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Method for producing fermentation product

A technology for fermentation products and manufacturing methods, applied in the direction of fermentation, biochemical equipment and methods, separation methods, etc., which can solve the problem that fermentation products are rarely reused

Pending Publication Date: 2020-09-22
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But, in these existing methods, thalline can be reused, but unutilized culture raw material is discarded in the recovery operation of fermentation product more often, the situation of being reused in the production of fermentation product is less few

Method used

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  • Method for producing fermentation product
  • Method for producing fermentation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] (1) Construction of Bacillus subtilis mutant strain (168_protease strain) introduced into protease gene expression mutation

[0142] Introduction of protease gene expression mutations was carried out. Using the genomic DNA of the Bacillus subtilis mutant strain RIK1140 (trpB'A'::PrrnOcatpt1 erm hisC101) (Japanese Patent No. 5847458) as a template, use the primers trpB-F and PrrnO-catsd-R described in Table 1 to amplify by PCR Contains the Shine-Dalgarno (SD) sequence in the upstream region of the chloramphenicol resistance gene from the trpB gene (Shine, J., Dalgarno, L., Proc. Natl. Acad. Sci. USA., 1974, 71: 1342 -1346) fragment (A). In addition, using the primers PrrnO-cat-erm-F and hisC-R2 described in Table 1, a fragment (B) containing the erythromycin resistance gene and the hisC gene was amplified by PCR.

[0143] Next, with reference to the records in JP-A-2002-218989, JP-A-2002-306176, JP-A-2004-122, JP-A-2004-305176, and JP-A-2006-129865, KP43 protease muta...

Embodiment 2

[0178] In the first culture of Example 1, the same operation was performed except that the culture was performed for 97 hours. In the first culture, the cell concentration (OD600 value) after 97 hours of culture was 30, in addition, the amount of residual sugar in the culture solution was 13 (g / L), and the production amount of alkaline protease was 0.27 (g / L) ). In the second culture, the cell concentration (OD600 value) after 76 hours of culture was 73, and the production amount of alkaline protease was 0.53 (g / L).

[0179] In addition, the carbon source conversion rate of alkaline protease was 0.71% with respect to the total sugar input amount in the first culture and the second culture.

Embodiment 3

[0181] In the first culture of Example 1, the same operation was performed except that the culture was performed for 122 hours. In the first culture, the cell concentration (OD600 value) after 122 hours of culture was 29, in addition, the amount of residual sugar in the culture solution was 10 (g / L), and the production amount of alkaline protease was 0.27 (g / L) ). In the second culture, the cell concentration (OD600 value) after 76 hours of culture was 68, and the production amount of alkaline protease was 0.59 (g / L).

[0182] In addition, the carbon source conversion rate of alkaline protease was 0.77% with respect to the total amount of sugar input in the first culture and the second culture.

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Abstract

Provided is a method which allows fungus and unutilized culture raw materials to be effectively used for the production of a fermentation product. Provided is a method for producing a fermentation product by culturing a microorganism, the method comprising the following steps (A)-(D): (A) a step for culturing a microorganism in a first medium; (B) a step for liquid-passing a culture liquid, whichcontains fungus after culture, culture raw materials, and a fermentation product, through an adsorption column which is filled with an adsorbent for adsorbing the fermentation product and satisfies the relationship D1<d<D2(where the size of fungus (minor diameter) is d, the pore diameter of the adsorbent is D1, and the minimum void diameter between the adsorbents is D2), for causing the fermentation product in the culture liquid to be adsorbed onto the adsorbent, and for recovering an effluent containing the fungus and the culture raw materials flowing out from the adsorbing column; (C) a stepfor bringing an eluate into contact with the adsorbent, and causing the fermentation product to be eluted; and (D) a step for culturing the microorganism in a second medium using the effluent containing the fungus and the culture raw materials that have been recovered.

Description

technical field [0001] The present invention relates to a method for producing a fermentation product using microorganisms. Background technique [0002] In recent years, techniques for producing industrially useful compounds by fermentation using microorganisms have been put into practical use. [0003] In addition to the fermentation product, unused culture materials and microbial cells are mixed together in the fermentation culture liquid after microbial cultivation. Therefore, in the operation of obtaining the fermentation product from the culture liquid, the bacteria are usually separated first, and then the fermentation product is separated. It is separated from the solution containing unused culture raw materials, and the operation of recovery is carried out. In the separation operation, centrifugation, membrane separation, adsorption separation, etc. are utilized (for example, Patent Documents 1 to 3). [0004] In addition, it has been reported that while filtering...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12N9/42C12N9/54C12N15/09
CPCC12P21/02C12N15/09C12N1/20C12Y304/23001C12Y304/21001C12Y304/21064C12Y301/03008B01D15/10C12N9/54
Inventor 木村俊介四方健一
Owner KAO CORP