Biocontrol agent for controlling pectobacterium atroseptica and preparation method thereof

A bio-control fungicide and a technology for black shank disease are applied in the field of bio-control fungicide for preventing and treating potato black shank disease and the field of preparation thereof, which can solve the problems of less systematic research and the like, and achieve the effect of increasing yield, significant antagonistic effect and potassium-resolving effect. significant effect

Pending Publication Date: 2020-10-13
HUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are relatively few systematic studies on the effects of multi-bacteria compound microecological preparations on plant microecological environment, soil enzyme activity and the relationship between them and disease control.

Method used

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  • Biocontrol agent for controlling pectobacterium atroseptica and preparation method thereof
  • Biocontrol agent for controlling pectobacterium atroseptica and preparation method thereof
  • Biocontrol agent for controlling pectobacterium atroseptica and preparation method thereof

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Embodiment

[0024] A biocontrol agent for preventing and treating potato black shank, the content of live bacillus in each gram of agent is 1×10 8 cfu, the bacillus is a composite bacterium of bacillus pumilus, bacillus megaterium, bacillus amyloliquefaciens, bacillus licheniformis and bacillus coliformis; wherein, the bacillus pumilus, bacillus megaterium, bacillus amyloliquefaciens, lichen The colony-forming effective number (CFU) ratio of Bacillus and Bacillus colloidus was 2.3:2.3:2.0:5.5:5.5.

[0025] A preparation method of a biocontrol fungicide for preventing and treating potato black shank, comprising the following steps:

[0026] a. Filter paper method Apply Bacillus pumilus, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium and Bacillus colioids to the LB solid medium for antagonism test respectively, and set a blank control Group, test result shows that there is no antagonistic effect between each bacillus;

[0027] b. Inoculate the a...

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Abstract

The invention belongs to the technical field of microbial application. According to a biocontrol agent for controlling pectobacterium atroseptica, the content of viable bacilli is larger than or equalto 1 * 108 cfu per gram of bacterial agent, and the bacilli are composite bacilli of bacillus pumilus, bacillus megaterium, bacillus amyloliquefaciens, bacillus licheniformis and bacillus mucilaginosus. The biocontrol agent has good biocontrol and growth promoting effects, and can effectively control pectobacterium atroseptica.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and relates to a biocontrol agent for preventing and treating potato black shank and a preparation method thereof. Background technique [0002] Potato is the fourth largest crop after rice, wheat and corn. China is the largest producer of potatoes. However, bacterial diseases seriously affect potato yield and quality. Among them, potato blackleg is one of the most common bacterial diseases. Potato black shank is a serious disease caused by pectin bacteria, which can occur at the base of potato plant stems and tubers, mainly infecting vascular tissue. From the germination of seed potatoes to the late growth period, the disease can occur, and the seedling stage is the most prosperous. The disease is mainly transmitted through diseased seed potatoes and soil. Its onset is early, its propagation speed is fast, and its control is difficult, which seriously affects the yield and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P21/00C05G3/00C05G3/60C12R1/11C12R1/10C12R1/07
CPCC12N1/20A01N63/22C05G3/00C05G3/60
Inventor 毛露甜林桂格黄子聪陈智飞周星宇陆镇樟刘洁琼杨治
Owner HUIZHOU UNIV
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