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Extraction process and application of ginseng polypeptide

An extraction process, ginseng technology, applied in the field of extraction process containing ginseng polypeptide, can solve the problems of poor separation effect of ultra-microfiltration membrane, and achieve the effect of improving biological permeability and good bioavailability

Pending Publication Date: 2020-10-02
瑞莱茵(北京)生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on the use of membrane separation technology in the extraction of ginseng polypeptides in the art, the use of ultra-microfiltration membrane separation effect is not good, and the present invention is proposed

Method used

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  • Extraction process and application of ginseng polypeptide
  • Extraction process and application of ginseng polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A process for extracting ginseng polypeptides, comprising the following steps:

[0055] S1 pretreatment: drying the flowers, leaves and roots of ginseng at 10.5°C for 0.5h to obtain raw material A with a moisture content of 7%;

[0056] S2 crushing: crush raw material A to 50 mesh to obtain powder B; drying: dry powder B at 10.5°C for 1 hour to obtain powder C with a moisture content of less than 5%;

[0057] S3 extraction: the powder C is passed through a silica gel column, the extraction temperature is 20°C, the pressure is 5MPa, the extraction time is 1h, the flow rate of ethanol is 500kg / h, and the entrainer ethyl acetate is also added during the extraction. The weight ratio of powder C is 0.3:100; liquid D and residue E are obtained after extraction, residue E is subjected to secondary extraction at a temperature of 20°C and a pressure of 5MPa, the extraction time is 1h, and the flow rate of carbon dioxide is 500kg / h. get the extract;

[0058] S4 Separation: Mix ...

Embodiment 2

[0063] A process for extracting ginseng polypeptides, comprising the following steps:

[0064] S1 pretreatment: drying the flowers, leaves and roots of ginseng at 10.5°C for 0.5h to obtain raw material A with a moisture content of 7%;

[0065] S2 crushing: crush raw material A to 50 mesh to obtain powder B; drying: dry powder B at 10.5°C for 1 hour to obtain powder C with a moisture content of less than 5%;

[0066] S3 extraction: the powder C is passed through a silica gel column, the extraction temperature is 20°C, the pressure is 5MPa, the extraction time is 1h, the flow rate of ethanol is 500kg / h, and the entrainer ethyl acetate is also added during the extraction. The weight ratio of powder C is 0.3:100; liquid D and residue E are obtained after extraction, residue E is subjected to secondary extraction at a temperature of 20°C and a pressure of 5MPa, the extraction time is 1h, and the flow rate of carbon dioxide is 500kg / h. get the extract;

[0067] S4 Separation: Mix ...

Embodiment 3

[0072] A process for extracting ginseng polypeptides, comprising the following steps:

[0073] S1 pretreatment: drying the flowers, leaves and roots of ginseng at 10.5°C for 0.5h to obtain raw material A with a moisture content of 7%;

[0074] S2 crushing: crush raw material A to 50 mesh to obtain powder B; drying: dry powder B at 10.5°C for 1 hour to obtain powder C with a moisture content of less than 5%;

[0075] S3 extraction: the powder C is passed through a silica gel column, the extraction temperature is 20°C, the pressure is 5MPa, the extraction time is 1h, the flow rate of ethanol is 500kg / h, and the entrainer ethyl acetate is also added during the extraction. The weight ratio of powder C is 0.3:100; liquid D and residue E are obtained after extraction, residue E is subjected to secondary extraction at a temperature of 20°C and a pressure of 5MPa, the extraction time is 1h, and the flow rate of carbon dioxide is 500kg / h. get the extract;

[0076] S4 Separation: Mix ...

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Abstract

The invention discloses an extraction process and application of ginseng polypeptide. The extraction process comprises the following steps: S1, pretreatment; S2, crushing; S3, extraction; S4, separation; S5, filtration; and S6, disinfection, wherein a filter membrane used in the step S5 is an alumina carrier prepared by using a specific method and is connected with a covalent organic skeleton filter membrane. Through adjustment of the geometric structure of a covalent organic skeleton and selection of different construction units for adjustment of the pore size and pore shape of the covalent organic skeleton, the filter membrane best suitable for separation of ginseng polypeptide is prepared, and high-efficiency separation and extraction of ginseng polypeptide are realized.

Description

technical field [0001] The invention belongs to the field of ginseng polypeptide preparation, and in particular relates to an extraction process and application of ginseng polypeptide. Background technique [0002] Ginseng has a very special position in the history of Chinese medicine. It is known as the "king of Chinese herbal medicine" and is the representative of Chinese traditional medicine. Modern medical research has proved that ginseng has a good protective effect on the central nervous system, cardiovascular system, respiratory system, blood and hematopoietic system, endocrine system and reproductive system. In addition, studies have proved that ginseng also has significant anti-tumor, anti-radiation, anti-aging and detoxification effects. In the past 30 years, with the development of chemical analysis methods, great progress has been made in the separation, purification and structural identification of ginseng components. It is known that ginseng root contains act...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/14B01D67/00B01D69/10C08G12/08A61K38/01A61K8/64A23L33/18
CPCC07K1/34C07K1/145B01D67/0006B01D69/105C08G12/08A61K38/011A61K8/645A23L33/18A23V2002/00A23V2200/30A23V2250/55
Inventor 方令豪
Owner 瑞莱茵(北京)生物科技有限责任公司
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