Biofilm culturing method capable of simultaneously removing tetrahydrofuran and styrene by utilizing bio-trickling filtration process
A technology of tetrahydrofuran and film hanging method, applied in biochemical equipment and methods, chemical instruments and methods, microorganisms, etc., can solve the problems of large exhaust gas emissions, difficulty in practical application of biological methods, and high concentration, and achieve the effect of exhaust gas concentration control.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] collection of samples
[0041] The waste water in the aeration tanks of two different chemical companies was collected with sterile sampling bottles, and placed in a constant temperature incubator for overnight cultivation, and the temperature was maintained at about 35°C.
Embodiment 2
[0043] Preliminary screening of specific strains
[0044] Select 20 100mL Erlenmeyer flasks and fill them with 30mL LB medium respectively, and fill 10 100mL Erlenmeyer flasks with 30mL MM medium respectively, and put them into a high-pressure steam cooker for 30min to sterilize. Randomly extract 5 parts of each of the two water samples, divide them into the above-mentioned 10 Erlenmeyer flasks, and put them in a shaker at a temperature of 37°C and a speed of 180r / min for the first-generation culture for 12 hours; Insert 5% of the inoculum into 10 Erlenmeyer flasks used as the second-generation LB medium, and put it into a shaker under the same conditions for 3 hours; the second-generation LB medium was 15% by volume The inoculum was respectively inserted into the inorganic salt medium with tetrahydrofuran as the sole carbon source and styrene as the sole carbon source, and cultured in a shaking table at a temperature of 32°C and a rotation speed of 130 r / min for 48 hours, in ...
Embodiment 3
[0046] Enrichment culture of specific bacterial flora after screening
[0047] Choose 10 100mL Erlenmeyer flasks and fill them with 50mL LB medium respectively, and fill them with a certain number of polyurethane sponges and Raschig rings as seeds, and then sterilize them. Wait until OD600 in embodiment 2 nm When it is about 0.8, transfer the inorganic salt medium containing only tetrahydrofuran and the inorganic salt medium containing only styrene into the LB medium containing Raschig ring and polyurethane sponge as seeds with an inoculation amount of 5%. , placed in a shaker with a temperature of 37°C and a rotation speed of 180r / min for 18h. During the culture period, measure OD600 every 2 hours nm .
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More