elasticity, and no swelling after rehydration.

Embodiment 2

[0030] The storage and transportation solution of this embodiment includes the following components in weight percentage: 65% glycerin, 2% chondroitin sulfate, 0.5% sodium hyaluronate with a molecular weight of 200,000 to 400,000, and about 20,000 to 40,000 molecular weight. Glucoside 6%, N-acetylcysteine ​​0.2%, and deionized water as the balance.

[0031] The preparation method of the acellular lamellar cornea storage transport solution in this example is: dissolving N-acetylcysteine, chondroitin sulfate, sodium hyaluronate and dextran with a molecular weight of 20,000 to 40,000 in deionized water, adjust the pH value of the solution to 7.2 after mixing evenly, then add glycerin and mix evenly.

[0032] The acellular lamellar cornea with a thickness of 0.49mm is preserved by using the preservation and transportation solution of this embodiment. After one month, the thickness of the cornea was measured to be 0.48±0.05mm. After preservation, the cornea had good transparency, ...

Embodiment 3

[0034] The storage and transportation solution of this embodiment includes the following ingredients in weight percentage: 65% glycerin, 6% chondroitin sulfate, 0.2% N-acetylcysteine, and the balance is deionized water.

[0035] The preparation method of the acellular lamellar cornea storage transport solution in this example is as follows: dissolve N-acetylcysteine ​​and chondroitin sulfate in deionized water, mix well, adjust the pH value of the solution to 7.2, and then add glycerin and mix well .

[0036] The decellularized lamellar cornea was preserved using the preservation and transport solution of this embodiment, and the cornea after preservation for 3 months had good transparency and no yellowing phenomenon (see figure 1 ), and can maintain good thickness, toughness and elasticity, and will not swell after rehydration.

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Abstract

The invention discloses a preservation and transportation liquid for decellularized lamellar cornea, which comprises the following components in percentage by weight: 61-90% of a thickness maintainingagent, 0.1-2% of an antioxidant and the balance of a solvent, wherein the thickness maintaining agent is a mixture of glycerol and one or more of dextran, chondroitin sulfate, sodium alginate and sodium hyaluronate, and the weight percentage of glycerol in the preservation and transportation liquid is 60%-80%; the antioxidant is one or more of superoxide dismutase, melatonin, N-acetylcysteine andreduced glutathione. The decellularized lamellar cornea preservation and transportation liquid is suitable for protecting the structure and stability of biological materials such as decellularized lamellar cornea collagen and maintaining the appearance and transparency of the cornea, and meanwhile the damage to the cornea caused by irradiation sterilization can be avoided.

Description

technical field [0001] The invention relates to the technical field of preservation and transportation of medical materials, in particular to a preservation and transportation solution for decellularized lamellar cornea. Background technique [0002] At present, corneal transplantation is divided into penetrating keratoplasty, lamellar keratoplasty and corneal endothelial transplantation according to the components of the graft and surgical methods. Lamellar keratoplasty and corneal endothelial transplantation have the advantages of less incidence of rejection and fewer long-term complications. The volume is increasing year by year. Lamellar keratoplasty is very suitable for countries with insufficient sources of corneal materials because it is not limited by the material storage time limit. [0003] The composition of corneal preservation solution directly affects the quality of cornea. The existing corneal preservation solutions are only suitable for penetrating keratopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 樊杏利任珂星王二浩张勇杰
Owner SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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