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Method for treating microalgae liquid by utilizing membrane bioreactor and algae oil extraction method and device

A membrane bioreactor, microalgae oil technology, which is applied in specific-purpose bioreactors/fermenters, biological material sampling methods, enzyme production/bioreactors, etc., can solve complex processes, low efficiency, and many processes. and other problems, to achieve the effect of high efficiency, low cost and easy automation

Active Publication Date: 2020-10-02
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Therefore, the extraction of algae oil through microalgae cultivation has become one of the current hot spots. However, the currently used algae oil extraction methods or processes generally have the disadvantages of low efficiency, many procedures, complicated processes, and high costs, which need to be improved.

Method used

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  • Method for treating microalgae liquid by utilizing membrane bioreactor and algae oil extraction method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The microalgae fermentation broth of Schizochytrium was crushed. The microalgae fermentation broth was homogeneously crushed under high pressure, the pressure was 40MPa, the number of homogenization was 1 time, the temperature was 40°C, and the pH value was adjusted to 6.0.

[0063] 1) The crushed microalgae fermentation liquid is input into the above-mentioned membrane bioreactor through a feed pump at room temperature for demulsification treatment. The pore size of the PVDF membrane of the hydrophilic material is 0.3 μm, and the ratio of the solidified enzyme to the pool liquid in the membrane bioreactor is 0.7%, the rotation speed of the stirring device is 150rpm, and the aperture of the coarse filter at the overflow port is 500μm.

[0064] 2) After the demulsification, the microalgae liquid flows into the collection tank and is heated, the temperature is controlled at 65°C, the rotation speed is 5rpm, and the material is continuously fed and discharged.

[0065] 3) ...

Embodiment 2

[0068] The microalgae fermentation liquid of Schizochytrium was crushed. The microalgae fermentation liquid was homogeneously crushed under high pressure, the pressure was 60 MPa, the number of homogenization was 2 times, the temperature was 50°C, and the pH value was adjusted to 8.6.

[0069] 1) The crushed microalgae fermentation liquid is input into the above-mentioned membrane bioreactor through a feed pump at room temperature for demulsification treatment. The pore size of the PVDF membrane of the hydrophilic material is 0.2 μm, and the ratio of the solidified enzyme to the pool liquid in the membrane bioreactor is 1%, the rotation speed of the stirring device is 150rpm, and the pore size of the coarse filter at the overflow port is 800μm.

[0070] 2) After the demulsification, the microalgae liquid flows into the collection tank and is heated, the temperature is controlled at 70°C, the rotation speed is 10rpm, and the material is continuously fed and discharged.

[0071]...

Embodiment 3

[0074] The microalgae fermentation liquid of Schizochytrium was crushed. The microalgae fermentation liquid was homogeneously crushed under high pressure, the pressure was 100 MPa, the number of homogenization was 3 times, the temperature was 60°C, and the pH value was adjusted to 10.

[0075] 1) The crushed microalgae fermentation liquid is input into the above-mentioned membrane bioreactor through a feed pump at room temperature for demulsification treatment. The pore size of the PVDF membrane of the hydrophilic material is 0.4 μm, and the ratio of the solidified enzyme to the pool liquid in the membrane bioreactor is 1.5%, the rotation speed of the stirring device is 150rpm, and the aperture of the coarse filter at the overflow port is 700μm.

[0076] 2) After demulsification, the microalgae solution flows into the collection tank and is heated, the temperature is controlled at 75°C, the rotation speed is 20rpm, and the material is continuously fed and discharged.

[0077] ...

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Abstract

The invention discloses a method for treating a microalgae liquid by utilizing a membrane bioreactor. The method comprises the step of performing demulsification treatment on crushed microalgae fermentation liquor by utilizing a membrane bioreactor, wherein a pond liquid in the membrane bioreactor contains immobilized enzyme. According to the scheme, the membrane bioreactor is applied to treatmentof the microalgae fermentation liquor, protein, polysaccharide and other components in the microalgae fermentation liquor are degraded through immobilized enzyme, demulsification is conducted to facilitate separation of microalgae oil, the membrane bioreactor has the advantages of being capable of being continuously used, high in treatment efficiency, good in effect and stable in system, and thealgae oil extraction method and the algae oil extraction device are disclosed.

Description

technical field [0001] The invention relates to the technical field of algae oil extraction, in particular to a method for treating microalgae liquid by using a membrane bioreactor, an algae oil extraction method and a device. Background technique [0002] Microalgae are a class of microorganisms that contain chlorophyll and can perform photosynthesis, and are rich in polyunsaturated fatty acids. Years of research in the medical field have confirmed that polyunsaturated fatty acids (Polyunsaturated fatty acids, PUFAs) can ensure the normal physiological functions of cells, reduce blood cholesterol and triglycerides, improve blood microcirculation, increase brain cell activity, enhance memory and thinking capabilities and many more. Among them, omega-3PUFAs represented by EPA and DHA have become the current research hotspot because of their outstanding effects in promoting brain cell development, stimulating the extension of brain nerve fibers, increasing brain volume, impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/02C11B3/00C12M1/40C12M1/34C12M1/12C12M1/02C12M1/00
CPCC11B1/00C11B1/025C11B3/008C12M21/18C12M23/34C12M23/58C12M27/02C12M27/20C12M29/00C12M29/04C12M33/14C12M41/22C12M41/44C12M47/10
Inventor 张仁辉闫孟可王兆伟
Owner NINGBO FUTIAN BIOTECH
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