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Quality control method for non-volatile components of traditional Chinese medicine sachet, quality control spectrogram and construction method

A quality control method and non-volatile technology, which can be used in measurement devices, material separation, and analysis of materials, etc., can solve the problems of reducing detection efficiency, poor stability and repeatability of test results, and increasing the cost of instrument use, achieving improved Detection efficiency, improve instrument utilization efficiency, and avoid the effect of continuous use of the instrument

Pending Publication Date: 2020-11-24
湖南旻一科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This mode requires repeated detection of samples according to thin-layer chromatography. The operation is complicated, the workload is heavy, and it is easily affected by human beings. The stability and repeatability of the detection results are not good.
At the same time, due to the complexity of the ingredients contained in Chinese herbal medicines, there may be a variety of medicinal materials containing the same non-volatile ingredients. Therefore, only using a single ingredient as a standard cannot effectively achieve the quality control of traditional Chinese medicine sachets.
[0003] Moreover, the properties, polarity, and acidity and alkalinity of different medicinal materials are quite different, and it is difficult to reflect them simultaneously in the chromatogram of the same condition. However, the separate detection of multiple components will undoubtedly increase the complexity of quality control and detection. cost
Moreover, in the same compound composition, the content of markers of different Chinese herbal medicines varies greatly, and cannot be unified in the same detection range, but changing the setting of different detection conditions for different content ranges increases the use cost of the instrument and reduces the detection rate. efficiency

Method used

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  • Quality control method for non-volatile components of traditional Chinese medicine sachet, quality control spectrogram and construction method

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Embodiment 1

[0056] A quality control method for non-volatile components of traditional Chinese medicine sachets, quality control spectrum and construction method: Chromatographic conditions: using octadecylsilane bonded silica gel as a filler (column length is 250mm, inner diameter is 4.6mm, particle diameter 5 μm); with acetonitrile as mobile phase A, with 0.1% phosphoric acid solution as mobile phase B, carry out gradient elution as specified in the table below; carry out gradient elution as specified in table 1; flow rate is 1.0mL / min; The column temperature is 30°C; the detection wavelength is 203nm; the number of theoretical plates should not be less than 2000 based on the peak of dihydromyricetin.

[0057] Table 1

[0058]

[0059] Preparation of the reference substance solution: Take an appropriate amount of reference substances of dihydromyricetin, cinnamon aldehyde, and emodin, weigh them accurately, add methanol to make a 50 μg / mL solution, shake well, and use it as the refer...

Embodiment 2

[0064] A quality control method, quality control spectrum and construction method of non-volatile components in traditional Chinese medicine sachets:

[0065] Chromatographic conditions: use octadecylsilane bonded silica gel as filler (column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); acetonitrile is used as mobile phase A, 0.1% phosphoric acid solution is used as mobile phase B, press Carry out gradient elution according to the provisions in the table below; carry out gradient elution according to the provisions in Table 1; flow rate is 1.0mL / min; column temperature is 30°C; detection wavelength is 203nm; theoretical plate number is calculated according to the peak of dihydromyricetin Should not be less than 2000.

[0066] Table 1

[0067]

[0068] Preparation of reference substance solution: Take an appropriate amount of ginsenoside Rg1 reference substance, weigh it accurately, add methanol to make a 50 μg / mL solution, shake well, and use it as the ...

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Abstract

The invention discloses a quality control method for non-volatile components of a traditional Chinese medicine sachet, a quality control spectrogram and a construction method, and belongs to the fieldof medicine quality control. The quality control method comprises the step of simultaneously detecting the components of rheum officinale, angelica sinensis, ampelopsis grossedentata, immature bitterorange, rosemary, cinnamon and atractylodes lancea in the traditional Chinese medicine sachet by adopting an LC method, so that synchronous detection and identification of various non-volatile components in the traditional Chinese medicine sachet under single measurement are realized, and the detection efficiency is improved. The ginseng component in the traditional Chinese medicine sachet is independently detected by adopting the same LC method, so that the instrument utilization efficiency of daily detection is improved. Furthermore, the characteristic peaks in the quality control spectrogram of the traditional Chinese medicine sachet are attributed and analyzed, the attribution of decoction pieces with the peaks of 1-12 is defined, and a foundation is laid for constructing the spectraleffect relationship of the traditional Chinese medicine sachet containing the components of rheum officinale, angelica sinensis, ampelopsis grossedentata, fructus aurantii immaturus, rosemary, cinnamon and atractylodes lancea.

Description

technical field [0001] The invention belongs to the field of medicine quality control, and in particular relates to a quality control method of non-volatile components in traditional Chinese medicine sachets, a quality control spectrum and a construction method. Background technique [0002] The traditional Chinese medicine sachet involved in the invention is mainly made of 13 medicinal materials such as ginseng, rhubarb, angelica, vine tea, citrus aurantium, rosemary, cinnamon, and atractylodes. The current drug standards mainly use thin-layer chromatography to realize the detection and identification of multi-flavor medicinal materials in sachets with the identification mode of single component or main spot. This mode requires repeated detection of samples according to thin-layer chromatography. The operation is complex, the workload is heavy, and it is easily affected by human beings. The stability and repeatability of the detection results are not good. At the same time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/88G01N30/8679G01N2030/8809G01N2030/027
Inventor 李博之周芬
Owner 湖南旻一科技有限公司
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