Method for backcross breeding by utilizing plant male sterile material and application

A technology of male sterility and backcross breeding, applied in the field of multi-line variety breeding, can solve the problems of increasing the probability of occurrence of target single plants, heavy breeding workload, and low efficiency

Pending Publication Date: 2020-10-27
江西省超级水稻研究发展中心
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the shortcomings of large workload and low efficiency in the cultivation of existing plant multi-line varieties, and propose a method and application of backcross breeding using plant male sterile materials. A male sterile line, whi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for backcross breeding by utilizing plant male sterile material and application
  • Method for backcross breeding by utilizing plant male sterile material and application
  • Method for backcross breeding by utilizing plant male sterile material and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Using TDR gene loss-of-function recessive male sterile materials for backcross breeding in rice

[0045] Rice is the most important crop in my country, and it is also a typical diploid plant. In the specific embodiments of the present invention, we use rice as the object of implementation to demonstrate the technical solutions of the present invention. Specifically, using the male sterility caused by the TDR gene function loss for backcross breeding, the OsNramp5 mutant gene was introduced into the background parent variety Tainan 11 (a tropical japonica rice variety) to create a low-cadmium Tainan 11 line. Specific steps are as follows:

[0046] 1. Transform the background parent Tainan 11 into a male sterile material

[0047] The TDR gene mutation Tainan 11 male sterile material was created by the CRISPR / Cas9 system-mediated gene knockout technology (for the experimental process and the reproduction of the male sterile material, please refer to the invention pate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

In order to improve the efficiency of backcross work required by cultivation of different strains in cultivation of multi-line varieties, the invention provides a method for backcross breeding by utilizing a plant male sterile material and an application. According to a technical scheme for introducing a nuclear recessive male sterile gene into a background parent of a multi-line variety and assisting backcross breeding, the workload of field artificial hybridization can be greatly reduced, so the scale of backcross groups is easily expanded; the working efficiency of backcross breeding is improved; and the breeding period is shortened. The method provided by the invention is helpful to promote cultivation of the multi-line varieties.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and relates to a method for backcrossing breeding using plant male sterile materials and its application in multi-line variety breeding. Background technique [0002] With the identification and cloning of a large number of favorable plant traits controlling genes (or genetic loci), the breeding application of these genes (or genetic loci) has become the main task of current breeding. Breeding and utilization of known genes (or genetic sites) is mainly carried out through molecular marker-assisted selection. The current mainstream approach is to aggregate a few favorable genes into a certain variety through molecular marker-assisted selection, and with favorable genes With the increase in the number, it becomes very difficult to aggregate them into one species. It is even more difficult to aggregate different genes located on the same chromosome into one species. But cultivating multi-line var...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 龙起樟黄永兰万建林王会民芦明唐秀英朱雪晶
Owner 江西省超级水稻研究发展中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap