The method of distant hybridization between koi and bighead carp subfamilies and the application of tetraploid koi and bighead carp

A technology of distant hybridization and tetraploidy, which is applied in applications, medical preparations containing active ingredients, fish farming, etc., can solve the problems of large difference in chromosome number, no ploid relationship, and difficult hybrid offspring, etc., to achieve Improve the hatching rate and survival rate, shorten the breeding cycle, and improve the performance of the traits

Active Publication Date: 2022-03-08
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a large difference in the number of chromosomes between koi and bighead carp, and there is no ploidy relationship, so it is difficult to obtain hybrid offspring smoothly by conventional methods

Method used

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  • The method of distant hybridization between koi and bighead carp subfamilies and the application of tetraploid koi and bighead carp
  • The method of distant hybridization between koi and bighead carp subfamilies and the application of tetraploid koi and bighead carp
  • The method of distant hybridization between koi and bighead carp subfamilies and the application of tetraploid koi and bighead carp

Examples

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Effect test

Embodiment 1

[0043] A method for distant hybridization between koi carp and bighead carp subfamily of the present invention, comprises the steps:

[0044] (1) The parent selection of distant hybridization is 3 to 4 months before the breeding period of the fish, and the female koi and the male bighead carp that are healthy, healthy, and normally developed are selected as the female parent fish and the male parent fish respectively, Move the selected broodstock out of the pond for special cultivation to avoid accidents; feed them scientifically with concentrated feed 1 to 2 months before the breeding season, and regularly stimulate the fish with running water during the period to promote the maturation of the gonads of the broodstock; normal development refers to sexual growth. The characteristics are obvious, and the body is healthy, which means that there is no disease and no physical trauma;

[0045] The water quality indicators for raising female koi are as follows: pH is 7.0 to 7.4, har...

Embodiment 2

[0064] The application of using the tetraploid golden carp and bighead hybrid fish obtained in Example 1 to cultivate triploid fish, the specific method includes the following steps: select the female diploid crucian carp with enlarged abdomen and softness as the female parent, and use a needle injection method to artificially induce labor , the injected oxytocin included chorionic gonadotropin 500 μg / kg, luteinizing hormone releasing hormone analogue 4 μg / kg, and dioconone 1 μg / kg; at the same time, a strong, well-developed, slender male tetraploid golden carp was selected The fish is the male parent, and one injection is used to artificially induce labor. The injected oxytocin includes chorionic gonadotropin 200 μg / kg and luteinizing hormone releasing hormone analogue 3 μg / kg. After the injection, the ratio of male to female fish is 1:2. Put it into the spawning pool for induced spawning, put the net and brown slices in the pool, when the eggs appear on the net or brown slice...

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Abstract

The invention discloses a method for distant hybridization between koi and bighead carp subfamilies, which comprises the following steps: using koi as the female parent and bighead carp as the male parent to artificially induce labor, and adopting a one-needle injection method to treat the parent fish of the female parent. Inject mixed oxytocin containing LRH‑A, HCG and dioconone for oxytocin injection; adopt two-needle injection method to inject mixed oxytocin containing LRH‑A and HCG for oxytocin in male parent fish, and ovum and sperm obtained after artificial oxytocin Artificial dry insemination, and then the fertilized eggs are hatched in flowing water. After successful hatching, the hybrid offspring of koi and bighead carp will be obtained. The obtained new hybrid progeny provides a new resource and foundation for the genetic breeding of freshwater fish, and the whole hybridization process is simple to operate, the breeding period is short, and the hatching rate and survival rate of the hybrid progeny are high. The invention also discloses the application of hybrid progeny tetraploid golden carp, which can obtain a large number of triploid fish with fast growth speed and strong stress resistance, and has great industrial application prospect.

Description

technical field [0001] The invention belongs to the field of freshwater fish hybridization, in particular to a method for distant hybridization between koi and bighead carp subfamilies and the application of tetraploid koi and bighead carp. Background technique [0002] Distant hybridization refers to the hybridization of kinship between species and above, and has many applications in fish genetics and breeding. Distant hybridization can integrate the exogenous genes of the parents, so that the advantages of the parents can be combined in the hybrid, and produce heterosis, which has better performance in terms of growth cycle and disease resistance. For example, the distant hybridization between koi and bream subfamily, and the distant hybridization between red crucian carp and Xiangjiang wild carp produce better hybrid offspring. However, the long-term problem faced by the field of distant hybridization is that the traits of hybrids segregate greatly and are not easy to st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A61K38/09A61P15/04A61K31/454
CPCA01K61/10A61K38/09A61K31/454A61K9/0019A61P15/04A61K2300/00Y02A40/81
Inventor 刘少军王余德姚嘉骏谭慧芳罗凯坤覃钦博张纯陶敏汤陈宸
Owner HUNAN NORMAL UNIVERSITY
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