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Preparation method of gold-silver core-shell nanoparticle electrochemical biosensor synthesized by adopting pressure change type micro-fluidic chip

A technology of biosensors and microfluidic chips, applied in chemical instruments and methods, laboratory containers, electrochemical variables of materials, etc., can solve the problems of high experimental cost, long detection time, and low sensitivity

Inactive Publication Date: 2020-10-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for detecting procalcitonin mainly include radioimmunoassay, colloidal gold colorimetric method, etc., but these detection methods have disadvantages such as complicated operation, long detection time, low sensitivity, and high experimental cost.

Method used

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  • Preparation method of gold-silver core-shell nanoparticle electrochemical biosensor synthesized by adopting pressure change type micro-fluidic chip
  • Preparation method of gold-silver core-shell nanoparticle electrochemical biosensor synthesized by adopting pressure change type micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of gold-silver core-shell nanoparticles by pressure-variable microfluidic chip

[0030] Configure the required gold nano-seed solution and the growth solution of the gold nano-seed, take a certain volume of silver nitrate solution; the gold nano-seed solution and the growth solution of the gold nano-seed are respectively passed into the secondary inlet (2), (3), the silver nitrate solution is passed into the primary inlet (1); the two solutions at the secondary inlet react first in the continuous phase channel (4) before the "cross" structure to form triangular gold nanoseeds, and in the channel (5 ) in silver nitrate reacts on the generated gold nano-seeds; the product is collected at the product outlet (6) and placed in a water bath at 60°C for 5 hours;

[0031] After the fluid is passed into the microfluidic chip, the air channel is regulated by the air channel adjustment device: the air channel (7), (9), (11) is closed at the same time, and the...

Embodiment 2

[0034] Example 2 Preparation of gold-silver core-shell nanoparticles by pressure-variable microfluidic chip

[0035]Configure the required gold nano-seed solution and the growth solution of the gold nano-seed, take a certain volume of silver nitrate solution; the gold nano-seed solution and the growth solution of the gold nano-seed are respectively passed into the secondary inlet (2), (3), the silver nitrate solution is passed into the primary inlet (1); the two solutions at the secondary inlet react first in the continuous phase channel (4) before the "cross" structure to form triangular gold nanoseeds, and in the channel (5 ) in silver nitrate reacts on the generated gold nano-seeds; the product is collected at the product outlet (6) and placed in a 70°C water bath for 4 hours;

[0036] After the fluid is passed into the microfluidic chip, the air channel is regulated by the air channel adjustment device: the air channel (7), (9), (11) is closed at the same time, and the air...

Embodiment 3

[0039] Example 3 Preparation of gold-silver core-shell nanoparticles by pressure-variable microfluidic chip

[0040] Configure the required gold nano-seed solution and the growth solution of the gold nano-seed, take a certain volume of silver nitrate solution; the gold nano-seed solution and the growth solution of the gold nano-seed are respectively passed into the secondary inlet (2), (3), the silver nitrate solution is passed into the primary inlet (1); the two solutions at the secondary inlet react first in the continuous phase channel (4) before the "cross" structure to form triangular gold nanoseeds, and in the channel (5 ) in silver nitrate reacts on the generated gold nano-seeds; the product is collected at the product outlet (6) and placed in a water bath at 80°C for 5 hours;

[0041] After the fluid is passed into the microfluidic chip, the air channel is regulated by the air channel adjustment device: the air channel (7), (9), (11) is closed at the same time, and the...

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Abstract

The invention relates to a preparation method of a gold-silver core-shell nanoparticle electrochemical biosensor synthesized by adopting a pressure change type micro-fluidic chip, and belongs to the field of micro-fluidic synthesis and electrochemical sensors. The gold and silver core-shell nanoparticles synthesized by adopting the pressure change type micro-fluidic chip have the characteristics of controllable morphology, adjustable core-shell thickness and large specific surface area. The gold-silver core-shell nanoparticles are used as the substrate of the electrochemical biosensor, are used for detecting procalcitonin, and have the advantages of high sensitivity and favorable specificity.

Description

technical field [0001] The invention relates to a method for preparing an electrochemical biosensor of gold and silver core-shell nanoparticles synthesized by a pressure-variable microfluidic chip, and belongs to the field of microfluidic synthetic nanomaterials and electrochemical sensors. Background technique [0002] Procalcitonin (PCT) is an endogenous non-steroidal anti-inflammatory substance that is produced when infected by bacteria, among which bacterial endotoxin is the most important stimulating factor to induce PCT production. In general, the concentration of PCT in the blood of healthy people is less than 0.2 μg / L, and it is usually not detected. When bacterial infection occurs, in addition to the thyroid gland, macrophages and monocytes in the liver, lymphocytes and endocrine cells in the lung and intestinal tissue can synthesize and secrete PCT, and the level of PCT in the blood will increase significantly. PCT is an innovative diagnostic index for early diagn...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327B01L3/00
CPCG01N27/308G01N27/3278B01L3/5027
Inventor 魏琴郭玉健任祥代笠许坤马洪敏吴丹孙旭
Owner UNIV OF JINAN
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