Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of protocatechuic acid in antagonizing feed fumonisins

A technology of fumonisin and protocatechuic acid, which can be used in medical preparations containing active ingredients, climate change adaptation, food ingredients containing natural extracts, etc., can solve the problem of affecting the titer of vaccine antibodies and reducing vaccine-specific antibody responses and other problems, to improve the survival rate and production performance, reduce the morbidity and death rate, and inhibit the effects of livestock and poultry diseases.

Active Publication Date: 2022-05-10
CHINA AGRI UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taranu et al found that FB1 will reduce the vaccine-specific antibody response during vaccination, affecting the antibody titer of the vaccine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of protocatechuic acid in antagonizing feed fumonisins
  • Application of protocatechuic acid in antagonizing feed fumonisins
  • Application of protocatechuic acid in antagonizing feed fumonisins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Application of protocatechuic acid in antagonizing fumonisins

[0028] 1 test material

[0029] 1.1 Test reagents

[0030] Fumonisin FB1 standard (>98%) and FB1 monoclonal antibody (potency 1:2 10 , OD450=1), purchased from Shandong Lvdu Biotechnology Co., Ltd.

[0031] Protocatechuic acid (HPLC>98%), purchased from TCIA Chemical Industry Development Co., Ltd.

[0032] Fumonisin FB1 detection kit was purchased from Beijing Qinbang Biotechnology Co., Ltd.

[0033] Water-soluble silymarin, containing 95% silybin, was purchased from Panjin Tianyuan Pharmaceutical Co., Ltd.

[0034] 2 methods

[0035] 2.1 In vitro antagonism scheme of fumonisins

[0036] 2.1.1 Detoxification test of protocatechuic acid on fumonisin FB1 under different temperature and time conditions

[0037] Add 100μL 0.1M protocatechuic acid solution to 1mL 1ppm fumonisin FB1 solution and mix to make the FB1 concentration 0.91ppm), and act at 22~25℃, 25~28℃ and 28~32℃ for 0.5h, 1h and 2h r...

Embodiment 2

[0102] Example 2 Protocatechuic acid antagonizes the application of fumonisins in feed

[0103] Purpose of the experiment: To establish the chicken embryo FB1 toxin model, determine the LD50 of fumonisin FB1 and observe the target organs; further compare the detoxification effects of protocatechuic acid, fumonisin FB1 monoclonal antibody, and silymarin on FB1 based on the FB1 chicken embryo model . The experiment used chicken embryo mortality, lung and gastrointestinal pathological changes and toxin residues as indicators to evaluate the detoxification effect of protocatechuic acid.

[0104] 1. Reagents and Materials

[0105] 1.1 Reagents

[0106] Fumonisin B1 standard (>98%) and FB1 monoclonal antibody (potency 1:2 10 , OD450=1), purchased from Shandong Lvdu Biotechnology Co., Ltd.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
melting pointaaaaaaaaaa
Login to View More

Abstract

The present invention provides a new application of protocatechuic acid (3,4 dihydroxybenzoic acid) in antagonizing fumonisins. Protocatechuic acid is used as an antagonist of fumonisin B1, which can resist fumonisin, the main mycotoxin in feed. The activity of toxin B1 can effectively inhibit the diseases of livestock and poultry caused by fumonisin B1, thereby greatly reducing the incidence and mortality of livestock and poultry, and effectively improving the survival rate and production performance of livestock and poultry.

Description

technical field [0001] The invention relates to a new application of protocatechuic acid, in particular to the application of protocatechuic acid in antagonizing fumonisins in feed. Background technique [0002] Protocatechuic acid (PCA), chemical name 3,4-dihydroxybenzoic acid, structure as shown in formula I, CAS registration number is 99-50-3, molecular formula is (HO)2C6H3COOH, molecular weight: 154.12. [0003] [0004] The compound is a white to brown crystalline powder that changes color in air. Density 1.54g / cm3. The melting point is about 200°C (decomposition). Soluble in 50 parts of water; Soluble in ethanol, ether. [0005] Protocatechuic acid exists in the leaves of red plums, fern and holly plants. Protocatechuic acid has a variety of biological activities against different molecular targets, and has antibacterial, antioxidant, anti-inflammatory, anti-hyperglycemic and neuroprotective effects. In addition, protocatechuic acid has a potential chemoprotect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/192A23L5/20
CPCA23L5/27A23V2002/00A23V2250/21Y02A40/70
Inventor 何诚何润春张宇航王艺晖
Owner CHINA AGRI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com