Plant print identification method for effective gold plating of scanning electron microscope
An electron microscope and identification method technology, applied in the field of becoming clear, can solve the problems of inability to obtain evidence by sub-microscopic photography, limit the process of plant pattern identification and solving cases, and cannot perform film processing, etc., so as to increase the accuracy of plant pattern identification and improve Identify the effect of the effect
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Embodiment 1
[0035] see figure 1 Scanning electron microscope examination material (ruler = 200 μm) of tiny plant fragments on the clothes of a corpse of a certain case, the structure is unclear after ion sputtering gold plating;
[0036] 1) Put 2 pieces of plant debris on the clothes of the corpse, soak them in clean water for 2-4 hours respectively;
[0037] 2) Take out the plant debris, put it in FAA fixative solution, and fix it for 48 hours;
[0038] 3) Dehydrate the plants in step 2), namely: use 65%, 765%, 85%, 95% ethanol water with different concentrations, and each gradient interval is 2 hours;
[0039] 4) Put the plant in step 3) into 100% dehydration for 1 hour, then take it out and put it into acetone to continue dehydration for 30 minutes;
[0040] 5) Apply co 2 Critical point drying method, the plants in step 4) are subjected to critical point drying;
[0041] 6) Using a double-sided blade, for the plants in step 5), cut the double-sided blade at the 90 °C angle perpendi...
Embodiment 2
[0048] Figure 4 For the tiny fragments of plants adhering to the coat of a corpse in a certain case, the scanning electron microscope experiment (ruler = 100 μm), after the gold coating, the internal structure could not be clearly seen;
[0049] Utilize a kind of scanning electron microscope plant pattern identification method provided by the invention, comprising the following steps:
[0050] 1) 2 pieces of plant debris adhered to the coat of the corpse were soaked in clean water for 2-4 hours respectively;
[0051] 2) Take out the plant debris, put it in FAA fixative solution, and fix it for 48 hours;
[0052] 3) Dehydrate the plants in step 2), namely: use 65%, 765%, 85%, 95%, ethanol water of different concentrations, and each gradient interval is 2 hours;
[0053] 4) Put the plant in step 3) into 100% dehydration for 1 hour, then take it out and put it into acetone to continue dehydration for 30 minutes;
[0054] 5) Apply co 2 Critical point drying method, the plants...
Embodiment 3
[0061] see Figure 7 Scanning electron microscopy of tiny plant fragments on the clothes of a corpse (ruler = 120 μm), the structure is unclear after ion sputtering gold plating;
[0062] A scanning electron microscope plant pattern identification method provided by the invention comprises the following steps:
[0063] 1) Put 2 pieces of plant debris on the clothes of the corpse, soak them in clean water for 2-4 hours respectively;
[0064] 2) Take out the plant debris, put it in FAA fixative solution, and fix it for 48 hours;
[0065] 3) Dehydrate the plants in step 2), namely: use 65%, 765%, 85%, 95%, ethanol water of different concentrations, and each gradient interval is 2 hours;
[0066] 4) Put the plant in step 3) into 100% dehydration for 1 hour, then take it out and put it into acetone to continue dehydration for 30 minutes;
[0067] 5) Apply co 2 Critical point drying method, the plants in step 4) are subjected to critical point drying;
[0068] 6) Using a double...
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