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Plant extract for inhibiting tyrosinase activity, preparation method and application thereof in cosmetics

A plant extract and tyrosinase technology, which is applied in the field of daily cosmetics, can solve the problems of extracts without tyrosinase activity extracted from Torreya bark, and achieves inhibition of tyrosinase activity, avoidance of damage, excellent Effects of tyrosinase activity

Inactive Publication Date: 2020-10-20
陈阳东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no relevant report on extracting Torreya root bark and obtaining an extract that inhibits tyrosinase activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take dried Torreya root bark and pulverize it into coarse powder, add ethanol aqueous solution with a volume concentration of 80% according to the ratio of solid to liquid of 1kg:8L, and filter at room temperature with 500w ultrasonic extraction for 3 hours. Under the condition of 40°C, the solvent was recovered by drying under reduced pressure to obtain the crude extract of Torreya root bark; (2) Dissolve it in water with 5 times the mass of the crude extract of Torreya root bark, pass it through D101 macroporous resin, and use water and volume fraction respectively 30% ethanol aqueous solution, 70% ethanol aqueous solution, and 95% ethanol aqueous solution are used for gradient elution according to 3 times the column volume, and the eluate of 95% ethanol aqueous solution is collected. Under the conditions of MPa and temperature of 40°C, the solvent was dried under reduced pressure to obtain Torreya root bark extract.

Embodiment 2

[0018] Take the dried torreya root bark and pulverize it into a coarse powder, add an aqueous ethanol solution with a volume concentration of 95% at a material-liquid ratio of 1kg:5L, and filter at room temperature with 500w ultrasonic extraction for 3 hours. The filtrate is vacuumed at 0.02MPa and temperature Dry under reduced pressure at 30°C to recover the solvent, and obtain the crude extract of Torreya root bark; (2) Dissolve it in water with 10 times the mass of the crude extract of Torreya root bark, pass it through D101 macroporous resin, and divide it with water and volume fraction respectively 20% ethanol aqueous solution, 60% ethanol aqueous solution, and 85% ethanol aqueous solution are used for gradient elution according to 2 times the column volume, and the eluate of 85% ethanol aqueous solution is collected, and the vacuum degree is 0.02 Under the conditions of MPa and temperature of 30°C, the solvent was dried under reduced pressure to obtain Torreya root bark e...

Embodiment 3

[0020] Take the dried torreya root bark and pulverize it into a coarse powder, add an aqueous ethanol solution with a volume concentration of 70% according to the ratio of 1kg: 10L of material to liquid, and filter at room temperature with 500w ultrasonic extraction for 3 hours. The filtrate is vacuumed at 0.09MPa and temperature Under the condition of 40°C, the solvent was recovered by drying under reduced pressure to obtain the crude extract of Torreya root bark; (2) Dissolve it in water with 8 times the mass of the crude extract of Torreya root bark, pass it through D101 macroporous resin, and use water and volume fraction respectively 10% ethanol aqueous solution, 45% ethanol aqueous solution, and 80% ethanol aqueous solution are used for gradient elution according to 5 times the column volume, and the 80% ethanol aqueous solution eluate is collected, and the vacuum degree is 0.09 Under the conditions of MPa and temperature of 40°C, the solvent was dried under reduced press...

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Abstract

The invention discloses a plant extract for inhibiting tyrosinase activity, which is prepared by the following steps: extracting torreya grandis root bark at normal temperature by using an ethanol water solution, performing adosprtion by using macroporous resin, performing elution by using ethanol water solutions with different concentrations, collecting 80-95% ethanol water solution eluate, and performing drying. Through normal-temperature extraction and normal-temperature reduced-pressure drying, damage to effective active ingredients in torreya grandis root bark due to too high temperaturein the extraction process is avoided, meanwhile, the concentration of an extraction solvent is screened, macroporous resin and an elution solvent are screened, and the obtained torreya grandis root bark extract has an excellent effect of inhibiting tyrosinase activity; and the half inhibition rate (IC50) reaches 0.63-0.85 mg / ml, and the plant extract can be used as a whitening active ingredient tobe applied to preparation of whitening cosmetics.

Description

technical field [0001] The invention relates to the field of daily cosmetics, in particular to a plant extract for inhibiting tyrosinase activity, a preparation method and its application in cosmetics. Background technique [0002] In recent years, with the improvement of living standards and aesthetic standards, people have begun to pursue higher and higher beauty and skin care, longing for white and clean skin, and whitening cosmetics have become one of the mainstream varieties of skin care cosmetics. Therefore, cosmetics companies and researchers in various countries pay more and more attention to the research and development of whitening cosmetics and skin whitening preparations. [0003] Studies in recent years have proved that hyperpigmentation is caused by ultraviolet rays activating melanin-producing enzymes in melanocytes in the epidermis to produce pigments. In the process of melanin production, tyrosinase converts tyrosine into dopa and dopaquinone, which are pol...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/02
CPCA61K2800/782A61K2800/805A61K2800/82A61Q19/02A61K8/9789
Inventor 陈阳东
Owner 陈阳东
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