Characteristic chromatogram establishing method of traditional Chinese medicine or traditional Chinese medicine composition preparation, and application

A characteristic map and method establishment technology, which is applied in the field of establishing characteristic maps of traditional Chinese medicine or traditional Chinese medicine composition preparations by high performance liquid chromatography, can solve problems such as the difficulty of controlling the quality of Chinese medicine and the difficulty of increasing the difficulty of complex analysis of Chinese medicine components

Active Publication Date: 2020-10-30
BEIJING TONGRENTANG CO LTD
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Problems solved by technology

[0002] Traditional Chinese medicine has the characteristics of multiple chemical components and multiple sources of medicine at the same time, which leads to the complexity of the quality control of traditional Chinese medicines; Chinese medicine compound preparations are composed of multi-flavored Chinese medicines, which not only increases the difficulty of complex analysis of Chinese medicine components, but also makes it difficult to It is more difficult to control the quality of each raw material Chinese medicine in the finished Chinese medicine compound preparation

Method used

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  • Characteristic chromatogram establishing method of traditional Chinese medicine or traditional Chinese medicine composition preparation, and application
  • Characteristic chromatogram establishing method of traditional Chinese medicine or traditional Chinese medicine composition preparation, and application
  • Characteristic chromatogram establishing method of traditional Chinese medicine or traditional Chinese medicine composition preparation, and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] 1 Instruments and reagents

[0115] 1.1 Instrument

[0116]Waters Acquity HClass ultra-high performance liquid chromatography (including quaternary solvent manager, PAD detector, online temperature controller, sample manager, Empower workstation); ultrasonic cleaner (KQ-250DE CNC ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.); Mettler ML 204 electronic analytical balance (Swiss Mettler-Tolly Group); Mettler XP 205 electronic analytical balance (Swiss Mettler-Tolly Group), 0.22μm microporous membrane (Tianjin Jinteng Experimental Equipment Co., Ltd.).

[0117] 1.2 Reagent

[0118] Acetonitrile (Merck, chromatographically pure), anhydrous formic acid (analytical pure, Tianjin Guangfu Institute of Fine Chemicals), ultrapure water (milli-Q ultrapure water instrument). Reference substances: berberine hydrochloride (content 86.8%, batch number 110713-201613;), palmatine hydrochloride (content 86.8%, batch number 110732-201611), berberine hydrochloride (conten...

Embodiment 2

[0151] Embodiment 2, different sample detection

[0152] Adopt established analytical method, analyze and measure five batches of (natural) ANW simultaneously, measurement result is shown in Table 10, and characteristic collection is with berberine hydrochloride chromatographic peak area as reference, calculates the relative peak area of ​​35 characteristic chromatographic peaks, Arrange the analysis according to the source information of the characteristic peaks.

[0153] Table 10 Five batches of (natural) ANW characteristic map measurement results

[0154]

[0155]

[0156]The method for establishing characteristic maps of traditional Chinese medicine or Chinese medicine composition preparations of the present invention can be effectively used for the characteristics of Chinese medicine or Chinese medicine composition preparations containing natural bezoar, bezoar, natural musk, musk, scutellaria baicalensis, berberine, gardenia and / or turmeric The map is created.

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Abstract

The invention provides a characteristic chromatogram establishing method of a traditional Chinese medicine or a traditional Chinese medicine composition preparation, and application. The characteristic chromatogram establishing method comprises the following steps of: extracting the traditional Chinese medicine or the traditional Chinese medicine composition preparation by using a solvent to prepare a test solution; and carrying out high performance liquid chromatography determination on the test solution, and establishing a characteristic chromatogram, wherein acetonitrile(A)-acid aqueous solution(B) is used as a mobile phase, gradient elution is adopted, and the detection wavelength is 190-500 nm. The characteristic chromatogram establishing method of the traditional Chinese medicine ortraditional Chinese medicine composition preparation is clear in characteristic peak attribution and free of negative interference, and can be used for establishing the characteristic chromatogram ofthe traditional Chinese medicine or traditional Chinese medicine composition preparation containing natural calculus bovis, artificial calculus bovis, natural musk, artificial musk, scutellaria baicalensis, coptis chinensis, cape jasmine fruits and radix curcumae.

Description

technical field [0001] The present invention relates to a method for establishing a characteristic map of a traditional Chinese medicine or a Chinese medicine composition preparation and its application, in particular to a method for establishing a characteristic map of a Chinese medicine or a Chinese medicine composition preparation by high performance liquid chromatography, and using said The application of the characteristic map analysis of traditional Chinese medicine components belongs to the technical field of traditional Chinese medicine. Background technique [0002] Traditional Chinese medicine has the characteristics of multiple chemical components and multiple sources of medicine at the same time, which leads to the complexity of quality control of traditional Chinese medicines; Chinese medicine compound preparations are composed of multi-flavored Chinese medicines, which not only increases the difficulty of complex analysis of Chinese medicine components, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74Y02A50/30
Inventor 张荣寰彭平杜菁刘博譞常宇谷彩云
Owner BEIJING TONGRENTANG CO LTD
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