Biocontrol agent for controlling pectobacterium atroseptica and preparation method thereof
A bio-control fungicide and a technology for black shank disease are applied in the field of bio-control fungicide for preventing and treating potato black shank disease and the field of preparation thereof, which can solve the problems of less systematic research and the like, and achieve the effect of increasing yield, significant antagonistic effect and potassium-resolving effect. significant effect
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[0024] A biocontrol agent for preventing and treating potato black shank, the content of live bacillus in each gram of agent is 1×10 8 cfu, the bacillus is a composite bacterium of bacillus pumilus, bacillus megaterium, bacillus amyloliquefaciens, bacillus licheniformis and bacillus coliformis; wherein, the bacillus pumilus, bacillus megaterium, bacillus amyloliquefaciens, lichen The colony-forming effective number (CFU) ratio of Bacillus and Bacillus colloidus was 2.3:2.3:2.0:5.5:5.5.
[0025] A preparation method of a biocontrol fungicide for preventing and treating potato black shank, comprising the following steps:
[0026] a. Filter paper method Apply Bacillus pumilus, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium and Bacillus colioids to the LB solid medium for antagonism test respectively, and set a blank control Group, test result shows that there is no antagonistic effect between each bacillus;
[0027] b. Inoculate the a...
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