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High-protein probiotic feed prepared from soy sauce residues by using solid-state fermentation method and preparation method of high-protein probiotic feed

A technology of solid-state fermentation and probiotics, applied in animal feed, animal feed, application, etc., can solve the problems of high water content, easy to spoil, low degree of reuse, etc., achieve rich nutrition, improve palatability and digestibility , the effect of extending the shelf life

Pending Publication Date: 2020-10-30
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sauce residue is rich in nutrients, and sauce residue (dry basis) is rich in protein (25~30%), crude fiber (13~25%), crude fat (7~10%), minerals (8~16%) %) and other nutrients, but its water content is high, it is easy to spoil and deteriorate, and the degree of reuse is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A high-protein probiotic feed prepared by solid-state fermentation using sauce residue as raw material and a preparation method thereof, comprising the following steps:

[0028] 1) Prepare sauce residue fermentation medium, mix 47 parts of sauce residue, fermentable sugar glucose and lactose according to the ratio of 1:1, add 4 parts, add water according to the ratio of 1:0.8, natural pH, high pressure steam After sterilization, the initial sauce medium was obtained.

[0029] 2) Prepare probiotic seed medium, MRS liquid medium and YPD liquid medium, inoculate the activated probiotic strains into the seed medium according to 1% inoculum amount, cultivate lactic acid bacteria at 37°C for 12~20h, yeast Cultivate at 28°C for 24-36 hours, and shake once every 4 hours during the cultivation process to obtain the probiotic bacteria liquid.

[0030] 3) Inoculate 15% of the inoculation amount of probiotic bacteria liquid into the sauce medium in step 1) with yeast and lactic ac...

Embodiment 2

[0033] A high-protein probiotic feed prepared by solid-state fermentation using sauce residue as raw material and a preparation method thereof, comprising the following steps:

[0034] 1) Prepare sauce residue fermentation medium, mix 39 parts of sauce residue, 4 parts of fermentable sugar glucose and lactose in a ratio of 1:1, add 47 parts of water according to the ratio of material to water ratio of 1:1.2, natural pH, high-pressure steam After sterilization, the initial sauce medium was obtained.

[0035] 2) Prepare probiotic seed medium, MRS liquid medium and YPD liquid medium, inoculate the activated probiotic strains into the seed medium according to 1% inoculum amount, cultivate lactic acid bacteria at 37°C for 12~20h, yeast Cultivate at 28°C for 24-36 hours, and shake once every 4 hours during the cultivation process to obtain the probiotic bacteria liquid.

[0036] 3) Inoculate 10% of the inoculum amount of probiotic bacteria liquid into the sauce medium in step 1) at...

Embodiment 3

[0039] A high-protein probiotic feed prepared by solid-state fermentation using sauce residue as raw material and a preparation method thereof, comprising the following steps:

[0040] 1) Prepare sauce residue fermentation medium, mix 38 parts of sauce residue, 4 parts of fermentable sugar glucose and lactose in a ratio of 1:1, add 38 parts of water in a ratio of 1:1, natural pH, high-pressure steam After sterilization, the initial sauce medium was obtained.

[0041] 2) Prepare probiotic seed medium, MRS liquid medium and YPD liquid medium, inoculate the activated probiotic strains into the seed medium according to 1% inoculum amount, cultivate lactic acid bacteria at 37°C for 12~20h, yeast Cultivate at 28°C for 24-36 hours, and shake once every 4 hours during the cultivation process to obtain the probiotic bacteria liquid.

[0042] 3) Inoculate 20% of the inoculum amount of probiotic bacteria liquid into the sauce medium in step 1) at a ratio of 1:1 between yeast and lactic ...

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Abstract

The invention provides a high-protein probiotic feed prepared from soy sauce residues through a solid-state fermentation method and a preparation method of the high-protein probiotic feed, and belongsto the technical field of fermented feeds. The preparation method of the high-protein probiotic feed comprises the following steps: mixing 30-50 parts of sauce residues and 2-5 parts of a mixture ofglucose / lactose (1: 1) with 25-40 parts of water, uniformly mixing the raw materials in parts by weight, inoculating 5-15 parts of probiotics, inoculating saccharomycetes, inoculating lactic acid bacteria in a ratio of 1: 1 after 24 hours, regulating and controlling the temperature in multiple steps to be within the range of 30-37 DEG C, increasing the temperature by 2 DEG C every 12 hours, and fermenting for 24-60 hours to obtain the high-protein probiotic feed. According to the method, the solid-state fermentation sauce residues are regulated and controlled through a delayed inoculation technology and multi-step temperature; the problem of waste of the sauce residues is effectively solved, meanwhile, the high-protein probiotic feed with the dry basis crude protein content as high as 38.86% and the probiotic viable count as high as 7.2*10 <8> CFU*mL <-1> is prepared, the digestibility and palatability of the sauce residues are improved, the feed cost can also be reduced, and the immunocompetence of fed animals is improved.

Description

technical field [0001] The invention relates to the technical field of fermented feed and the technical field of solid waste recycling, in particular to a high-protein probiotic feed, and also relates to a method for preparing high-protein probiotic feed by using waste sauce residue. Background technique [0002] Soy sauce residue, usually also called soy sauce residue, refers to the final solid by-product produced after a series of production processes in the process of brewing soy sauce. In 2014 alone, China produced nearly 8 million tons of soy sauce, and about 5.5 million tons of sauce residue. And the annual growth rate of soy sauce market is more than 10%, so the output of soy sauce residue in my country is very huge. [0003] Sauce residue is rich in nutrients, and sauce residue (dry basis) is rich in protein (25~30%), crude fiber (13~25%), crude fat (7~10%), minerals (8~16%) %) and other nutrients, but its water content is high, it is easy to spoil and deteriorate,...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/38A23K20/163A23L3/3571
CPCA23K10/12A23K10/38A23K20/163A23L3/3571Y02P60/87
Inventor 赵新河贺壮壮任晓洁陆文伟马成业
Owner SHANDONG UNIV OF TECH
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