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Method for detecting various mycotoxins based on Raman spectrum of gold nanoparticles

A mycotoxin and surface-enhanced Raman technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of ultra-sensitive Raman spectrum detection, such as complex detection, time-consuming and labor-intensive, application limitations, etc., to achieve low cost, improve application characteristics, The effect of improving specificity and precision

Pending Publication Date: 2022-08-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the preparation process is not only time-consuming and labor-intensive, but also costly, so that AFB 1 The disadvantages of ultrasensitive Raman spectroscopy detection are complex and expensive, which greatly restricts its application in real-time detection of food
At the same time, most of the SERS substrates for these mycotoxins are prepared by combining specific antibodies or aptamers with noble metal colloids. Different types of antibodies and DNA are required for different substances, and metal colloids without any modification can easily and quickly recognize a variety of mycotoxins. Research on mycotoxins is rarely reported

Method used

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  • Method for detecting various mycotoxins based on Raman spectrum of gold nanoparticles
  • Method for detecting various mycotoxins based on Raman spectrum of gold nanoparticles
  • Method for detecting various mycotoxins based on Raman spectrum of gold nanoparticles

Examples

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Embodiment 1

[0048] A preparation method of a surface-enhanced Raman detection substrate, comprising the following steps:

[0049] (1) 1 mL of trisodium citrate (20 mg / mL) was added to 98.9 mL of distilled water, heated to boiling in boiling water, and 100 μL of 250 mM tetrachloroauric acid solution was rapidly added under vigorous stirring and kept boiling for 3 min to obtain particle size Smaller gold nanoparticle solutions;

[0050] (2) placing the gold nanoparticles obtained in step (1) in a refrigerator at 4° C. for 24 hours to obtain a gold seed solution;

[0051] (3) at room temperature, take 1 mL of the gold seed solution obtained in step (2) and add it to 40 mL of distilled water, add 400 μL of 1w / w% trisodium citrate solution and 400 μL of 1w / w% hydroxylamine hydrochloride solution in turn, and react with rapid stirring at constant temperature for 6 min; 80 μL of tetrachloroauric acid solution (240.44 mM) was quickly added thereto to obtain a surface-enhanced Raman detection sub...

Embodiment 2

[0054] A preparation method of a surface-enhanced Raman detection substrate, comprising the following steps:

[0055] (1) 1 mL of trisodium citrate (25 mg / mL) was added to 98.9 mL of distilled water, heated to boiling in boiling water, rapidly added 100 μL of 250 mM tetrachloroauric acid solution under vigorous stirring and kept boiling for 4 min to obtain particle size Smaller gold nanoparticle solutions;

[0056] (2) placing the gold nanoparticles obtained in step (1) in a refrigerator at 4° C. for 24 hours to obtain a gold seed solution;

[0057] (3) At room temperature, 1 mL of the gold seed solution obtained in step (2) was added to 37.4 mL of distilled water, followed by adding 400 μL 1w / w% trisodium citrate solution and 400 μL 1w / w% hydroxylamine hydrochloride solution, and the reaction was stirred rapidly at a constant temperature 5 min; 80 μL of tetrachloroauric acid solution (240.44 mM) was quickly added thereto to obtain a surface-enhanced Raman detection substrate...

Embodiment 3

[0059] A preparation method of a surface-enhanced Raman detection substrate, comprising the following steps:

[0060] (1) 1 mL of trisodium citrate (30 mg / mL) was added to 98.9 mL of distilled water, heated to boiling in boiling water, and 100 μL of 250 mM tetrachloroauric acid solution was rapidly added under vigorous stirring and kept boiling for 17 min to obtain a particle size Smaller gold nanoparticle solutions;

[0061] (2) placing the gold nanoparticles obtained in step (1) in a refrigerator at 4° C. for 24 hours to obtain a gold seed solution;

[0062] (3) At room temperature, take 1 mL of the gold seed solution obtained in step (2) and add it to 38 mL of distilled water, add 400 μL of 1.5w / w% trisodium citrate solution and 400 μL of 1.5w / w% hydroxylamine hydrochloride solution in turn, and stir rapidly at a constant temperature The reaction was carried out for 5 min; 80 μL of tetrachloroauric acid solution (240.44 mM) was quickly added thereto to obtain a surface-enh...

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Abstract

The invention belongs to the technical field of biological detection, discloses a method for detecting various mycotoxins based on Raman spectrum of gold nanoparticles, and particularly discloses a preparation method of a surface-enhanced Raman detection substrate, which comprises the following steps: mixing a gold seed solution with a reducing agent and a gold salt solution to obtain the surface-enhanced Raman detection substrate. According to the method, a gold salt solution is reduced through chemical reaction, the particle size and roughness of gold seeds are enlarged through a seed crystal growth technology, the surface-enhanced Raman detection substrate with a good mycotoxin recognition specific functional group is obtained, the specificity and accuracy of mycotoxin detection are further improved, and the application prospect is wide. Therefore, the application characteristic of SERS detection of mycotoxin is improved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting various mycotoxins by Raman spectroscopy based on gold nanoparticles. Background technique [0002] Mycotoxins are secondary metabolites naturally produced by fungi of the genera Aspergillus, Penicillium, Fusarium, and Alternaria. The warm and humid environment favors the growth and reproduction of these fungi, resulting in mycotoxins contamination and serious economic losses. Many crops can be infected by these fungi, including grains, oilseeds, fruits, and nuts, so mycotoxins can be found in varying concentrations in agricultural products and their derivatives. Therefore, sensitive and rapid detection of mycotoxins is very important. To date, various analytical techniques have been used for aflatoxin B 1 ((Aflatoxins B1, AFB 1 ), Deoxynivalenol (DON) detection, such as high performance liquid chromatography (High Performance L...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 尹寿伟王书馨席永康魏涛杨晓泉
Owner SOUTH CHINA UNIV OF TECH
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