Unlock instant, AI-driven research and patent intelligence for your innovation.
Plasma protein marker, detection reagent or reagent tool for diagnosing severe change of new coronal virus pneumonia from mild symptom
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A protein, mild technology
Active Publication Date: 2020-10-20
武汉市金银潭医院
View PDF4 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Currently, there are no biomarkers for the onset, progression and outcome of coronavirusdisease
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0034] Example 1: Mass Spectrometry Analysis of Plasma Samples from Patients with New Coronary Pneumonia
[0035] 1. Experimental materials
[0036] Plasma samples from patients with new coronary pneumonia, from a hospital, including whole blood collected from 10 mild patients (M1-M10, referred to as M) and whole blood collected from 7 severe patients (S1-S7, referred to as S), total 17 whole blood samples. According to the "Diagnosis and Treatment Plan for Pneumonia Infected by Novel Coronavirus (Trial Version 6)" jointly issued by the General Office of the National Health Commission and the Office of the State Administration of Traditional Chinese Medicine, the clinical classification is as follows: mild, mild clinical symptoms, no pneumonia manifestations in imaging ;Severe, meeting any of the following: 1. Shortness of breath, RR≥30 times / min; 2. In a resting state, oxygen saturation ≤93%; 3. Arterial partial pressure of oxygen (Pa02) / inhaled oxygen concentration (Fi02)≤...
Embodiment 2
[0043] Example 2: Machine learning screening for markers from mild disease to severe disease
[0044] 1. Experimental materials
[0045] Mass spectrometry data of 17 plasma samples (see Example 1), Python 3.7 (https: / / www.anaconda.com / ), Scikit learn 0.22.1 (https: / / scikit-learn.org / stable / ). The source code of this experiment is https: / / github.com / Ning-310 / POC-19.
[0046] 2. Experimental process
[0047] (1) To screen for differential proteins, to screen for the absolute value of the fold change (FC) of proteins in the S group and the M group is greater than 0.8, and the two-tailed unpaired Welch T test is less than 0.01 (|log2(FC)|>0.8, unpaired two-sided Welch's t-test, P<0.01).
[0048] (2) Randomly select no more than 5 proteins from the differential proteins to form a potential optimal marker combination (OBC). The initial weight value of each protein is set to 1, and 1000 OBC candidates are set.
[0049] (3) For each candidate OBC, we randomly generate a training d...
Embodiment 3
[0057] Example 3: Validation of OBC Predictor Accuracy
[0058] 1. Experimental materials
[0059] Mass spectrum data of 17 plasma samples (see Example 1), Python 3.7 (https: / / www.anaconda.com / ).
[0060] 2. Experimental process
[0061] (1) Identify the true positive (TruePositive, TP), true negative (True Negative, TN), false positive (False Positive, FP) and false negative (False Negative, FN) ratio for chaoticlogic analysis.
[0062] (2) Draw the chaotic matrix through Scikit learn 0.22.1 software.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to the technical field of biology, in particular to a plasmaprotein marker, a detection reagent or a reagent tool for diagnosing severe change of new coronal virus pneumonia from mild symptoms. The plasmaprotein marker comprises zinc-alpha2-glycoprotein, mucoid 1, mucoid 2 and a complement factor I. Results show that the area (AUC) value under a subject working characteristic curve (ROC) for predicting the severe illness of a patient suffering from mild symptoms through combination of the four proteins is 1, the true positive rate for predicting the severe symptoms of the patient suffering from mild symptoms is 100%, and the false positive rate for predicting the severe symptoms of the patient suffering from mild symptoms is 0. In addition, enzyme-linked immunosorbent assay (ELISA) experiments find that the concentrations of the four proteins have significant differences in blood plasma of patients with mild symptoms and critical patients, so that the accuracy of predicting the severe case of mild symptoms by using the combination is further determined.
Description
technical field [0001] The present invention relates to the field of biotechnology, in particular to plasmaprotein markers, detection reagents or reagent tools for the diagnosis of COVID-19 from mild to severe. Background technique [0002] The novel coronavirusdisease (COVID-19) caused by SARS-CoV-2 infection has a wide range of symptoms, from mild cold and fever, to respiratory syndrome, pneumonia, systemic multiple organ failure and even death; about 10% to 20% Mild patients can develop into severe patients. At present, there are no biomarkers for the occurrence, development and outcome of coronavirusdisease. Contents of the invention [0003] In view of this, the present invention provides plasma protein markers, detection reagents or reagent tools for the diagnosis of COVID-19 from mild to severe. Zinc-alpha-2-glycoprotein, Alpha-1-acid glycoprotein 1, Alpha-1-acid glycoprotein 2, and Complement factor I protein concentrations were significantly different in the ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More